product summary
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company name :
Alomone Labs
product type :
antibody
product name :
Anti-CaV3.2 (CACNA1H) Antibody
catalog :
ACC-025
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
NA
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 28
Published Application/Species/Sample/Dilution | Reference |
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image
image 1 :

Western blot analysis of rat DRG lysates: - 1. Anti-CaV3.2 (CACNA1H) Antibody (#ACC-025), (1:200).2. Anti-CaV3.2 (CACNA1H) Antibody, preincubated with Cav3.2/CACNA1H Blocking Peptide (#BLP-CC025).
image 2 :

Expression of CaV3.2 in mouse cerebellum - Immunohistochemical staining of mouse cerebellum frozen sections with Anti-CaV3.2 (CACNA1H) Antibody (#ACC-025), (1:100). A. CaV3.2 appears adjacent to Purkinje cells and in fibers in the molecular layer (red). B. Staining of Purkinje cells with mouse anti-parvalbumin (PV, green). C. Merged image of panels A and B demonstrates presence of CaV3.2 adjacent to Purkinje cells.
image 3 :

Expression of CaV3.2 in mouse cerebellum - Immunohistochemical staining of mouse cerebellum frozen sections withAnti-CaV3.2(CACNA1H)Antibody (#ACC-025) (1:100). A. CaV3.2 appears adjacent to Purkinje cells and in fibers in the molecular layer (red). B. Staining of Purkinje cells with mouse anti-parvalbumin (PV green). C. Merged image of panels A and B demonstrates presence of CaV3.2 adjacent to Purkinjecells.
product information
CAT :
ACC-025
SKU :
ACC-025-CF_0.2 ml
Product Name :
Anti-CaV3.2 (CACNA1H) Antibody
Group Type :
Antibodies
Product Type :
Antibodies
Clonality :
Polyclonal
Accession :
Q9EQ60
Applications :
IC IF IHC IP WB
Reactivity :
Human Rat Mouse
Host :
Rabbit
Blocking Peptide :
BLP-CC025
Homology :
Mouse, bovine and canis - 14/15 amino acid residues identical; human - 13/15 amino acid residues identical
Formulation :
PBS pH7.4
isotype :
Rabbit IgG
Peptide confirmation :
Confirmed by amino acid analysis and mass spectrometry
Reconstitution :
0.2 ml double distilled water (DDW).
Antibody Concentration After Reconstitut ... :
1 mg/ml
Storage After Reconstitution :
The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preservative :
No Preservative
Immunogen Location :
Intracellular loop between domains D1 and D2
Label :
Unconjugated
Storage Before Reconstitution :
The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
Shipping and storage :
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
immunogen source species :
Rat
Sequence :
CHVEGPQERARVAHS, corresponding to amino acid residues 581-595 of rat CaV3.2
Product Page - Scientific background :
T-type Ca2+ channels play an important role in many cellular processes such as hormone secretion, neurotransmitter release and cell differentiation. T-type channels are also known to participate in the pacemaker activities of the heart and neurons including thalamic neurons.1Three genes encoding T-type Ca2+ channels have been cloned and designated as CaV3.2 (CACNA1H), CaV3.1 (CACNA1G) and CaV3.3 (CACNA1I).1-3 The CaV3.2 channel is widely expressed in various tissues such as the brain, heart, liver and testis.Involvement of CaV3.2 Ca2+ channels in several pathologies has been described. Overexpression of the CaV3.2 channel was described in prostate cancer cells that are associated with more aggressiveness, invasiveness and poor prognosis.4 Several point mutations discovered in the CaV3.2 channel that affect gating of the channel were found to be associated with Childhood Absence Epilepsy.5 One year old mice, deficient in CaV3.2 channel, exhibited severe cardiac pathology, fibrosis, necrosis, lymphocyte infiltration and abnormal coronary function compared to wild-type mice.6 Recently, it has been demonstrated that T-type channels are expressed in DRG neurons and that small and medium-diameter primary afferent neurons in the dorsal horn expresses almost exclusively the CaV3.2 Ca2+channels. This might indicate a possible role for CaV3.2 in nociception.7
Applications may also work in :
IC IF IHC IP WB
Supplier :
Alomone Labs
Target :
Voltage-dependent T-type calcium channel subunit α1H
Short Description :
A Rabbit Polyclonal Antibody to CaV3.2 (CACNA1H) Channel
Long Description :
Anti-CaV3.2 (CACNA1H) Antibody (#ACC-025) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize CaV3.2 from rat, human, and mouse samples.
Negative Control :
BLP-CC025
Positive Control :
NA
Synonyms :
Voltage-dependent T-type calcium channel subunit α1H
Lead Time :
1-2 Business Days
Country of origin :
Israel/IL
Applications key :
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Specifictiy :
CACNA1H
Form :
Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Comment :
Contact Alomone Labs for technical support and product customization
Species reactivity key :
H- Human, M- Mouse, R- Rat
Is Toxin :
No
Purity :
Affinity purified on immobilized antigen.
UNSPSC :
41116161
KO-Validated :
yes
Cited Application :
IP IHC ICC
Clone :
NA
Standard quality control of each lot :
Western blot analysis
Antigen preadsorption control :
1 µg peptide per 1 µg antibody
Application Dilutions: Immunohistochemis ... :
1:50-1:100
Application Dilutions: Western blot wb :
1:200
more info or order :
company information

Alomone Labs
Jerusalem BioPark (JBP), Hadassah Ein Kerem
P.O. Box 4287
Jerusalem 9104201
P.O. Box 4287
Jerusalem 9104201
info@alomone.com
http://www.alomone.com972 2 531 8002
headquarters: Israel
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