Anti-TRPV1 (VR1) (extracellular) Antibody | Alomone Labs ACC-029 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TRPV1 (VR1) (extracellular) Antibody

catalog :

ACC-029

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 12

Reference
Cheng K, Wang H, Tseng K, Wang Y, Chang C, Chen Y, et al. Cilostazol Ameliorates Peripheral Neuropathic Pain in Streptozotocin-Induced Type I Diabetic Rats. Front Pharmacol. 2021;12:771271 pubmed publisher
Jiao H, Ivanusic J, McMenamin P, Chinnery H. Distribution of Corneal TRPV1 and Its Association With Immune Cells During Homeostasis and Injury. Invest Ophthalmol Vis Sci. 2021;62:6 pubmed publisher
Zheng Y, Jia C, Jiang X, Chen J, Chen X, Ying X, et al. Electroacupuncture effects on the P2X4R pathway in microglia regulating the excitability of neurons in the substantia gelatinosa region of rats with spinal nerve ligation. Mol Med Rep. 2021;23: pubmed publisher
Miura N, Aung N, Yamakawa M. Effect of cold and hot compress on neutrophilic migration to the site of doxorubicin extravasation. Int J Clin Exp Pathol. 2019;12:1468-1477 pubmed
Ikarashi N, Mizukami N, Kon R, Kaneko M, Uchino R, Fujisawa I, et al. Study of the Mechanism Underlying the Onset of Diabetic Xeroderma Focusing on an Aquaporin-3 in a Streptozotocin-Induced Diabetic Mouse Model. Int J Mol Sci. 2019;20: pubmed publisher
Cun Jin S, Jian Hao X, Xu L, Feng Lun Z, Jie P, Ai Ming S, et al. X-ray induces mechanical and heat allodynia in mouse via TRPA1 and TRPV1 activation. Mol Pain. 2019;15:1744806919849201 pubmed publisher
Kline D, Wang S, Kunze D. TRPV1 channels contribute to spontaneous glutamate release in nucleus tractus solitarii following chronic intermittent hypoxia. J Neurophysiol. 2019;121:881-892 pubmed publisher
Wang W, Ma X, Luo L, Huang M, Dong J, Zhang X, et al. Exchange factor directly activated by cAMP-PKCε signalling mediates chronic morphine-induced expression of purine P2X3 receptor in rat dorsal root ganglia. Br J Pharmacol. 2018;175:1760-1769 pubmed publisher
Ortíz Rentería M, Juárez Contreras R, González Ramírez R, Islas L, Sierra Ramírez F, Llorente I, et al. TRPV1 channels and the progesterone receptor Sig-1R interact to regulate pain. Proc Natl Acad Sci U S A. 2018;115:E1657-E1666 pubmed publisher
Leo M, Schulte M, Schmitt L, Sch xe4 fers M, Kleinschnitz C, Hagenacker T. Intrathecal Resiniferatoxin Modulates TRPV1 in DRG Neurons and Reduces TNF-Induced Pain-Related Behavior. Mediators Inflamm. 2017;2017:2786427 pubmed publisher
Yang J, Cai W, Lu X, Liu S, Zhao S. RNA-Sequencing Analyses Demonstrate the Involvement of Canonical Transient Receptor Potential Channels in Rat Tooth Germ Development. Front Physiol. 2017;8:455 pubmed publisher
Meng J, Wang J, Steinhoff M, Dolly J. TNFÎ± induces co-trafficking of TRPV1/TRPA1 in VAMP1-containing vesicles to the plasmalemma via Munc18-1/syntaxin1/SNAP-25 mediated fusion. Sci Rep. 2016;6:21226 pubmed publisher

image

image 1 :

Western blot analysis of TRPV1 in rat DRG: - 1. Anti-Rat TRPV1 (VR1) (extracellular) Antibody (#ACC-029), (1:200).2. Anti-Rat TRPV1 (VR1) (extracellular) Antibody, preincubated with Rat TRPV1/VR1 (extracellular) Blocking Peptide (#BLP-CC029).

image 2 :

Expression of TRPV1 in rat DRG - Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-Rat TRPV1 (VR1) (extracellular) Antibody (#ACC-029), (1:100) followed by goat anti-rabbit-ATTO Fluor-594 secondary antibody (red). TRPV1 is expressed in medium and small DRG neurons. Hoechst 33342 is used as the counterstain (blue).

product information

CAT :

ACC-029

SKU :

ACC-029-CF_0.2 ml

Product Name :

Anti-TRPV1 (VR1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O35433

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC029

Homology :

Rat - 14 out of 15 amino acid residues identical Mouse - 12 out of 15 amino acid residues identicalWon't recognize TRPV1 from human samples

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)NSLPMESTPHK*SRGS, corresponding to amino acid residues 605-619 of rat TRPV1 with replacement of cysteine 616 (C616) with serine (*S)

Product Page - Scientific background :

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5TRPV1 channel has many activators among them heat, protons, vanilloids like capsaicin, reiniferatoxin (RTX), and lipids. This channel is associated with tissue injury and inflammation.6,7TRPV1 is expressed predominantly in nociceptors and in sensory neurons.Recent studies demonstrated involvement of TRPV1 in apoptosis where inhibition of the receptor prevented apoptosis.8,9

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, OTRPC1

Short Description :

A Rabbit Polyclonal Antibody to Rat TRPV1 (VR1) Channel

Long Description :

Anti-TRPV1 (VR1) (extracellular) Antibody (#ACC-029) is a highly specific antibody directed against an extracellular epitope of the rat TRPV1 channel. The antibody can be used in western blot, immunohistochemistry, and flow cytometry applications. It has been designed to recognize TRPV1 from rat and mouse samples. The antibody will not work with human samples.

Negative Control :

BLP-CC029

Positive Control :

Synonyms :

Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, OTRPC1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPV1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage