Anti-Human TRPV6 (extracellular) Antibody | Alomone Labs ACC-028 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Human TRPV6 (extracellular) Antibody

catalog :

ACC-028

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Bobkov D, Yudintceva N, Lomert E, Shatrova A, Kever L, Semenova S. Lipid raft integrity is required for human leukemia Jurkat T-cell migratory activity. Biochim Biophys Acta Mol Cell Biol Lipids. 2021;1866:158917 pubmed publisher
Crowley L, Cambuli F, Aparicio L, Shibata M, Robinson B, Xuan S, et al. A single-cell atlas of the mouse and human prostate reveals heterogeneity and conservation of epithelial progenitors. elife. 2020;9: pubmed publisher
Kever L, Cherezova A, Zenin V, Negulyaev Y, Komissarchik Y, Semenova S. Downregulation of TRPV6 channel activity by cholesterol depletion in Jurkat T cell line. Cell Biol Int. 2019;43:965-975 pubmed publisher

image

image 1 :

Western blot analysis of human prostate carcinoma cell lines (LNCaP (lanes 1 and 4), DU145 (lanes 2 and 5), PC-3 (lanes 3 and 6)) lysates: - 1-3. Anti-Human TRPV6 (extracellular) Antibody (#ACC-028), (1:200).4-6. Anti-Human TRPV6 (extracellular) Antibody, preincubated with Human TRPV6 (extracellular) Blocking Peptide (#BLP-CC028).

product information

CAT :

ACC-028

SKU :

ACC-028-CF_0.2 ml

Product Name :

Anti-Human TRPV6 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9H1D0

Applications :

IC IF LCI WB

Reactivity :

Human

Host :

Rabbit

Blocking Peptide :

BLP-CC028

Homology :

TRPV6 from human samples The antibody is unlikely to recognize the channel from rat and mouse samples In addition, there is a slight possibility, if any, for some cross-reactivity with human TRPV5

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)RTNNRTSPRDN, corresponding to amino acid residues 395-405 of human TRPV6

Product Page - Scientific background :

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5TRPV6 (CaT1) and TRPV5 (ECaC) are unique members of the TRP family. Both are epithelial calcium channels, form constitutively open channels, share high degree of homology (about 66%) with differences in the N and C-terminus. TRPV6 and TRPV5 are expressed in a 1,25-dihydroxyvitamin D3 dependent manner. In contrast to other members of the TRP family, TRPV6 and TRPV5 are highly Ca2+ selective and are expressed in Ca2+ transporting epithelia where they are assumed to have an important role in Ca2+ reabsorption. TRPV6 is highly expressed in the placenta, small intestine and kidney.6,7While not being expressed in benign prostate tissue, TRPV6 was found to be upregulated in prostate cancer tissue and correlation between expression and tumor grade was shown. Finally, TRPV6 was suggested as prognostic marker and as a promising candidate for therapeutic strategies.8,9

Applications may also work in :

IC IF LCI WB

Supplier :

Alomone Labs

Target :

Epithelial calcium channel 2, ECaC2, Calcium transport protein 1, CaT1, CaT-like, CaT-L, Transient receptor potential cation channel subfamily V member 6

Short Description :

A Rabbit Polyclonal Antibody to Human TRPV6 Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the human TRPV6 channel. Anti-Human TRPV6 (extracellular) Antibody (#ACC-028) can be used in western blot and live cell imaging applications. It has been designed to recognize TRPV6 from human samples. The antibody is unlikely to recognize the channel from rat and mouse samples. In addition, there is a slight possibility, if any, for some cross-reactivity with human TRPV5.

Negative Control :

BLP-CC028

Positive Control :

Synonyms :

Epithelial calcium channel 2, ECaC2, Calcium transport protein 1, CaT1, CaT-like, CaT-L, Transient receptor potential cation channel subfamily V member 6

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPV6

AB Specifictiy :

Not recommended for use in mouse and rat samples. Slight possibility, if any, for some cross reactivity with human TRPV5.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage