Anti-Human CaV1.2 (CACNA1C) Antibody | Alomone Labs ACC-022 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Human CaV1.2 (CACNA1C) Antibody

catalog :

ACC-022

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 25

Reference
Fang Y, Li Q, Li X, Luo G, Kuang S, Luo X, et al. Piezo1 Participated in Decreased L-Type Calcium Current Induced by High Hydrostatic Pressure via. CaM/Src/Pitx2 Activation in Atrial Myocytes. Front Cardiovasc Med. 2022;9:842885 pubmed publisher
Wang C, Horigane S, Wakamori M, Ueda S, Kawabata T, Fujii H, et al. Identification of ultra-rare disruptive variants in voltage-gated calcium channel-encoding genes in Japanese samples of schizophrenia and autism spectrum disorder. Transl Psychiatry. 2022;12:84 pubmed publisher
Alarcón Martínez L, Shiga Y, Villafranca Baughman D, Belforte N, Quintero H, Dotigny F, et al. Pericyte dysfunction and loss of interpericyte tunneling nanotubes promote neurovascular deficits in glaucoma. Proc Natl Acad Sci U S A. 2022;119: pubmed publisher
Lotteau S, Zhang R, Hazan A, Grabar C, Gonzalez D, Aynaszyan S, et al. Acute Genetic Ablation of Cardiac Sodium/Calcium Exchange in Adult Mice: Implications for Cardiomyocyte Calcium Regulation, Cardioprotection, and Arrhythmia. J Am Heart Assoc. 2021;10:e019273 pubmed publisher
Wu Q, Bai Y, Li W, Congdon E, Liu W, Lin Y, et al. Increased neuronal activity in motor cortex reveals prominent calcium dyshomeostasis in tauopathy mice. Neurobiol Dis. 2021;147:105165 pubmed publisher
Li J, Agvanian S, Zhou K, Shaw R, Hong T. Exogenous Cardiac Bridging Integrator 1 Benefits Mouse Hearts With Pre-existing Pressure Overload-Induced Heart Failure. Front Physiol. 2020;11:708 pubmed publisher
Yang H, Lin F, Yang H, Yu P, Huang S, Chen Y, et al. The effect of Sirt1 deficiency on Ca2+ and Na+ regulation in mouse ventricular myocytes. J Cell Mol Med. 2020;24:6762-6772 pubmed publisher
Li J, Wang S, Zhang J, Liu Y, Zheng X, Ding F, et al. The CaMKII phosphorylation site Thr1604 in the CaV1.2 channel is involved in pathological myocardial hypertrophy in rats. Channels (Austin). 2020;14:151-162 pubmed publisher
Zamora N, Cheli V, Santiago González D, Wan R, Paez P. Deletion of Voltage-Gated Calcium Channels in Astrocytes during Demyelination Reduces Brain Inflammation and Promotes Myelin Regeneration in Mice. J Neurosci. 2020;40:3332-3347 pubmed publisher
Morinaga A, Ito J, Niimi T, Maturana A. RBM20 Regulates CaV1.2 Surface Expression by Promoting Exon 9* Inclusion of CACNA1C in Neonatal Rat Cardiomyocytes. Int J Mol Sci. 2019;20: pubmed publisher
Michels S, Dolga A, Braun M, Kisko T, Sungur A, Witt S, et al. Interaction of the Psychiatric Risk Gene Cacna1c With Post-weaning Social Isolation or Environmental Enrichment Does Not Affect Brain Mitochondrial Bioenergetics in Rats. Front Cell Neurosci. 2019;13:483 pubmed publisher
Van Opbergen C, Noorman M, Pfenniger A, Copier J, Vermij S, Li Z, et al. Plakophilin-2 Haploinsufficiency Causes Calcium Handling Deficits and Modulates the Cardiac Response Towards Stress. Int J Mol Sci. 2019;20: pubmed publisher
Chen L, Zhang B, Yang L, Bai Y, Song J, Ge Y, et al. BMAL1 Disrupted Intrinsic Diurnal Oscillation in Rat Cerebrovascular Contractility of Simulated Microgravity Rats by Altering Circadian Regulation of miR-103/CaV1.2 Signal Pathway. Int J Mol Sci. 2019;20: pubmed publisher
Wang X, Zhuo X, Gao J, Liu H, Lin F, Ma A. Neuregulin-1β Partially Improves Cardiac Function in Volume-Overload Heart Failure Through Regulation of Abnormal Calcium Handling. Front Pharmacol. 2019;10:616 pubmed publisher
Schips T, Vanhoutte D, Vo A, Correll R, Brody M, Khalil H, et al. Thrombospondin-3 augments injury-induced cardiomyopathy by intracellular integrin inhibition and sarcolemmal instability. Nat Commun. 2019;10:76 pubmed publisher
Wright P, Sanchez Alonso J, Lucarelli C, Alvarez Laviada A, Poulet C, Bello S, et al. Partial Mechanical Unloading of the Heart Disrupts L-Type Calcium Channel and Beta-Adrenoceptor Signaling Microdomains. Front Physiol. 2018;9:1302 pubmed publisher
Ikeda Y, Zabbarova I, Birder L, Wipf P, Getchell S, Tyagi P, et al. Relaxin-2 therapy reverses radiation-induced fibrosis and restores bladder function in mice. Neurourol Urodyn. 2018;37:2441-2451 pubmed publisher
Michels S, Ganjam G, Martins H, Schratt G, Wohr M, Schwarting R, et al. Downregulation of the psychiatric susceptibility gene Cacna1c promotes mitochondrial resilience to oxidative stress in neuronal cells. Cell Death Discov. 2018;4:54 pubmed publisher
Briot J, Mailhot O, Bourdin B, Tétreault M, Najmanovich R, Parent L. A three-way inter-molecular network accounts for the CaVα2δ1-induced functional modulation of the pore-forming CaV1.2 subunit. J Biol Chem. 2018;293:7176-7188 pubmed publisher
Dong L, Cheng X, Zhou L, Hu Y. Calcium channels are involved in EphB/ephrinB reverse signaling?induced apoptosis in a rat chronic ocular hypertension model. Mol Med Rep. 2017;: pubmed publisher
Cserne Szappanos H, Muralidharan P, Ingley E, Petereit J, Millar A, Hool L. Identification of a novel cAMP dependent protein kinase A phosphorylation site on the human cardiac calcium channel. Sci Rep. 2017;7:15118 pubmed publisher
Okuyama Y, Hirawa N, Fujita M, Fujiwara A, Ehara Y, Yatsu K, et al. The effects of anti-hypertensive drugs and the mechanism of hypertension in vascular smooth muscle cell-specific ATP2B1 knockout mice. Hypertens Res. 2017;: pubmed publisher
Raifman T, Kumar P, Haase H, Klussmann E, Dascal N, Weiss S. Protein kinase C enhances plasma membrane expression of cardiac L-type calcium channel, CaV1.2. Channels (Austin). 2017;11:604-615 pubmed publisher
Bourdin B, Briot J, Tétreault M, Sauve R, Parent L. Negatively charged residues in the first extracellular loop of the L-type CaV1.2 channel anchor the interaction with the CaV?2?1 auxiliary subunit. J Biol Chem. 2017;292:17236-17249 pubmed publisher
Segura E, Bourdin B, Tétreault M, Briot J, Allen B, Mayer G, et al. Proteolytic cleavage of the hydrophobic domain in the CaV?2?1 subunit improves assembly and activity of cardiac CaV1.2 channels. J Biol Chem. 2017;292:11109-11124 pubmed publisher

image

image 1 :

Western blot analysis of rat heart membranes: - 1. Anti-Human-CaV1.2 (CACNA1C) Antibody (#ACC-022), (1:200).2. Anti-Human-CaV1.2 (CACNA1C) Antibody, preincubated with Human Cav1.2/CACNA1C Blocking Peptide (#BLP-CC022).

image 2 :

Peptide VNENTRMYIPEENHQ(C) corresponding to amino acidresidues 2-15 of human CaV1.2 (exon 1B) (AccessionQ13936). Intracellular N-terminus.

product information

CAT :

ACC-022

SKU :

ACC-022-CF_0.2 ml

Product Name :

Anti-Human CaV1.2 (CACNA1C) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q13936

Applications :

IC IF WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC022

Homology :

Mouse, rat - 14/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

VNENTRMYIPEENHQ(C), corresponding to amino acid residues 2-15 of human CaV1.2 (exon 1B)

Product Page - Scientific background :

All L-type calcium channels are encoded by one of the CaV1 channel genes. These channels play a major role as a Ca2+ entry pathway in skeletal, cardiac and smooth muscles as well as in neurons, endocrine cells and possibly in non-excitable cells such as hematopoetic and epithelial cells. All CaV1 channels are influenced by dihydropyridines (DHP) and are also referred to as DHP receptors.While the CaV1.1 and CaV1.4 isoforms are expressed in restricted tissues (skeletal muscle and retina, respectively), the expression of CaV1.2 is ubiquitous and CaV1.3 channels are found in the heart, brain and pancreas. Several peptidyl toxins are described that are specific L-type channel blockers, but so far no selective blocker for one of the CaV1 isoforms have been described. These include the Mamba toxins Calcicludine (#SPC-650), Calciseptine (#C-500) and FS-2 (#F-700).

Applications may also work in :

IC IF WB

Supplier :

Alomone Labs

Target :

Human α1C, Voltage-dependent L-type calcium channel subunit alpha-1C.

Short Description :

A Rabbit Polyclonal Antibody to Human CaV1.2 (CACNA1C) Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human CaV1.2 channel. Anti-Human CaV1.2 (CACNA1C) Antibody (#ACC-022) can be used in western blot and immunocytochemistry applications. It has been designed to recognize CaV1.2 from mouse, rat and human samples.

Negative Control :

BLP-CC022

Positive Control :

Synonyms :

Human α1C, Voltage-dependent L-type calcium channel subunit alpha-1C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CACNA1C

AB Specifictiy :

The antibody recognizes mouse CaV1.2, splice variants P22002-4 and P22002-5 of rat CaV1.2 and splice variant P15381-4 of rabbit CaV1.2.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage