Anti-TRPC5 Antibody | Alomone Labs ACC-020 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TRPC5 Antibody

catalog :

ACC-020

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 18

Reference
Perissinotti P, Mart xed nez Hern xe1 ndez E, He Y, Koob M, Piedras Renter xed a E. Genetic Deletion of KLHL1 Leads to Hyperexcitability in Hypothalamic POMC Neurons and Lack of Electrical Responses to Leptin. Front Neurosci. 2021;15:718464 pubmed publisher
Zou Y, Liu Z, Zhou Y, Wang J, Xu Q, Zhao X, et al. TRPC5 mediates TMZ resistance in TMZ-resistant glioblastoma cells via NFATc3-P-gp pathway. Transl Oncol. 2021;14:101214 pubmed publisher
de Sousa Valente J, Alawi K, Keringer P, Bharde S, Ayaz F, Saleque N, et al. Examining the role of transient receptor potential canonical 5 (TRPC5) in osteoarthritis. Osteoarthr Cartil Open. 2020;2:100119 pubmed publisher
Yu Y, Liang Q, Du L, Jiang H, Gu J, Hu H, et al. Synthesis and Characterization of a Specific Iodine-125-Labeled TRPC5 Radioligand. ChemMedChem. 2020;15:1854-1860 pubmed publisher
Guo J, Li J, Xia L, Wang Y, Zhu J, Du J, et al. Transient Receptor Potential Canonical 5-Scramblase Signaling Complex Mediates Neuronal Phosphatidylserine Externalization and Apoptosis. Cells. 2020;9: pubmed publisher
Zou Y, Chen M, Zhang S, Miao Z, Wang J, Lu X, et al. TRPC5‑induced autophagy promotes the TMZ‑resistance of glioma cells via the CAMMKβ/AMPKα/mTOR pathway. Oncol Rep. 2019;41:3413-3423 pubmed publisher
Zhu Y, Gao M, Zhou T, Xie M, Mao A, Feng L, et al. The TRPC5 channel regulates angiogenesis and promotes recovery from ischemic injury in mice. J Biol Chem. 2019;294:28-37 pubmed publisher
Dominguez Rodriguez A, Mayoral Gonzalez I, Ávila Médina J, de Rojas de Pedro E, Calderón Sanchez E, Diaz I, et al. Urocortin-2 Prevents Dysregulation of Ca2+ Homeostasis and Improves Early Cardiac Remodeling After Ischemia and Reperfusion. Front Physiol. 2018;9:813 pubmed publisher
Sunggip C, Shimoda K, Oda S, Tanaka T, Nishiyama K, Mangmool S, et al. TRPC5-eNOS Axis Negatively Regulates ATP-Induced Cardiomyocyte Hypertrophy. Front Pharmacol. 2018;9:523 pubmed publisher
Kim E, Yazdizadeh Shotorbani P, Dryer S. Trpc6 inactivation confers protection in a model of severe nephrosis in rats. J Mol Med (Berl). 2018;96:631-644 pubmed publisher
Wang T, Ning K, Sun X, Zhang C, Jin L, Hua D. Glycolysis is essential for chemoresistance induced by transient receptor potential channel C5 in colorectal cancer. BMC Cancer. 2018;18:207 pubmed publisher
Kim E, Roshanravan H, Dryer S. Changes in podocyte TRPC channels evoked by plasma and sera from patients with recurrent FSGS and by putative glomerular permeability factors. Biochim Biophys Acta Mol Basis Dis. 2017;1863:2342-2354 pubmed publisher
Zhang P, Liu X, Li H, Chen Z, Yao X, Jin J, et al. TRPC5-induced autophagy promotes drug resistance in breast carcinoma via CaMKKβ/AMPKα/mTOR pathway. Sci Rep. 2017;7:3158 pubmed publisher
Obi S, Nakajima T, Hasegawa T, Kikuchi H, Oguri G, Takahashi M, et al. Heat induces interleukin-6 in skeletal muscle cells via TRPV1/PKC/CREB pathways. J Appl Physiol (1985). 2017;122:683-694 pubmed publisher
Chen X, Li W, Riley A, Soliman M, Chakraborty S, Stamatkin C, et al. Molecular Determinants of the Sensitivity to Gq/11-Phospholipase C-dependent Gating, Gd3+ Potentiation, and Ca2+ Permeability in the Transient Receptor Potential Canonical Type 5 (TRPC5) Channel. J Biol Chem. 2017;292:898-911 pubmed publisher
Jiang H, Zeng B, Chen G, Lai B, Lu S, Qu J. Lipopolysaccharide potentiates endothelin-1-induced proliferation of pulmonary arterial smooth muscle cells by upregulating TRPC channels. Biomed Pharmacother. 2016;82:20-7 pubmed publisher
Lau O, Shen B, Wong C, Tjong Y, Lo C, Wang H, et al. TRPC5 channels participate in pressure-sensing in aortic baroreceptors. Nat Commun. 2016;7:11947 pubmed publisher
Sabourin J, Bartoli F, Antigny F, Gomez A, Benitah J. Transient Receptor Potential Canonical (TRPC)/Orai1-dependent Store-operated Ca2+ Channels: NEW TARGETS OF ALDOSTERONE IN CARDIOMYOCYTES. J Biol Chem. 2016;291:13394-409 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-TRPC5 Antibody (#ACC-020), (1:200). 2. Anti-TRPC5 Antibody, preincubated with TRPC5 Blocking Peptide (#BLP-CC020).

image 2 :

Expression of TRPC5 in rat cortex - Immunohistochemical staining of rat cortex using Anti-TRPC5 Antibody (#ACC-020). A. TRPC5 immunoreactivity (green) appears in pyramidal shaped neuronal soma (vertical arrows) and apical dendrites (horizontal arrows). B. Nuclear staining using DAPI as the counterstain (blue). C. Merged image of A and B.

image 3 :

Cell surface detection of P2RY2 in live intact human THP-1 monocytic leukemia cells: - ___Cells.___Cells + rabbit IgG isotype control-FITC.___Cells + Anti-P2Y2 Receptor (extracellular)-FITC Antibody (#APR-102-F) (5 g).

product information

CAT :

ACC-020

SKU :

ACC-020-CF_0.2 ml

Product Name :

Anti-TRPC5 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9UL62

Applications :

IC IF IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC020

Homology :

Rat, mouse, rabbit - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)HKWGDGQEEQVTTRL, corresponding to amino acid residues 959-973 of human TRPC5

Product Page - Scientific background :

The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel families and consists of diverse groups of proteins. In mammals about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels.1-4The TRPC subfamily consists of seven proteins named TRPC1 to 7 which can be further divided into four subgroups based on their sequence homology and functional similarities: 1) TRPC1 2) TRPC4 and TRPC5 3) TRPC3, TRPC6, TRPC7 4) TRPC2.2,5 They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues.TRPC5 channels are assumed to form components of store operated channels in some cell types such as salivary gland cells, endothelial cells and vascular smooth muscle cells, and can be activated either by calcium store depletion or by GPCR stimulation pathways.6

Applications may also work in :

IC IF IHC IP WB

Supplier :

Alomone Labs

Target :

Short transient receptor potential channel 5, Transient receptor protein 5, TRP5

Short Description :

A Rabbit Polyclonal Antibody to TRPC5 Channel

Long Description :

Anti-TRPC5 Antibody (#ACC-020) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize TRPC5 from mouse, rat, and human samples.

Negative Control :

BLP-CC020

Positive Control :

Synonyms :

Short transient receptor potential channel 5, Transient receptor protein 5, TRP5

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPC5

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage