Anti-CACNA1G (CaV3.1)-ATTO Fluor-594 Antibody | Alomone Labs ACC-021-AR product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CACNA1G (CaV3.1)-ATTO Fluor-594 Antibody

catalog :

ACC-021-AR

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 594

clone name :

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Forster L, Jansen L, Rubaharan M, Murphy A, Baro D. Alterations in SUMOylation of the hyperpolarization-activated cyclic nucleotide-gated ion channel 2 during persistent inflammation. Eur J Pain. 2020;24:1517-1536 pubmed publisher

image

image 1 :

Expression of CACNA1G in rat and mouse cerebellum - Immunohistochemical staining of mouse (A) and rat (B) cerebellum using Anti-CACNA1G (CaV3.1)-ATTO Fluor-594 Antibody (#ACC-021-AR). A, B. CACNA1G staining (red) appears in Purkinje neurons (arrow) and in the molecular layer (Mol). Nuclei were stained using DAPI as the counterstain (Blue).

image 2 :

Alomone Labs SR 48692 inhibits human NTS1 receptors expressed in CHO-K1 cells. - Dose-response curve of hNTSR1 normalized inhibition by SR 48692 (#S-265). Cells were loaded with Fluo-8 dye incubated with increasing concentrations of SR 48692 and stimulated with 3 nM neurotensin. Changes in intracellular Ca2+following agonist application were detected as changes in maximum relative fluorescence (RLU) using FLIPRTETRA?. IC50was calculated at 0.52 M.

product information

CAT :

ACC-021-AR

SKU :

ACC-021-AR_50 mcl

Product Name :

Anti-CACNA1G (CaV3.1)-ATTO Fluor-594 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O54898

Applications :

IF IHC

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC021

Homology :

Mouse - identical; human - 20/22 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Intracellular, N-terminus

Label :

ATTO-594. Maximum absorption 601 nm; Maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

MDEEEDGAGAEESGQPRSFTQL(C), corresponding to amino acid residues 1-22 of rat CACNA1G

Product Page - Scientific background :

Voltage-dependent Ca2+ channels provide a pathway for rapid influx of Ca2+ into cells, which plays a crucial role in both electrical and metabolic signaling.1 T-type currents are transduced via channel proteins encoded by three genes that compose a subfamily within the CaV channel family.2-3 The activity of T-type channels contributes to several known physiological and pathophysiological phenomena including burst firing in neurons, pacemaking activity in the heart and secretion from endocrine tissues.2 There are three cloned T-type channel isoforms. CACNA1G (CaV3.1) and CACNA1H (CaV3.2) are widely distributed whereas the expression of CACNA1I (CaV3.3) is restricted to the central nervous system.2CACNA1G and CACNA1H are also expressed in the kidney, but little is known about their physiological role there.

Applications may also work in :

IF IHC

Supplier :

Alomone Labs

Target :

Voltage-dependent T-type calcium channel subunit α1G

Short Description :

A Rabbit Polyclonal Antibody to CACNA1G (CaV3.1) Conjugated to the Fluorescent Dye ATTO-594

Long Description :

Anti-CACNA1G (CaV3.1) Antibody (#ACC-021) is a highly specific antibody directed against an epitope of the rat CaV3.1 channel. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize CACNA1G from human, rat, and mouse samples. Anti-CACNA1G (CaV3.1)-ATTO Fluor-594 Antibody (#ACC-021-AR) is directly labeled with ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-CACNA1G (CaV3.1)-ATTO Fluor-594 Antibody is ideal for multiplex staining studies.

Negative Control :

BLP-CC021

Positive Control :

Synonyms :

Voltage-dependent T-type calcium channel subunit α1G

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CACNA1G

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ACC-021), and immunohistochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage