Anti-TRPC2 Antibody | Alomone Labs ACC-027 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TRPC2 Antibody

catalog :

ACC-027

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain lysate: - 1. Anti-TRPC2 Antibody (#ACC-027), (1:200).2. Anti-TRPC2 Antibody, preincubated with TRPC2 Blocking Peptide (#BLP-CC027).

image 2 :

Peptide (C)KNRSGVRMFKDGD corresponding to amino acid residues 12-24 of mouse TRPC2 (AccessionQ9R244). Intracellular N-terminus.

image 3 :

Peptide (C)ERRGQGH(S)HRYLLE corresponding to amino acid residues 83-96 of rat OCT3 (Accession O88446). Extracellular 1st loop.

product information

CAT :

ACC-027

SKU :

ACC-027-CF_0.2 ml

Product Name :

Anti-TRPC2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9R244

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC027

Homology :

Rat - 12/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)KNRSGVRMFKDGD, corresponding to amino acid residues 12-24 of mouse TRPC2

Product Page - Scientific background :

TRPC (transient receptor potential cation) channels are the closest mammalian homologs of Drosophila TRP and TRP-like channels. TRPCs are rather nonselective Ca2+ permeable cation channels and affect cell functions through their ability to mediate Ca2+ entry into cells and their action to collapse the plasma membrane potentials1.The mammalian genome codes for seven TRPCs of which TRPC2 is the largest with the most restricted pattern of expression and has several alternatively spliced variant2. Expressed in model cells, TRPC2 mediates both receptor- and store depletion-triggered Ca2+ 3. In human, TPRC2 is a pseudogene3.Physiological roles for TRPC2 have been studied in mature sperm and the vomeronasal sensory system. In sperm, TRPC2 is activated by the sperm's interaction with the oocyte's zona pellucida, leading to entry of Ca2+ and activation of the acrosome reaction4. In the vomeronasal sensory organ (VNO), TRPC2 was found to constitute the transduction channel activated through signaling cascade initiated by the interaction of pheromones with V1R and V2R G protein-coupled receptors on the dendrites of the sensory neurons5. TRPC2-deficient mice display abnormal mating behavior, consistent with a role for this channel in pheromone signaling6.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Short transient receptor potential channel 2, Transient receptor protein 2, TRP2, mTrp2

Short Description :

A Rabbit Polyclonal Antibody to TRPC2 Channel

Long Description :

Anti-TRPC2 Antibody (#ACC-027) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize TRPC2 from rat and mouse samples.

Negative Control :

BLP-CC027

Positive Control :

Synonyms :

Short transient receptor potential channel 2, Transient receptor protein 2, TRP2, mTrp2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPC2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage