Anti-TRPC4 Antibody | Alomone Labs ACC-018 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TRPC4 Antibody

catalog :

ACC-018

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 15

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:800; fig 5a	Huang M, Lee K, Kim K, Ahn M, Cho C, Kim D, et al. The maintenance ability and Ca2+ availability of skeletal muscle are enhanced by sildenafil. Exp Mol Med. 2016;48:e278 pubmed publisher
	Jeong S, Oh M, Choi J, Woo J, Lee E. Calsequestrin 1 Is an Active Partner of Stromal Interaction Molecule 2 in Skeletal Muscle. Cells. 2021;10: pubmed publisher
	Carver C, DeWitt H, Stoja A, Shapiro M. Blockade of TRPC Channels Limits Cholinergic-Driven Hyperexcitability and Seizure Susceptibility After Traumatic Brain Injury. Front Neurosci. 2021;15:681144 pubmed publisher
	Zhang Y, Yin N, Sun A, Wu Q, Hu W, Hou X, et al. Transient Receptor Potential Channel 6 Knockout Ameliorates Kidney Fibrosis by Inhibition of Epithelial-Mesenchymal Transition. Front Cell Dev Biol. 2020;8:602703 pubmed publisher
	Lee S, Tonello R, Choi Y, Jung S, Berta T. Sensory Neuron-Expressed TRPC4 Is a Target for the Relief of Psoriasiform Itch and Skin Inflammation in Mice. J Invest Dermatol. 2020;140:2221-2229.e6 pubmed publisher
	Guo J, Li J, Xia L, Wang Y, Zhu J, Du J, et al. Transient Receptor Potential Canonical 5-Scramblase Signaling Complex Mediates Neuronal Phosphatidylserine Externalization and Apoptosis. Cells. 2020;9: pubmed publisher
	Kougioumoutzakis A, Pelletier J, Laplante I, Khlaifia A, Lacaille J. TRPC1 mediates slow excitatory synaptic transmission in hippocampal oriens/alveus interneurons. Mol Brain. 2020;13:12 pubmed publisher
	Choi J, Huang M, Hyun C, Oh M, Lee K, Cho C, et al. A muscular hypotonia-associated STIM1 mutant at R429 induces abnormalities in intracellular Ca2+ movement and extracellular Ca2+ entry in skeletal muscle. Sci Rep. 2019;9:19140 pubmed publisher
	Dominguez Rodriguez A, Mayoral Gonzalez I, Ávila Médina J, de Rojas de Pedro E, Calderón Sanchez E, Diaz I, et al. Urocortin-2 Prevents Dysregulation of Ca2+ Homeostasis and Improves Early Cardiac Remodeling After Ischemia and Reperfusion. Front Physiol. 2018;9:813 pubmed publisher
	Griffin C, Thornbury K, Hollywood M, Sergeant G. Muscarinic receptor-induced contractions of the detrusor are impaired in TRPC4 deficient mice. Sci Rep. 2018;8:9264 pubmed publisher
	Dong Q, Liu Q, Li R, Wang A, Bu Q, Wang K, et al. Mechanism and consequence of abnormal calcium homeostasis in Rett syndrome astrocytes. elife. 2018;7: pubmed publisher
	Hamilton C, Abney K, Vasauskas A, Alexeyev M, Li N, Honkanen R, et al. Serine/threonine phosphatase 5 (PP5C/PPP5C) regulates the ISOC channel through a PP5C-FKBP51 axis. Pulm Circ. 2018;8:2045893217753156 pubmed publisher
	Lu T, Wang X, Chai Q, Sun X, Sieck G, Katusic Z, et al. Role of the endothelial caveolae microdomain in shear stress-mediated coronary vasorelaxation. J Biol Chem. 2017;292:19013-19023 pubmed publisher
	Jiang H, Zeng B, Chen G, Lai B, Lu S, Qu J. Lipopolysaccharide potentiates endothelin-1-induced proliferation of pulmonary arterial smooth muscle cells by upregulating TRPC channels. Biomed Pharmacother. 2016;82:20-7 pubmed publisher
	Sabourin J, Bartoli F, Antigny F, Gomez A, Benitah J. Transient Receptor Potential Canonical (TRPC)/Orai1-dependent Store-operated Ca2+ Channels: NEW TARGETS OF ALDOSTERONE IN CARDIOMYOCYTES. J Biol Chem. 2016;291:13394-409 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-TRPC4 Antibody (#ACC-018), (1:200).2. Anti-TRPC4 Antibody, preincubated with TRPC4 Blocking Peptide (#BLP-CC018).

image 2 :

Expression of TRPC4 in mouse cerebellum - Immunohistochemical staining of mouse cerebellum frozen sections with Anti-TRPC4 Antibody (#ACC-018). A. TRPC4 (red) appears in Purkinje cells (arrows) and in the molecular (Mol) layer. B. Staining with mouse anti-parvalbumin (PV) in the same brain section. C. Confocal merge of TRPC4 and PV demonstrates partial co-localization in the Purkinje and the molecular layers.

image 3 :

Immunoprecipitation of rat brain lysate: - 1. Rat brain lysate.2.Lysate immunoprecipitated withAnti-TRPC4Antibody (#ACC-018) (6 mg).3. Lysate immunoprecipitated with pre-immune rabbit serum.The upper arrow indicates the TRPC4 channel while the lower arrow indicates the IgG heavy chain.Western blot analysiswas performed with Anti-TRPC4 Antibody.

product information

CAT :

ACC-018

SKU :

ACC-018-CF_0.2 ml

Product Name :

Anti-TRPC4 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9QUQ5

Applications :

IC IF IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC018

Homology :

Rat, human - 15/16 amino acid residues identical; bovine - 11/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)KEKHAHEEDSSIDYDL, corresponding to amino acid residues 943-958 of mouse TRPC4

Product Page - Scientific background :

The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel families and consists of diverse groups of proteins. In mammals, about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels.1-4 The TRPC subfamily consists of seven proteins named TRPC1 to 7 which can be further divided into four subgroups based on their sequence homology and functional similarities: 1. TRPC1 2. TRPC4 and TRPC5 3. TRPC3, TRPC6, TRPC7 4. TRPC2.2,5 They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues.TRPC4 can form heterotetramers with TRPC1. TRPC4, TRPC1 and TRPC5 can be activated either by calcium store depletion or by GPCR stimulation pathways and are also assumed to form components of store operated channels in some cell types such as salivary gland cells, endothelial cells and vascular smooth muscle cells. 6

Applications may also work in :

IC IF IHC IP WB

Supplier :

Alomone Labs

Target :

Transient receptor potential channel 4, TRP4, hTrp4, Capacitative calcium entry channel Trp4, CCE1

Short Description :

A Rabbit Polyclonal Antibody to TRPC4 Channel

Long Description :

Anti-TRPC4 Antibody (#ACC-018) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize TRPC4 from human, mouse, and rat samples.

Negative Control :

BLP-CC018

Positive Control :

Synonyms :

Transient receptor potential channel 4, TRP4, hTrp4, Capacitative calcium entry channel Trp4, CCE1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPC4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage