Anti-TRPV1 (VR1) (extracellular)-ATTO Fluor-488 Antibody | Alomone Labs ACC-029-AG product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TRPV1 (VR1) (extracellular)-ATTO Fluor-488 Antibody

catalog :

ACC-029-AG

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 488

clone name :

reactivity :

mouse, rat

application :

immunohistochemistry, immunocytochemistry, flow cytometry

more info or order :

Alomone Labs product webpage

citations: 6

Reference
Grelet S, Fr xe9 reux C, Obellianne C, Noguchi K, Howley B, Dalton A, et al. TGFβ-induced expression of long noncoding lincRNA Platr18 controls breast cancer axonogenesis. Life Sci Alliance. 2022;5: pubmed publisher
Nakazawa Y, Petrova R, Sugiyama Y, Nagai N, Tamura H, Donaldson P. Regulation of the Membrane Trafficking of the Mechanosensitive Ion Channels TRPV1 and TRPV4 by Zonular Tension, Osmotic Stress and Activators in the Mouse Lens. Int J Mol Sci. 2021;22: pubmed publisher
Li J, Chen Y, Liu J, Zhang D, Liang P, Lu P, et al. Elevated Expression and Activity of Sodium Leak Channel Contributes to Neuronal Sensitization of Inflammatory Pain in Rats. Front Mol Neurosci. 2021;14:723395 pubmed publisher
Nakazawa Y, Donaldson P, Petrova R. Verification and spatial mapping of TRPV1 and TRPV4 expression in the embryonic and adult mouse lens. Exp Eye Res. 2019;:107707 pubmed publisher
O Leary V, O Connell M, Antyborzec I, Ntziachristos V, Oliver Dolly J, Ovsepian S. Alleviation of Trigeminal Nociception Using p75 Neurotrophin Receptor Targeted Lentiviral Interference Therapy. Neurotherapeutics. 2018;15:489-499 pubmed publisher
Hiyama T, Utsunomiya A, Matsumoto M, Fujikawa A, Lin C, Hara K, et al. Adipsic hypernatremia without hypothalamic lesions accompanied by autoantibodies to subfornical organ. Brain Pathol. 2017;27:323-331 pubmed publisher

image

image 1 :

Multiplex staining of TRPV1 and NaV1.8 in rat DRG - Immunohistochemical staining of rat dorsal root ganglion (DRG) using Anti-Rat TRPV1 (VR1) (extracellular)-ATTO Fluor-488 Antibody (#ACC-029-AG), (green), (1:60) and Anti-NaV1.8 (SCN10A)-ATTO Fluor-594 Antibody (#ASC-016-AR), (red), (1:60). A. TRPV1 staining. B. NaV1.8 staining. C. Merge of A and B demonstrates partial co-localization of TRPV1 and NaV1.8 channels. Nuclei stained using DAPI as the counterstain (blue).

image 2 :

Immuno-colocalization of TRPV1 and NaV1.8 in rat DRG - Immunohistochemical staining of rat dorsal root ganglion (DRG) usingAnti-Rat TRPV1 (VR1) (extracellular)-ATTO-488Antibody (#ACC-029-AG) (green) (1:60) andAnti-NaV1.8 (SCN10A)-ATTO-594 Antibody(#ASC-016-AR) (red) (1:60). A. TRPV1 staining. B. NaV1.8 staining. C. Merge of A and B demonstrates partial co-localization of TRPV1 and NaV1.8 channels. Nuclei stained using DAPI as the counterstain (blue).

product information

CAT :

ACC-029-AG

SKU :

ACC-029-AG_50 mcl

Product Name :

Anti-TRPV1 (VR1) (extracellular)-ATTO Fluor-488 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O35433

Applications :

FC IC IF IHC LCI

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC029

Homology :

Rat - 14 out of 15 amino acid residues identical Mouse - 12 out of 15 amino acid residues identicalWon't recognize TRPV1 from human samples

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

3rd extracellular loop

Label :

ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)NSLPMESTPHK*SRGS, corresponding to amino acid residues 605-619 of rat TRPV1 with replacement of cysteine 616 (C616) with serine (*S)

Product Page - Scientific background :

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1According to IUPHAR the TRP family comprises three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5TRPV1 channel has many activators; among them heat, protons, vanilloids like capsaicin, resiniferatoxin (RTX), and lipids. This channel is associated with tissue injury and inflammation.6,7 TRPV1 is expressed predominantly in nociceptors and in sensory neurons.Recent studies demonstrated involvement of TRPV1 in apoptosis where inhibition of the receptor prevented apoptosis.8,9

Applications may also work in :

FC IC IF IHC LCI

Supplier :

Alomone Labs

Target :

Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, OTRPC1

Short Description :

A Rabbit Polyclonal Antibody to Rat TRPV1 (VR1) Channel Conjugated to the Fluorescent Dye ATTO-488

Long Description :

Anti-TRPV1 (VR1) (extracellular) Antibody (#ACC-029) is a highly specific antibody directed against an extracellular epitope of the rat TRPV1 channel. The antibody can be used in western blot, immunohistochemistry, and live cell imaging applications. It has been designed to recognize TRPV1 from rat and mouse samples. The antibody will not work with human samples. Anti-TRPV1 (VR1) (extracellular)-ATTO Fluor-488 Antibody (#ACC-029-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-TRPV1 (VR1) (extracellular)-ATTO Fluor-488 Antibody has been tested in immunohistochemistry and live cell imaging applications and is especially suited for experiments requiring simultaneous labeling of different markers.

Negative Control :

RIC-001-AG

Positive Control :

Synonyms :

Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, OTRPC1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPV1

AB Specifictiy :

Recognizes TRPV1 from rat only.

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ACC-029), and immunohistochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:50-1:60

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage