Anti-TRPC6 Antibody | Alomone Labs ACC-017 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TRPC6 Antibody

catalog :

ACC-017

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat, dogs

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Alomone Labs product webpage

citations: 42

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 4b immunocytochemistry; human; loading ...; fig 4e	Chen Q, Zhou Y, Zhou L, Fu Z, Yang C, Zhao L, et al. TRPC6-dependent Ca2+ signaling mediates airway inflammation in response to oxidative stress via ERK pathway. Cell Death Dis. 2020;11:170 pubmed publisher
western blot; mouse; 1:800; fig 5a	Huang M, Lee K, Kim K, Ahn M, Cho C, Kim D, et al. The maintenance ability and Ca2+ availability of skeletal muscle are enhanced by sildenafil. Exp Mol Med. 2016;48:e278 pubmed publisher
immunoprecipitation; dogs; 1:1000; fig 7c western blot; human; 1:1000; fig 10	Hammers D, Sleeper M, Forbes S, Shima A, Walter G, Sweeney H. Tadalafil Treatment Delays the Onset of Cardiomyopathy in Dystrophin-Deficient Hearts. J Am Heart Assoc. 2016;5: pubmed publisher
	Servello D, Abdinghoff J, Grissmer A, Tschernig T. Transient receptor potential channel 6 in human skeletal muscle fibers: Investigation in fresh and conserved tissue samples. Biomed Rep. 2022;17:60 pubmed publisher
	Abdinghoff J, Servello D, Jacobs T, Beckmann A, Tschernig T. Evaluation of the presence of TRPC6 channels in human vessels: A pilot study using immunohistochemistry. Biomed Rep. 2022;16:42 pubmed publisher
	He C, Li Q, Cui Y, Gao P, Shu W, Zhou Q, et al. Recurrent moderate hypoglycemia accelerates the progression of Alzheimer's disease through impairment of the TRPC6/GLUT3 pathway. JCI Insight. 2022;7: pubmed publisher
	Lee T, Chung C, Lee T, Lin Y, Kao Y, Chen Y. Fibroblast Growth Factor 23 Stimulates Cardiac Fibroblast Activity through Phospholipase C-Mediated Calcium Signaling. Int J Mol Sci. 2021;23: pubmed publisher
	Jeong S, Oh M, Choi J, Woo J, Lee E. Calsequestrin 1 Is an Active Partner of Stromal Interaction Molecule 2 in Skeletal Muscle. Cells. 2021;10: pubmed publisher
	t Hart D, Li J, van der Vlag J, Nijenhuis T. Repurposing Riociguat to Target a Novel Paracrine Nitric Oxide-TRPC6 Pathway to Prevent Podocyte Injury. Int J Mol Sci. 2021;22: pubmed publisher
	Hou X, Huang M, Zeng X, Zhang Y, Sun A, Wu Q, et al. The Role of TRPC6 in Renal Ischemia/Reperfusion and Cellular Hypoxia/Reoxygenation Injuries. Front Mol Biosci. 2021;8:698975 pubmed publisher
	Kawade A, Yamamura A, Fujiwara M, Kobayashi S, Mori S, Horii C, et al. Comparative analysis of age in monocrotaline-induced pulmonary hypertensive rats. J Pharmacol Sci. 2021;147:81-85 pubmed publisher
	Golosova D, Palygin O, Bohovyk R, Klemens C, Levchenko V, Spires D, et al. Role of opioid signaling in kidney damage during the development of salt-induced hypertension. Life Sci Alliance. 2020;3: pubmed publisher
	Gu L, Ge H, Zhao L, Wang Y, Zhang F, Tang H, et al. Huangkui Capsule Ameliorates Renal Fibrosis in a Unilateral Ureteral Obstruction Mouse Model Through TRPC6 Dependent Signaling Pathways. Front Pharmacol. 2020;11:996 pubmed publisher
	Mei L, Zheng Y, Song T, Yadav V, Joseph L, Truong L, et al. Rieske iron-sulfur protein induces FKBP12.6/RyR2 complex remodeling and subsequent pulmonary hypertension through NF-κB/cyclin D1 pathway. Nat Commun. 2020;11:3527 pubmed publisher
	Zhang X, Luo D, Bai X, Ding H, Liu M, Deng J, et al. Upregulation of TRPC6 Mediated by PAX6 Hypomethylation Is Involved in the Mechanical Allodynia Induced by Chemotherapeutics in Dorsal Root Ganglion. Int J Neuropsychopharmacol. 2020;23:257-267 pubmed publisher
	Choi J, Huang M, Hyun C, Oh M, Lee K, Cho C, et al. A muscular hypotonia-associated STIM1 mutant at R429 induces abnormalities in intracellular Ca2+ movement and extracellular Ca2+ entry in skeletal muscle. Sci Rep. 2019;9:19140 pubmed publisher
	Yap J, Tai Y, Fröhlich J, Fong C, Yin J, Foo Z, et al. Ambient and supplemental magnetic fields promote myogenesis via a TRPC1-mitochondrial axis: evidence of a magnetic mitohormetic mechanism. FASEB J. 2019;33:12853-12872 pubmed publisher
	Paez Espinosa E, Lin O, Karim Z, Alshbool F, Khasawneh F. Mouse transient receptor potential channel type 6 selectively regulates agonist-induced platelet function. Biochem Biophys Rep. 2019;20:100685 pubmed publisher
	Bernardini M, Brossa A, Chinigo G, Grolez G, Trimaglio G, Allart L, et al. Transient Receptor Potential Channel Expression Signatures in Tumor-Derived Endothelial Cells: Functional Roles in Prostate Cancer Angiogenesis. Cancers (Basel). 2019;11: pubmed publisher
	Numaga Tomita T, Shimauchi T, Oda S, Tanaka T, Nishiyama K, Nishimura A, et al. TRPC6 regulates phenotypic switching of vascular smooth muscle cells through plasma membrane potential-dependent coupling with PTEN. FASEB J. 2019;33:9785-9796 pubmed publisher
	Chen X, Taylor Nguyen N, Riley A, Herring B, White F, Obukhov A. The TRPC6 inhibitor, larixyl acetate, is effective in protecting against traumatic brain injury-induced systemic endothelial dysfunction. J Neuroinflammation. 2019;16:21 pubmed publisher
	Kim E, Hassanzadeh Khayyat N, Dryer S. Mechanisms underlying modulation of podocyte TRPC6 channels by suPAR: Role of NADPH oxidases and Src family tyrosine kinases. Biochim Biophys Acta Mol Basis Dis. 2018;1864:3527-3536 pubmed publisher
	Hou X, Xiao H, Zhang Y, Zeng X, Huang M, Chen X, et al. Transient receptor potential channel 6 knockdown prevents apoptosis of renal tubular epithelial cells upon oxidative stress via autophagy activation. Cell Death Dis. 2018;9:1015 pubmed publisher
	Dominguez Rodriguez A, Mayoral Gonzalez I, Ávila Médina J, de Rojas de Pedro E, Calderón Sanchez E, Diaz I, et al. Urocortin-2 Prevents Dysregulation of Ca2+ Homeostasis and Improves Early Cardiac Remodeling After Ischemia and Reperfusion. Front Physiol. 2018;9:813 pubmed publisher
	Sunggip C, Shimoda K, Oda S, Tanaka T, Nishiyama K, Mangmool S, et al. TRPC5-eNOS Axis Negatively Regulates ATP-Induced Cardiomyocyte Hypertrophy. Front Pharmacol. 2018;9:523 pubmed publisher
	Ilatovskaya D, Blass G, Palygin O, Levchenko V, Pavlov T, Grzybowski M, et al. A NOX4/TRPC6 Pathway in Podocyte Calcium Regulation and Renal Damage in Diabetic Kidney Disease. J Am Soc Nephrol. 2018;29:1917-1927 pubmed publisher
	Kim E, Yazdizadeh Shotorbani P, Dryer S. Trpc6 inactivation confers protection in a model of severe nephrosis in rats. J Mol Med (Berl). 2018;96:631-644 pubmed publisher
	Zhang L, Chen X, Qin H, Jiang L, Qin Y. ATRA attenuate proteinuria via downregulation of TRPC6 in glomerulosclerosis rats induced by adriamycin. Ren Fail. 2018;40:266-272 pubmed publisher
	Oh M, Lee K, Huang M, Kim J, Kim D, Cho C, et al. STIM2 regulates both intracellular Ca2+ distribution and Ca2+ movement in skeletal myotubes. Sci Rep. 2017;7:17936 pubmed publisher
	Ohba T, Watanabe H, Murakami M, Iino K, Adachi T, Baba Y, et al. Stromal interaction molecule 1 haploinsufficiency causes maladaptive response to pressure overload. PLoS ONE. 2017;12:e0187950 pubmed publisher
	Zhang L, Ji T, Wang Q, Meng K, Zhang R, Yang H, et al. Calcium-Sensing Receptor Stimulation in Cultured Glomerular Podocytes Induces TRPC6-Dependent Calcium Entry and RhoA Activation. Cell Physiol Biochem. 2017;43:1777-1789 pubmed publisher
	Hong W, Peng G, Hao B, Liao B, Zhao Z, Zhou Y, et al. Nicotine-Induced Airway Smooth Muscle Cell Proliferation Involves TRPC6-Dependent Calcium Influx Via α7 nAChR. Cell Physiol Biochem. 2017;43:986-1002 pubmed publisher
	Urban N, Neuser S, Hentschel A, Köhling S, Rademann J, Schaefer M. Pharmacological inhibition of focal segmental glomerulosclerosis-related, gain of function mutants of TRPC6 channels by semi-synthetic derivatives of larixol. Br J Pharmacol. 2017;174:4099-4122 pubmed publisher
	Kim E, Roshanravan H, Dryer S. Changes in podocyte TRPC channels evoked by plasma and sera from patients with recurrent FSGS and by putative glomerular permeability factors. Biochim Biophys Acta Mol Basis Dis. 2017;1863:2342-2354 pubmed publisher
	He X, Li S, Liu B, Susperreguy S, Formoso K, Yao J, et al. Major contribution of the 3/6/7 class of TRPC channels to myocardial ischemia/reperfusion and cellular hypoxia/reoxygenation injuries. Proc Natl Acad Sci U S A. 2017;114:E4582-E4591 pubmed publisher
	Liu N, Zhuang Y, Zhou Z, Zhao J, Chen Q, Zheng J. NF-?B dependent up-regulation of TRPC6 by A? in BV-2 microglia cells increases COX-2 expression and contributes to hippocampus neuron damage. Neurosci Lett. 2017;651:1-8 pubmed publisher
	Krauszman A, Mak T, Szaszi K, Kuebler W. Role of phosphatase and tensin homolog in hypoxic pulmonary vasoconstriction. Cardiovasc Res. 2017;113:869-878 pubmed publisher
	Jadeja R, Thounaojam M, Khurana S. Characterization of pressure-mediated vascular tone in resistance arteries from bile duct-ligated rats. Oncotarget. 2017;8:30706-30722 pubmed publisher
	Yang L, Liu B, Lu X, Yan Y, Zhai Y, Bao Q, et al. Inhibition of TRPC6 reduces non-small cell lung cancer cell proliferation and invasion. Oncotarget. 2017;8:5123-5134 pubmed publisher
	Alvarez Miguel I, Cidad P, Pérez García M, Lopez Lopez J. Differences in TRPC3 and TRPC6 channels assembly in mesenteric vascular smooth muscle cells in essential hypertension. J Physiol. 2017;595:1497-1513 pubmed publisher
	Jiang Q, Lu W, Yang K, Hadadi C, Fu X, Chen Y, et al. Sodium tanshinone IIA sulfonate inhibits hypoxia-induced enhancement of SOCE in pulmonary arterial smooth muscle cells via the PKG-PPAR-? signaling axis. Am J Physiol Cell Physiol. 2016;311:C136-49 pubmed publisher
	Urban N, Wang L, Kwiek S, Rademann J, Kuebler W, Schaefer M. Identification and Validation of Larixyl Acetate as a Potent TRPC6 Inhibitor. Mol Pharmacol. 2016;89:197-213 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-TRPC6 Antibody (#ACC-017), (1:200).2. Anti-TRPC6 Antibody, preincubated with TRPC6 Blocking Peptide (#BLP-CC017).

image 2 :

Expression of TRPC6 in mouse cerebellum - Immunohistochemical staining of mouse cerebellum using Anti-TRPC6 Antibody (#ACC-017). A. TRPC6 (red) appears in Purkinje cells (arrows) and in the molecular (Mol) layer. B. Staining with mouse anti parvalbumin in the same brain section. C. Confocal merge of TRPC6 and PV demonstrates partial co-localization in the Purkinje and the molecular layers.

image 3 :

Expression of TRPC6 in human kidney from MN patients - Immunohistochemical staining of human biopsy specimens from MN patients FSGS patients and normal kidney tissue from tumor nephrectomy samples using Anti-TRPC6 Antibody (#ACC-017). TRPC6 expression was below detection levels in control kidneys and only slightly induced in FSGS glomeruli whereas MN kidneys mostly showed moderate to strong positivity for TRPC6.Adapted from Kistler A.D. et al. (2013) J. Biol. Chem. 288 36598. with permission of the American Society for Biochemistry and Molecular Biology.

product information

CAT :

ACC-017

SKU :

ACC-017-CF_0.2 ml

Product Name :

Anti-TRPC6 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q61143

Applications :

IC IF IFC IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC017

Homology :

Rat - identical; human - 15/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)RRNESQDYLLMDELG, corresponding to amino acid residues 24-38 of mouse TRPC6

Product Page - Scientific background :

The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel families and consists of diverse groups of proteins. In mammals, about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels.1-4The TRPC subfamily consists of seven proteins named TRPC1 to 7 which can be further divided into four subgroups based on their sequence homology and functional similarities:1. TRPC1. 2. TRPC4 and TRPC5. 3. TRPC3, TRPC6, TRPC7. 4. TRPC2.2,5They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues.TRPC6 can form heterotetramers with TRPC3 and TRPC7. It is primarily expressed in brain, lung and muscle. High levels of expression of the channel were also found in human platelets. Recently it was reported that TRPC6 is also expressed in the kidney where a mutated channel has been implicated in kidney failure disease.6,7

Applications may also work in :

IC IF IFC IHC IP WB

Supplier :

Alomone Labs

Target :

Short transient receptor potential channel 6, Transient receptor protein 6, TRP6

Short Description :

A Rabbit Polyclonal Antibody to TRPC6 Channel

Long Description :

Anti-TRPC6 Antibody (#ACC-017) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunoprecipitation, indirect flow cytometry, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize TRPC6 from mouse, rat, and human samples.

Negative Control :

BLP-CC017

Positive Control :

Synonyms :

Short transient receptor potential channel 6, Transient receptor protein 6, TRP6

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPC6

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP IHC ICC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage