Anti-CACNA1G (CaV3.1) Antibody | Alomone Labs ACC-021 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CACNA1G (CaV3.1) Antibody

catalog :

ACC-021

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 25

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:1000; loading ...; fig 7i	Zhang Y, Qian Z, Jiang D, Sun Y, Gao S, Jiang X, et al. Neuromedin B receptor stimulation of Cav3.2 T-type Ca2+ channels in primary sensory neurons mediates peripheral pain hypersensitivity. Theranostics. 2021;11:9342-9357 pubmed publisher
immunoprecipitation; human; loading ...; fig 7c western blot; human; 1:1000; loading ...; fig 5d	Hu Z, Wang J, Yu D, Soon J, de Kleijn D, Foo R, et al. Aberrant Splicing Promotes Proteasomal Degradation of L-type CaV1.2 Calcium Channels by Competitive Binding for CaVÎ² Subunits in Cardiac Hypertrophy. Sci Rep. 2016;6:35247 pubmed publisher
	Contreras E, Bhoi J, Sonoda T, Birnbaumer L, Schmidt T. Melanopsin activates divergent phototransduction pathways in intrinsically photosensitive retinal ganglion cell subtypes. elife. 2023;12: pubmed publisher
	Eickhoff A, Tjaden J, Stahlke S, Vorgerd M, Theis V, Matschke V, et al. Effects of progesterone on T-type-Ca2+-channel expression in Purkinje cells. Neural Regen Res. 2022;17:2465-2471 pubmed publisher
	Zhang Y, Moran S, Allen A, Baez Nieto D, Xu Q, Wang L, et al. Novel Fluorescence-Based High-Throughput FLIPR Assay Utilizing Membrane-Tethered Genetic Calcium Sensors to Identify T-Type Calcium Channel Modulators. ACS Pharmacol Transl Sci. 2022;5:156-168 pubmed publisher
	Steinecke A, Bolton M, Taniguchi H. Neuromodulatory control of inhibitory network arborization in the developing postnatal neocortex. Sci Adv. 2022;8:eabe7192 pubmed publisher
	Tjaden J, Eickhoff A, Stahlke S, Gehmeyr J, Vorgerd M, Theis V, et al. Expression Pattern of T-Type Ca2+ Channels in Cerebellar Purkinje Cells after VEGF Treatment. Cells. 2021;10: pubmed publisher
	Liu X, Zhao R, Ding Q, Yao X, Tsang S. TRPC7 regulates the electrophysiological functions of embryonic stem cell-derived cardiomyocytes. Stem Cell Res Ther. 2021;12:262 pubmed publisher
	Pathe Neusch xe4 fer Rube A, Neusch xe4 fer Rube F, P xfc schel G. Cell-Based Reporter Release Assay to Determine the Activity of Calcium-Dependent Neurotoxins and Neuroactive Pharmaceuticals. Toxins (Basel). 2021;13: pubmed publisher
	Ito J, Minemura T, W xe4 lchli S, Niimi T, Fujihara Y, Kuroda S, et al. Id2 Represses Aldosterone-Stimulated Cardiac T-Type Calcium Channels Expression. Int J Mol Sci. 2021;22: pubmed publisher
	Mett A, Karbat I, Tsoory M, Fine S, Iwanir S, Reuveny E. Reduced activity of GIRK1-containing heterotetramers is sufficient to affect neuronal functions, including synaptic plasticity and spatial learning and memory. J Physiol. 2021;599:521-545 pubmed publisher
	Sun H, Zhang H, Ross A, Wang T, Al Chami A, Wu S. Developmentally Regulated Rebound Depolarization Enhances Spike Timing Precision in Auditory Midbrain Neurons. Front Cell Neurosci. 2020;14:236 pubmed publisher
	Zhang B, Chen L, Bai Y, Song J, Cheng J, Ma H, et al. miR-137 and its target T-type CaV 3.1 channel modulate dedifferentiation and proliferation of cerebrovascular smooth muscle cells in simulated microgravity rats by regulating calcineurin/NFAT pathway. Cell Prolif. 2020;53:e12774 pubmed publisher
	Miao Q, Herlitze S, Mark M, Noebels J. Adult loss of Cacna1a in mice recapitulates childhood absence epilepsy by distinct thalamic bursting mechanisms. Brain. 2020;143:161-174 pubmed publisher
	Janakiraman U, Yu J, Moutal A, Chinnasamy D, Boinon L, Batchelor S, et al. TAF1-gene editing alters the morphology and function of the cerebellum and cerebral cortex. Neurobiol Dis. 2019;132:104539 pubmed publisher
	Cheng X, Ma L, Feng X, Zhu M, Zhang D, Xu L, et al. Antigen retrieval pre-treatment causes a different expression pattern of Cav3.2 in rat and mouse spinal dorsal horn. Eur J Histochem. 2019;63: pubmed publisher
	Jung H, Kim S, Kim M, Hong J, Yu D, Kim J, et al. Noninvasive optical activation of Flp recombinase for genetic manipulation in deep mouse brain regions. Nat Commun. 2019;10:314 pubmed publisher
	Dong L, Cheng X, Zhou L, Hu Y. Calcium channels are involved in EphB/ephrinB reverse signaling?induced apoptosis in a rat chronic ocular hypertension model. Mol Med Rep. 2017;: pubmed publisher
	Ha S, Lee M, Kurahashi M, Wei L, Jorgensen B, Park C, et al. Transcriptome analysis of PDGFRÎ±+ cells identifies T-type Ca2+ channel CACNA1G as a new pathological marker for PDGFRÎ±+ cell hyperplasia. PLoS ONE. 2017;12:e0182265 pubmed publisher
	Fan J, Gandini M, Zhang F, Chen L, Souza I, Zamponi G. Down-regulation of T-type Cav3.2 channels by hyperpolarization-activated cyclic nucleotide-gated channel 1 (HCN1): Evidence of a signaling complex. Channels (Austin). 2017;11:434-443 pubmed publisher
	Gilbert G, Courtois A, Dubois M, Cussac L, Ducret T, Lory P, et al. T-type voltage gated calcium channels are involved in endothelium-dependent relaxation of mice pulmonary artery. Biochem Pharmacol. 2017;138:61-72 pubmed publisher
	Petersen A, Jensen C, Crepel V, Falkerslev M, Perrier J. Serotonin Regulates the Firing of Principal Cells of the Subiculum by Inhibiting a T-type Ca2+ Current. Front Cell Neurosci. 2017;11:60 pubmed publisher
	Mitani K, Sekiguchi F, Maeda T, Tanaka Y, Yoshida S, Kawabata A. The prostaglandin E2/EP4 receptor/cyclic AMP/T-type Ca(2+) channel pathway mediates neuritogenesis in sensory neuron-like ND7/23 cells. J Pharmacol Sci. 2016;130:177-80 pubmed publisher
	Walton R, Jones S, Rostron K, Kayani A, Close G, McArdle A, et al. Interactions of Short-Term and Chronic Treadmill Training With Aging of the Left Ventricle of the Heart. J Gerontol A Biol Sci Med Sci. 2016;71:1005-13 pubmed publisher
	Díaz Lezama N, Hernández Elvira M, Sandoval A, Monroy A, Felix R, Monjaraz E. Ghrelin inhibits proliferation and increases T-type Ca2+ channel expression in PC-3 human prostate carcinoma cells. Biochem Biophys Res Commun. 2010;403:24-9 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-CACNA1G (CaV3.1) Antibody (#ACC-021), (1:200). 2. Anti-CACNA1G (CaV3.1) Antibody, preincubated with CACNA1G/Cav3.1 Blocking Peptide (#BLP-CC021).

image 2 :

Expression of CACNA1G in rat cerebellum - Immunohistochemical staining of rat cerebellum using Anti-CACNA1G (CaV3.1) Antibody (#ACC-021). A. CACNA1G immunoreactivity (green) appears in the molecular layer. B. Nuclear staining using DAPI as the counterstain (blue). C. Merged images A and B. Mol = molecular layer.

image 3 :

Expression of CACNA1Gin rat cerebellum - Immunohistochemical staining of rat cerebellum usingAnti-CACNA1G (CaV3.1)Antibody (#ACC-021). A. CACNA1Gimmunoreactivity (green) appears in the molecular layer. B. Nuclear staining using DAPI as the counterstain (blue). C. Merged images A and B. Mol = molecular layer.

product information

CAT :

ACC-021

SKU :

ACC-021-CF_0.2 ml

Product Name :

Anti-CACNA1G (CaV3.1) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O54898

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC021

Homology :

Mouse - identical; human - 20/22 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

MDEEEDGAGAEESGQPRSFTQL(C), corresponding to amino acid residues 1-22 of rat CACNA1G

Product Page - Scientific background :

Voltage-dependent Ca2+ channels provide a pathway for rapid influx of Ca2+ into cells, which plays a crucial role in both electrical and metabolic signaling.1 T-type currents are transduced via channel proteins encoded by three genes that compose a subfamily within the CaV channel family.2-3 The activity of T-type channels contributes to several known physiological and pathophysiological phenomena including burst firing in neurons, pacemaking activity in the heart and secretion from endocrine tissues.2 There are three cloned T-type channel isoforms. CACNA1G (CaV3.1) and CACNA1H (CaV3.2) are widely distributed whereas the expression of CACNA1I (CaV3.3) is restricted to the central nervous system.2CACNA1G and CACNA1H are also expressed in the kidney, but little is known about their physiological role there.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Voltage-dependent T-type calcium channel subunit α1G

Short Description :

A Rabbit Polyclonal Antibody to CACNA1G (CaV3.1) Channel

Long Description :

Anti-CACNA1G (CaV3.1) Antibody (#ACC-021) is a highly specific antibody directed against an epitope of the rat CaV3.1 channel. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize CACNA1G from human, rat, and mouse samples.

Negative Control :

BLP-CC021

Positive Control :

Synonyms :

Voltage-dependent T-type calcium channel subunit α1G

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CACNA1G

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage