antibody

Anti-TRPV1 (VR1)-ATTO Fluor-647N Antibody

ACC-030-FRN

polyclonal

domestic rabbit

nonconjugated

NA

human, mouse, rat

immunohistochemistry, immunocytochemistry

ACC-030-FRN_15 mcl

Anti-TRPV1 (VR1)-ATTO Fluor-647N Antibody

Antibodies

Antibodies

Polyclonal

O35433

IF IHC

Human Rat Mouse

Rabbit

BLP-CC030

Mouse, guinea pig - 14/15 amino acid residues identical; human - 10/15 amino acid residues identical

PBS pH7.4, 1% BSA with 0.05% sodium azide

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

15 µl or 50 µl double distilled water (DDW), depending on the sample size.

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1% BSA, 0.05% NaN3

Intracellular, C-terminus

ATTO-647N. Maximum absorption 646 nm; maximum fluorescence 664 nm. The fluorescence is excited most efficiently in the range 625 - 660 nm. A suitable excitation source is the 647 nm line of the Krypton-Ion laser or a diode-laser emitting at 650 nm. It can be used in flow cytometry (FACS) using the red (637 nm) laser.

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Rat

(C)EDAEVFKDSMVPGEK, corresponding to amino acid residues 824-838 of rat TRPV2

TRPV1 (also named VR1, capsaicin receptor and vanilloid receptor) is a member of the transient receptor potential (TRP) channel family, which includes TRPC, TRPM, TRPA, TRPP, TRPML and the TRPV subfamilies. The TRPV subfamily consists of six members named, TRPV1-6. The TRPV1 channel is a vanilloid gated, nonselective cation channel.The channel has sequence homology to the TRP family, and shares a similar predicted structure of six transmembrane domain (TM) with a pore loop between TM5 and TM6.1 TRPV1 is expressed predominantly in nociceptors and in sensory neurons.2,3TRPV1 has many activators among them heat, protons, vanilloids like capsaicin, resiniferatoxin (RTX), and lipids. This channel is associated with tissue injury and inflammation.5,6Other members of this family, TRPV2, TRPV3 and TRPV4 also show thermal sensitivity.7 Conformational changes in the C-terminus are responsible for many functions such as permeation and gating.There is also evidence that deletion of the C-terminus causes a loss of sensitivity to any stimuli.7 Recent studies demonstrated involvement of TRPV1 in apoptosis where inhibition of the receptor prevented apoptosis.8,9

IF IHC

Alomone Labs

Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, OTRPC2

A Rabbit Polyclonal Antibody to TRPV1 conjugated to the fluorescent dye ATTO Fluor-647N

Anti-TRPV1 (VR1) Antibody (#ACC-030) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize TRPV1 from mouse, rat, and human samples. Anti-TRPV1 (VR1)-ATTO Fluor-647N Antibody (#ACC-030-FRN) is directly conjugated to the ATTO Fluor-647N fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as multicolor immunohistochemistry and immunocytochemistry. It has been designed to recognize TRPV1 from mouse, rat, and human samples.  ATTO Fluor 647N belongs to a new generation of fluorescent labels for the red spectral region. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high thermal and photo-stability, and exceptionally high stability towards atmospheric ozone. Thus ATTO Fluor 647N is highly suitable for single-molecule detection applications and super-resolution microscopy techniques such as Structured Illumination Microscopy (SIM), Stimulated Emission Depletion (STED), etc.

RIC-001-FRN

NA

Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, OTRPC1

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

TRPV1

Lyophilized powder

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis (unlabeled antibody, #ACC-030), and immunohistochemistry (labeled antibody).

1 µg peptide per 1 µg antibody