Guinea pig Anti-TRPV1 (VR1) Antibody | Alomone Labs ACC-030-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-TRPV1 (VR1) Antibody

catalog :

ACC-030-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Zhang W, Cao H, Li Y, Fu X, Zhang Y. Peripheral ablation of type III adenylyl cyclase induces hyperalgesia and eliminates KOR-mediated analgesia in mice. JCI Insight. 2022;7: pubmed publisher
Liu B, Cao Q, Zhao X, Liu H, Zhang Y. Inhibition of TRPV1 by SHP-1 in nociceptive primary sensory neurons is critical in PD-L1 analgesia. JCI Insight. 2020;5: pubmed publisher
Taylor T, Konda P, John S, Bulmer D, Hockley J, Smith E. Galanin suppresses visceral afferent responses to noxious mechanical and inflammatory stimuli. Physiol Rep. 2020;8:e14326 pubmed publisher

image

image 1 :

Western blot analysis of Rat DRG lysate: - 1. Guinea pig Anti-TRPV1 (VR1) Antibody (#ACC-030-GP), (1:200).2. Guinea pig Anti-TRPV1 (VR1) Antibody preincubated with TRPV1/VR1 Blocking Peptide (#BLP-CC030).Following a broad screen of secondary antibodies, the following were used for these applications: Western blot analysis: #106-035-006 (Jackson ImmunoResearch).

image 2 :

Expression of TRPV1 in rat DRGs - Immunohistochemical staining of TRPV1 in rat dorsal root ganglion (DRG) using Guinea pig Anti-TRPV1 (VR1) Antibody (#ACC-030-GP), (1:200). A. TRPV1 staining (red) appears in some DRG cells (arrows). B. DAPI counterstain (blue) is used to stain nuclei. C. Merged image of panels A and B.

product information

CAT :

ACC-030-GP

SKU :

ACC-030-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-TRPV1 (VR1) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O35433

Applications :

IF IHC WB

Reactivity :

Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-CC030

Homology :

Mouse - 14/15 amino acid residues identical; human - 10/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EDAEVFKDSMVPGEK, corresponding to amino acid residues 824-838 of rat TRPV1

Product Page - Scientific background :

TRP channels represent a large and diverse family of non-selective cation channels that respond to a wide range of chemical and physical stimuli. TRPV1 (transient receptor potential vanilloid) is a non-selective cation channel, highly permeable to Ca2+.TRPV1 is comprised of four subunits arranged around a central ion permeation pore. Each subunit consists of six transmembrane α-helices (S1-S6) that span the lipid bilayer and a re-entrant loop with a pore helix located between S5 and S6. The outer pore region of TRPV1 is wide open compared to KV channels, with a broad funnel-like structure that probably enhances accessibility to both small and large pharmacophores.TRPV1 is the receptor for capsaicin, the pungent agent from chili peppers. TRPV1 is activated by noxious heat, elicits burning pain and is modulated by inflammatory agents, such as extracellular protons and bioactive lipids, which contribute to pain hypersensitivity1. TRPV1 activation by capsaicin is followed by nociceptor desensitization, is markedly depending on Ca2+ and involves various intracellular signaling pathways. Subsequently, dephosphorylation by the phosphatase calcineurin of TRPV1 previously phosphorylated by protein kinase A (PKA) or Ca2+-calmodulin-dependent kinase II leads to TRPV1 desensitization2.Recently, the GABA(B) R1 receptor subunit has been identified as an inhibitor of TRPV1 sensitization in the context of diverse inflammatory settings. The endogenous GABA(B) agonist, GABA, is released from nociceptive nerve terminals, suggesting an autocrine feedback mechanism limiting TRPV1 sensitization. The effect of GABA(B) on TRPV1 is independent of canonical G protein signaling and rather relies on close juxtaposition of the GABA(B) R1 receptor subunit and TRPV13.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, OTRPC1

Short Description :

A Guinea Pig Polyclonal Antibody to TRPV1 (VR1) Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat TRPV1 channel. Guinea pig Anti-TRPV1 (VR1) Antibody (#ACC-030-GP, formerly AGP-118), raised in guinea pigs, can be used in western blot analysis. It has been designed to recognize TRPV1 from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-TRPV1 (VR1) Antibody (#ACC-030) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-CC030

Positive Control :

Synonyms :

Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, OTRPC1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPV1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage