Anti-CaV3.2 (CACNA1H) Antibody | Alomone Labs ACC-025 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CaV3.2 (CACNA1H) Antibody

catalog :

ACC-025

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Alomone Labs product webpage

citations: 28

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; rat; 1:500; loading ...; fig 4a	Shin S, Cai Y, Itson Zoske B, Qiu C, Hao X, Xiang H, et al. Enhanced T-type calcium channel 3.2 activity in sensory neurons contributes to neuropathic-like pain of monosodium iodoacetate-induced knee osteoarthritis. Mol Pain. 2020;16:1744806920963807 pubmed publisher
immunohistochemistry - frozen section; mouse; loading ...; fig 4b	Fan G, Kaßmann M, Cui Y, Matthaeus C, Kunz S, Zhong C, et al. Age attenuates the T-type CaV 3.2-RyR axis in vascular smooth muscle. Aging Cell. 2020;19:e13134 pubmed publisher
western blot; mouse; 1:200; loading ...; fig 3g	Carrillo Reid L, Day M, Xie Z, Melendez A, Kondapalli J, Plotkin J, et al. Mutant huntingtin enhances activation of dendritic Kv4 K+ channels in striatal spiny projection neurons. elife. 2019;8: pubmed publisher
	Contreras E, Bhoi J, Sonoda T, Birnbaumer L, Schmidt T. Melanopsin activates divergent phototransduction pathways in intrinsically photosensitive retinal ganglion cell subtypes. elife. 2023;12: pubmed publisher
	Eickhoff A, Tjaden J, Stahlke S, Vorgerd M, Theis V, Matschke V, et al. Effects of progesterone on T-type-Ca2+-channel expression in Purkinje cells. Neural Regen Res. 2022;17:2465-2471 pubmed publisher
	Steinecke A, Bolton M, Taniguchi H. Neuromodulatory control of inhibitory network arborization in the developing postnatal neocortex. Sci Adv. 2022;8:eabe7192 pubmed publisher
	Cheng Y, Chiu Y, Dai Z, Wu B. Loganin Ameliorates Painful Diabetic Neuropathy by Modulating Oxidative Stress, Inflammation and Insulin Sensitivity in Streptozotocin-Nicotinamide-Induced Diabetic Rats. Cells. 2021;10: pubmed publisher
	Tjaden J, Eickhoff A, Stahlke S, Gehmeyr J, Vorgerd M, Theis V, et al. Expression Pattern of T-Type Ca2+ Channels in Cerebellar Purkinje Cells after VEGF Treatment. Cells. 2021;10: pubmed publisher
	Liu X, Zhao R, Ding Q, Yao X, Tsang S. TRPC7 regulates the electrophysiological functions of embryonic stem cell-derived cardiomyocytes. Stem Cell Res Ther. 2021;12:262 pubmed publisher
	Ito J, Minemura T, W xe4 lchli S, Niimi T, Fujihara Y, Kuroda S, et al. Id2 Represses Aldosterone-Stimulated Cardiac T-Type Calcium Channels Expression. Int J Mol Sci. 2021;22: pubmed publisher
	Lillo M, Gaete P, Puebla M, Burboa P, Poblete I, Figueroa X. Novel Pannexin-1-Coupled Signaling Cascade Involved in the Control of Endothelial Cell Function and NO-Dependent Relaxation. Oxid Med Cell Longev. 2021;2021:2678134 pubmed publisher
	Sun H, Zhang H, Ross A, Wang T, Al Chami A, Wu S. Developmentally Regulated Rebound Depolarization Enhances Spike Timing Precision in Auditory Midbrain Neurons. Front Cell Neurosci. 2020;14:236 pubmed publisher
	Li B, He X, Dong Z, Xuan K, Sun W, Gao L, et al. Ionomycin ameliorates hypophosphatasia via rescuing alkaline phosphatase deficiency-mediated L-type Ca2+ channel internalization in mesenchymal stem cells. Bone Res. 2020;8:19 pubmed publisher
	Ardestani G, Mehregan A, Fleig A, Horgen F, Carvacho I, Fissore R. Divalent cation influx and calcium homeostasis in germinal vesicle mouse oocytes. Cell Calcium. 2020;87:102181 pubmed publisher
	Zhang B, Chen L, Bai Y, Song J, Cheng J, Ma H, et al. miR-137 and its target T-type CaV 3.1 channel modulate dedifferentiation and proliferation of cerebrovascular smooth muscle cells in simulated microgravity rats by regulating calcineurin/NFAT pathway. Cell Prolif. 2020;53:e12774 pubmed publisher
	Yang T, He M, Zhang H, Barrett P, Hu C. L- and T-type calcium channels control aldosterone production from human adrenals. J Endocrinol. 2020;244:237-247 pubmed publisher
	Cheng X, Ma L, Feng X, Zhu M, Zhang D, Xu L, et al. Antigen retrieval pre-treatment causes a different expression pattern of Cav3.2 in rat and mouse spinal dorsal horn. Eur J Histochem. 2019;63: pubmed publisher
	Joksimovic S, Joksimovic S, Tesic V, Garcia Caballero A, Feseha S, Zamponi G, et al. Selective inhibition of CaV3.2 channels reverses hyperexcitability of peripheral nociceptors and alleviates postsurgical pain. Sci Signal. 2018;11: pubmed publisher
	Kang X, Chi Y, Chen W, Liu F, Cui S, Liao F, et al. Increased expression of CaV3.2 T-type calcium channels in damaged DRG neurons contributes to neuropathic pain in rats with spared nerve injury. Mol Pain. 2018;14:1744806918765808 pubmed publisher
	Chen W, Chi Y, Kang X, Liu Q, Zhang H, Li Z, et al. Accumulation of Cav3.2 T-type Calcium Channels in the Uninjured Sural Nerve Contributes to Neuropathic Pain in Rats with Spared Nerve Injury. Front Mol Neurosci. 2018;11:24 pubmed publisher
	Dong L, Cheng X, Zhou L, Hu Y. Calcium channels are involved in EphB/ephrinB reverse signaling?induced apoptosis in a rat chronic ocular hypertension model. Mol Med Rep. 2017;: pubmed publisher
	Proft J, Rzhepetskyy Y, Lazniewska J, Zhang F, Cain S, Snutch T, et al. The Cacna1h mutation in the GAERS model of absence epilepsy enhances T-type Ca2+ currents by altering calnexin-dependent trafficking of Cav3.2 channels. Sci Rep. 2017;7:11513 pubmed publisher
	Fan J, Gandini M, Zhang F, Chen L, Souza I, Zamponi G. Down-regulation of T-type Cav3.2 channels by hyperpolarization-activated cyclic nucleotide-gated channel 1 (HCN1): Evidence of a signaling complex. Channels (Austin). 2017;11:434-443 pubmed publisher
	Gilbert G, Courtois A, Dubois M, Cussac L, Ducret T, Lory P, et al. T-type voltage gated calcium channels are involved in endothelium-dependent relaxation of mice pulmonary artery. Biochem Pharmacol. 2017;138:61-72 pubmed publisher
	Reimer E, Walenda G, Seidel E, Scholl U. CACNA1H(M1549V) Mutant Calcium Channel Causes Autonomous Aldosterone Production in HAC15 Cells and Is Inhibited by Mibefradil. Endocrinology. 2016;157:3016-22 pubmed publisher
	Liu Z, Wang F, Fischer G, Hogan Q, Yu H. Peripheral nerve injury induces loss of nociceptive neuron-specific G?i-interacting protein in neuropathic pain rat. Mol Pain. 2016;12: pubmed publisher
	Huang D, Liang C, Zhang F, Men H, Du X, Gamper N, et al. Inflammatory mediator bradykinin increases population of sensory neurons expressing functional T-type Ca(2+) channels. Biochem Biophys Res Commun. 2016;473:396-402 pubmed publisher
	Makarenko V, Ahmmed G, Peng Y, Khan S, Nanduri J, Kumar G, et al. CaV3.2 T-type Ca2+ channels mediate the augmented calcium influx in carotid body glomus cells by chronic intermittent hypoxia. J Neurophysiol. 2016;115:345-54 pubmed publisher

image

image 1 :

Western blot analysis of rat DRG lysates: - 1. Anti-CaV3.2 (CACNA1H) Antibody (#ACC-025), (1:200).2. Anti-CaV3.2 (CACNA1H) Antibody, preincubated with Cav3.2/CACNA1H Blocking Peptide (#BLP-CC025).

image 2 :

Expression of CaV3.2 in mouse cerebellum - Immunohistochemical staining of mouse cerebellum frozen sections with Anti-CaV3.2 (CACNA1H) Antibody (#ACC-025), (1:100). A. CaV3.2 appears adjacent to Purkinje cells and in fibers in the molecular layer (red). B. Staining of Purkinje cells with mouse anti-parvalbumin (PV, green). C. Merged image of panels A and B demonstrates presence of CaV3.2 adjacent to Purkinje cells.

image 3 :

Expression of CaV3.2 in mouse cerebellum - Immunohistochemical staining of mouse cerebellum frozen sections withAnti-CaV3.2(CACNA1H)Antibody (#ACC-025) (1:100). A. CaV3.2 appears adjacent to Purkinje cells and in fibers in the molecular layer (red). B. Staining of Purkinje cells with mouse anti-parvalbumin (PV green). C. Merged image of panels A and B demonstrates presence of CaV3.2 adjacent to Purkinjecells.

product information

CAT :

ACC-025

SKU :

ACC-025-CF_0.2 ml

Product Name :

Anti-CaV3.2 (CACNA1H) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9EQ60

Applications :

IC IF IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC025

Homology :

Mouse, bovine and canis - 14/15 amino acid residues identical; human - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular loop between domains D1 and D2

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CHVEGPQERARVAHS, corresponding to amino acid residues 581-595 of rat CaV3.2

Product Page - Scientific background :

T-type Ca2+ channels play an important role in many cellular processes such as hormone secretion, neurotransmitter release and cell differentiation. T-type channels are also known to participate in the pacemaker activities of the heart and neurons including thalamic neurons.1Three genes encoding T-type Ca2+ channels have been cloned and designated as CaV3.2 (CACNA1H), CaV3.1 (CACNA1G) and CaV3.3 (CACNA1I).1-3 The CaV3.2 channel is widely expressed in various tissues such as the brain, heart, liver and testis.Involvement of CaV3.2 Ca2+ channels in several pathologies has been described. Overexpression of the CaV3.2 channel was described in prostate cancer cells that are associated with more aggressiveness, invasiveness and poor prognosis.4 Several point mutations discovered in the CaV3.2 channel that affect gating of the channel were found to be associated with Childhood Absence Epilepsy.5 One year old mice, deficient in CaV3.2 channel, exhibited severe cardiac pathology, fibrosis, necrosis, lymphocyte infiltration and abnormal coronary function compared to wild-type mice.6 Recently, it has been demonstrated that T-type channels are expressed in DRG neurons and that small and medium-diameter primary afferent neurons in the dorsal horn expresses almost exclusively the CaV3.2 Ca2+channels. This might indicate a possible role for CaV3.2 in nociception.7

Applications may also work in :

IC IF IHC IP WB

Supplier :

Alomone Labs

Target :

Voltage-dependent T-type calcium channel subunit α1H

Short Description :

A Rabbit Polyclonal Antibody to CaV3.2 (CACNA1H) Channel

Long Description :

Anti-CaV3.2 (CACNA1H) Antibody (#ACC-025) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize CaV3.2 from rat, human, and mouse samples.

Negative Control :

BLP-CC025

Positive Control :

Synonyms :

Voltage-dependent T-type calcium channel subunit α1H

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CACNA1H

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:50-1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage