Anti-IP3 Receptor-1 (ITPR1) Antibody | Alomone Labs ACC-019 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-IP3 Receptor-1 (ITPR1) Antibody

catalog :

ACC-019

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Liu X, Zhao R, Ding Q, Yao X, Tsang S. TRPC7 regulates the electrophysiological functions of embryonic stem cell-derived cardiomyocytes. Stem Cell Res Ther. 2021;12:262 pubmed publisher
Radzik T, Boczek T, Ferenc B, Studzian M, Pulaski L, Zylinska L. Calcium Dyshomeostasis Alters CCL5 Signaling in Differentiated PC12 Cells. Biomed Res Int. 2019;2019:9616248 pubmed publisher
Kruse M, Vivas O, TRAYNOR KAPLAN A, Hille B. Dynamics of Phosphoinositide-Dependent Signaling in Sympathetic Neurons. J Neurosci. 2016;36:1386-400 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-IP3 Receptor-1 (ITPR1) Antibody (#ACC-019), (1:2000).2. Anti-IP3 Receptor-1 (ITPR1) Antibody, preincubated with IP3 Receptor-1/ITPR1 Blocking Peptide (#BLP-CC019).

image 2 :

Expression of IP3R1 in mouse cerebellum - Immunohistochemical staining of mouse cerebellum with Anti-IP3 Receptor-1 (ITPR1) Antibody (#ACC-019). Immunoreactivity appears in Purkinje cells (arrows in A) and their dendritic trees. In addition, axonal processes coursing through cerebellar white matter are visualized (arrow in B).

image 3 :

Western blot analysisof rat brain membranes: - 1.Anti-IP3Receptor-1(ITPR1)Antibody (#ACC-019)(1:2000).2. Anti-IP3Receptor-1 (ITPR1) Antibody preincubated with the control peptide antigen.

product information

CAT :

ACC-019

SKU :

ACC-019-CF_0.2 ml

Product Name :

Anti-IP3 Receptor-1 (ITPR1) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P29994

Applications :

IC IF IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC019

Homology :

Human, mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RIGLLGHPPHMNVNPQQPA, corresponding to amino acid residues 2732-2750 of rat IP3R1

Product Page - Scientific background :

Inositol 1,4,5-triphosphate (IP3) serves as a second messenger for many neurotransmitters, hormones and growth factors.1 The binding of IP3 to its receptor (IP3R), which is a ligand-gated Ca2+ channel, located predominantly at the endoplasmic reticulum, results in a rapid release of Ca2+ from intracellular stores.1To date, three known isoforms of IP3R are known (designated IP3R1, IP3R2, and IP3R3) that can work as homotetramers or hetrotetramers. All three receptors have six transmembrane domains and a pore region between TM5 and TM6. The N-terminus as well as the C-terminus face the cytoplasm. Each IP3R consists of an N-terminal ligand binding domain (LBD) and a C-terminal domain which is linked by a long regulatory domain. The C-terminus is constitutively active, suggesting that the regulatory domain is required in order to maintain the suppression of channel activity.2IP3R1 is one of the most important channels responsible for Ca2+ release from intracellular stores and was shown to be the predominant isoform expressed in the central nervous system. IP3R1 is a pivotal player in many neuronal functions, amongst them, neuronal plasticity and neurite extension.3

Applications may also work in :

IC IF IHC IP WB

Supplier :

Alomone Labs

Target :

Inositol 1,4,5-trisphosphate receptor type 1, IP3R1, InsP3R1

Short Description :

A Rabbit Polyclonal Antibody to IP3 Receptor-1

Long Description :

Anti-IP3 Receptor-1 (ITPR1) Antibody (#ACC-019) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize IP3R1 from human, rat, and mouse samples.

Negative Control :

BLP-CC019

Positive Control :

Synonyms :

Inositol 1,4,5-trisphosphate receptor type 1, IP3R1, InsP3R1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ITPR1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:2000

more info or order :

Alomone Labs product webpage