Guinea pig Anti-TRPC6 Antibody | Alomone Labs ACC-017-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-TRPC6 Antibody

catalog :

ACC-017-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Guinea pig Anti-TRPC6 Antibody (#ACC-017-GP), (1:200).2. Guinea pig Anti-TRPC6 Antibody, preincubated with TRPC6 Blocking Peptide (#BLP-CC017).

image 2 :

Expression of TRPC6 in rat cerebellum - Immunohistochemical staining of rat cerebellum using Guinea pig Anti-TRPC6 Antibody (#ACC-017-GP). A. TRPC6 (green) appears in the soma of Purkinje cells (white arrow). B. Parvalbumin (red), a marker of Purkinje and interneuronal cells, is stained in the same section. C. Merge of the images demonstrates intense expression of TRPC6 in the soma of Purkinje cells and lower expression in the molecular (Mol) layer. DAPI is used as the counterstain (blue).

product information

CAT :

ACC-017-GP

SKU :

ACC-017-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-TRPC6 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q61143

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-CC017

Homology :

Rat - identical; human - 15/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)RRNESQDYLLMDELG, corresponding to amino acid residues 24-38 of mouse TRPC6

Product Page - Scientific background :

Transient receptor potential (TRP) channels are relatively non-selective ion channels enabling the exchange of cations down their electrochemical gradient. This exchange enables the intracellular rise in Na+ and Ca2+ concentration and ultimately in the cell membrane depolarization, important for action potential propagation and muscle contraction1. They are activated by an extremely broad range of stimuli namely, temperature, voltage, pH, endocrine factors as well as signaling molecules2.The TRP channel family is composed of 28 members divided in 7 subgroups: TRPV, TRPC, TRPM, TRPA, TRPN, TRPP and TRPML. All members of the TRP family form tetramers and could heterhomultimerize. They have 6 transmembrane (TM) domains, and a pore domain between the fifth (S5) and sixth (S6) transmembrane domains. In general, TRP channels enable the passage of either Na+ or Ca2+ ions with little or no preference. However, some channels do exhibit some selectivity. Also, TRP channels do not display the positive charges in the transmembrane domain number 4 voltage-sensing domain like most voltage sensitive channels, although they do display voltage dependency3. In addition, TRP channels have in the C-terminal intracellular region to the S6 domain a TRP domain comprising 25 amino acids that is more or less conserved among most TRP channels. Within the TRP domain, there is a TRP box composed of six amino acids, and TRP box 2 - a proline rich domain1,3. The TRP domain seems to be responsible for the binding of PIP2, a phospholipid important for the regulation of channel activity4.The TRPC subfamily is further divided into the following: TRPC1/4/5, TRPC3/6/7 and TRPC21. Activation of phospholipase C (PLC) ultimately leads to the formation of diacylglycerol (DAG) and inositol 1,4,5-triphosphate (IP3) via hydrolysis of PIP2. The increase in concentration of these intracellular second messengers leads to the activation of non-selective Ca2+ channels and an IP3-induced release of Ca2+ from intracellular stores5. The intracellular Ca2+ store depletion in turn activates Ca2+ specific channels to allow replenish intracellular Ca2+ levels. TRPCs are thought to be activated upon intracellular store Ca2+ depletion, and may function in concert with the recently identified Orai channel5. The TRPC3/6/7 class produces similar currents upon activation.TRPC6 can form heterotetramers with TRPC3 and TRPC7. It is primarily expressed in brain, lung and muscle. High levels of expression of the channel were also found in human platelets. Recently it was reported that TRPC6 is also expressed in the kidney where a mutated channel has been implicated in kidney failure disease6,7.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Short transient receptor potential channel 6, Transient receptor protein 6, TRP6

Short Description :

A Guinea Pig Polyclonal Antibody to TRPC6 Channel

Long Description :

Guinea pig Anti-TRPC6 Antibody (#ACC-017-GP, formerly AGP-002), raised in guinea pigs, is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize TRPC6 from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-TRPC6 Antibody (#ACC-017) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-CC017

Positive Control :

Synonyms :

Short transient receptor potential channel 6, Transient receptor protein 6, TRP6

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPC6

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage