Anti-TRPC3 Antibody | Alomone Labs ACC-016 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TRPC3 Antibody

catalog :

ACC-016

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat, dogs

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 39

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:800; fig 5a	Huang M, Lee K, Kim K, Ahn M, Cho C, Kim D, et al. The maintenance ability and Ca2+ availability of skeletal muscle are enhanced by sildenafil. Exp Mol Med. 2016;48:e278 pubmed publisher
immunoprecipitation; dogs; 1:500; fig 7c	Hammers D, Sleeper M, Forbes S, Shima A, Walter G, Sweeney H. Tadalafil Treatment Delays the Onset of Cardiomyopathy in Dystrophin-Deficient Hearts. J Am Heart Assoc. 2016;5: pubmed publisher
	Farfariello V, Gordienko D, Mesilmany L, Touil Y, Germain E, Fliniaux I, et al. TRPC3 shapes the ER-mitochondria Ca2+ transfer characterizing tumour-promoting senescence. Nat Commun. 2022;13:956 pubmed publisher
	Jeong S, Oh M, Choi J, Woo J, Lee E. Calsequestrin 1 Is an Active Partner of Stromal Interaction Molecule 2 in Skeletal Muscle. Cells. 2021;10: pubmed publisher
	Casas J, Meana C, Lopez Lopez J, Balsinde J, Balboa M. Lipin-1-derived diacylglycerol activates intracellular TRPC3 which is critical for inflammatory signaling. Cell Mol Life Sci. 2021;78:8243-8260 pubmed publisher
	Um K, Hahn S, Kim S, Lee Y, Birnbaumer L, Kim H, et al. TRPC3 and NALCN channels drive pacemaking in substantia nigra dopaminergic neurons. elife. 2021;10: pubmed publisher
	Matthews A, Soni H, Robinson Freeman K, John T, Buddington R, Adebiyi A. Doxorubicin-Induced Fetal Mesangial Cell Death Occurs Independently of TRPC6 Channel Upregulation but Involves Mitochondrial Generation of Reactive Oxygen Species. Int J Mol Sci. 2021;22: pubmed publisher
	Azuma Y, Suzuki S, Nishiyama K, Yamaguchi T. Gastrointestinal motility modulation by stress is associated with reduced smooth muscle contraction through specific transient receptor potential channel. J Vet Med Sci. 2021;83:622-629 pubmed publisher
	Bocchero U, Falleroni F, Mortal S, Li Y, Cojoc D, LAMB T, et al. Mechanosensitivity is an essential component of phototransduction in vertebrate rods. PLoS Biol. 2020;18:e3000750 pubmed publisher
	Liu Y, Liu X, Zhang X, Deng J, Zhang J, Xing H. lncRNA DLX6-AS1 Promotes Proliferation of Laryngeal Cancer Cells by Targeting the miR-26a/TRPC3 Pathway. Cancer Manag Res. 2020;12:2685-2695 pubmed publisher
	Li S, Jiang K, Li J, Hao X, Chu W, Luo C, et al. Estrogen enhances the proliferation and migration of ovarian cancer cells by activating transient receptor potential channel C3. J Ovarian Res. 2020;13:20 pubmed publisher
	Cozart M, Phelan K, Wu H, Mu S, Birnbaumer L, Rusch N, et al. Vascular smooth muscle TRPC3 channels facilitate the inverse hemodynamic response during status epilepticus. Sci Rep. 2020;10:812 pubmed publisher
	Urban N, Schaefer M. Direct Activation of TRPC3 Channels by the Antimalarial Agent Artemisinin. Cells. 2020;9: pubmed publisher
	Choi J, Huang M, Hyun C, Oh M, Lee K, Cho C, et al. A muscular hypotonia-associated STIM1 mutant at R429 induces abnormalities in intracellular Ca2+ movement and extracellular Ca2+ entry in skeletal muscle. Sci Rep. 2019;9:19140 pubmed publisher
	Bernardini M, Brossa A, Chinigo G, Grolez G, Trimaglio G, Allart L, et al. Transient Receptor Potential Channel Expression Signatures in Tumor-Derived Endothelial Cells: Functional Roles in Prostate Cancer Angiogenesis. Cancers (Basel). 2019;11: pubmed publisher
	Sudi S, Tanaka T, Oda S, Nishiyama K, Nishimura A, Sunggip C, et al. TRPC3-Nox2 axis mediates nutritional deficiency-induced cardiomyocyte atrophy. Sci Rep. 2019;9:9785 pubmed publisher
	Nishiyama K, Numaga Tomita T, Fujimoto Y, Tanaka T, Toyama C, Nishimura A, et al. Ibudilast attenuates doxorubicin-induced cytotoxicity by suppressing formation of TRPC3 channel and NADPH oxidase 2 protein complexes. Br J Pharmacol. 2019;176:3723-3738 pubmed publisher
	Wang Y, Qi Y, Qi Z, Tsang S. TRPC3 Regulates the Proliferation and Apoptosis Resistance of Triple Negative Breast Cancer Cells through the TRPC3/RASA4/MAPK Pathway. Cancers (Basel). 2019;11: pubmed publisher
	Picardo M, Sugimura Y, Dorst K, Kallurkar P, Akins V, Ma X, et al. Trpm4 ion channels in pre-Bötzinger complex interneurons are essential for breathing motor pattern but not rhythm. PLoS Biol. 2019;17:e2006094 pubmed publisher
	Kim E, Shotorbani P, Dryer S. TRPC6 inactivation does not affect loss of renal function in nephrotoxic serum glomerulonephritis in rats, but reduces severity of glomerular lesions. Biochem Biophys Rep. 2019;17:139-150 pubmed publisher
	Naito R, Kassai H, Sakai Y, Schönherr S, Fukaya M, Schwarzer C, et al. New Features on the Expression and Trafficking of mGluR1 Splice Variants Exposed by Two Novel Mutant Mouse Lines. Front Mol Neurosci. 2018;11:439 pubmed publisher
	Hou X, Xiao H, Zhang Y, Zeng X, Huang M, Chen X, et al. Transient receptor potential channel 6 knockdown prevents apoptosis of renal tubular epithelial cells upon oxidative stress via autophagy activation. Cell Death Dis. 2018;9:1015 pubmed publisher
	Kim E, Yazdizadeh Shotorbani P, Dryer S. Trpc6 inactivation confers protection in a model of severe nephrosis in rats. J Mol Med (Berl). 2018;96:631-644 pubmed publisher
	Koizumi H, John T, Chia J, Tariq M, Phillips R, Mosher B, et al. Transient Receptor Potential Channels TRPM4 and TRPC3 Critically Contribute to Respiratory Motor Pattern Formation but not Rhythmogenesis in Rodent Brainstem Circuits. Eneuro. 2018;5: pubmed publisher
	Liu B, He X, Li S, Xu B, Birnbaumer L, Liao Y. Deletion of diacylglycerol-responsive TRPC genes attenuates diabetic nephropathy by inhibiting activation of the TGF?1 signaling pathway. Am J Transl Res. 2017;9:5619-5630 pubmed
	Shimauchi T, Numaga Tomita T, Ito T, Nishimura A, Matsukane R, Oda S, et al. TRPC3-Nox2 complex mediates doxorubicin-induced myocardial atrophy. JCI Insight. 2017;2: pubmed publisher
	Wang X, Sun W, Fu J, Huang J, Yu C, Underwood M, et al. Impairment of Coronary Endothelial Function by Hypoxia-Reoxygenation Involves TRPC3 Inhibition-mediated KCa Channel Dysfunction: Implication in Ischemia-Reperfusion Injury. Sci Rep. 2017;7:5895 pubmed publisher
	Wang B, Xiong S, Lin S, Xia W, Li Q, Zhao Z, et al. Enhanced Mitochondrial Transient Receptor Potential Channel, Canonical Type 3-Mediated Calcium Handling in the Vasculature From Hypertensive Rats. J Am Heart Assoc. 2017;6: pubmed publisher
	Jia S, Rodriguez M, Williams A, Yuan J. Homer binds to Orai1 and TRPC channels in the neointima and regulates vascular smooth muscle cell migration and proliferation. Sci Rep. 2017;7:5075 pubmed publisher
	Liu N, Zhuang Y, Zhou Z, Zhao J, Chen Q, Zheng J. NF-?B dependent up-regulation of TRPC6 by A? in BV-2 microglia cells increases COX-2 expression and contributes to hippocampus neuron damage. Neurosci Lett. 2017;651:1-8 pubmed publisher
	Sun Y, Zhang H, Selvaraj S, Sukumaran P, Lei S, Birnbaumer L, et al. Inhibition of L-Type Ca2+ Channels by TRPC1-STIM1 Complex Is Essential for the Protection of Dopaminergic Neurons. J Neurosci. 2017;37:3364-3377 pubmed publisher
	Mamenko M, Boukelmoune N, Tomilin V, Zaika O, Jensen V, O Neil R, et al. The renal TRPV4 channel is essential for adaptation to increased dietary potassium. Kidney Int. 2017;91:1398-1409 pubmed publisher
	Chen M, Liu J, Lu Y, Duan C, Lu L, Gao G, et al. Age-dependent alpha-synuclein accumulation is correlated with elevation of mitochondrial TRPC3 in the brains of monkeys and mice. J Neural Transm (Vienna). 2017;124:441-453 pubmed publisher
	Storck H, Hild B, Schimmelpfennig S, Sargin S, Nielsen N, Zaccagnino A, et al. Ion channels in control of pancreatic stellate cell migration. Oncotarget. 2017;8:769-784 pubmed publisher
	Alvarez Miguel I, Cidad P, Pérez García M, Lopez Lopez J. Differences in TRPC3 and TRPC6 channels assembly in mesenteric vascular smooth muscle cells in essential hypertension. J Physiol. 2017;595:1497-1513 pubmed publisher
	Kim J, Xie J, Hwang K, Wu Y, Oliver N, Eom M, et al. Klotho May Ameliorate Proteinuria by Targeting TRPC6 Channels in Podocytes. J Am Soc Nephrol. 2017;28:140-151 pubmed publisher
	Wang Q, Leo M, Narayanan D, Kuruvilla K, Jaggar J. Local coupling of TRPC6 to ANO1/TMEM16A channels in smooth muscle cells amplifies vasoconstriction in cerebral arteries. Am J Physiol Cell Physiol. 2016;310:C1001-9 pubmed publisher
	Sabourin J, Bartoli F, Antigny F, Gomez A, Benitah J. Transient Receptor Potential Canonical (TRPC)/Orai1-dependent Store-operated Ca2+ Channels: NEW TARGETS OF ALDOSTERONE IN CARDIOMYOCYTES. J Biol Chem. 2016;291:13394-409 pubmed publisher
	Ampem P, Smedlund K, Vazquez G. Pharmacological evidence for a role of the transient receptor potential canonical 3 (TRPC3) channel in endoplasmic reticulum stress-induced apoptosis of human coronary artery endothelial cells. Vascul Pharmacol. 2016;76:42-52 pubmed publisher

image

image 1 :

Western blot analysis of rat heart membranes: - 1. Anti-TRPC3 Antibody (#ACC-016), (1:200).2. Anti-TRPC3 Antibody, preincubated with TRPC3 Blocking Peptide (#BLP-CC016).

image 2 :

Expression of TRPC3 in rat pituitary gland - Immunohistochemical staining of rat pituitary gland paraffin embedded sections using Anti-TRPC3 Antibody (#ACC-016), (1:100). TRPC3 is mainly expressed in the adenohypophysis (on left). Hematoxilin is used as the counterstain.

image 3 :

Western blotanalysisof rat heart membranes: - 1.Anti-TRPC3Antibody (#ACC-016) (1:200).2. Anti-TRPC3 Antibody preincubated with the control peptide antigen.

product information

CAT :

ACC-016

SKU :

ACC-016-CF_0.2 ml

Product Name :

Anti-TRPC3 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9QZC1

Applications :

IC IF IFC IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC016

Homology :

Human, rat - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

HKLSEKLNPSVLRC, corresponding to amino acid residues 822-835 of mouse TRPC3

Product Page - Scientific background :

The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel families and consists of diverse groups of proteins. In mammals, about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels.1-4The TRPC subfamily consists of seven proteins named TRPC1 to 7 which can be further divided into four subgroups based on their sequence homology and functional similarities:1. TRPC12. TRPC4 and TRPC53. TRPC3, TRPC6, TRPC74. TRPC2.2,5They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues.TRPC3, TRPC6 and TRPC7 form non-selective cationic channels that are activated by the stimulation of GPCRs.Different modes of activation, DAG and SOC, were described for the TRPC3 channel by different investigators working with heterologous expression systems.6,7

Applications may also work in :

IC IF IFC IHC IP WB

Supplier :

Alomone Labs

Target :

Short transient receptor potential channel 3, Transient receptor protein 3, TRP3

Short Description :

A Rabbit Polyclonal Antibody to TRPC3 Channel

Long Description :

Anti-TRPC3 Antibody (#ACC-016) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, immunocytochemistry, and indirect flow cytometry applications. It has been designed to recognize TRPC3 from mouse, rat, and human samples.

Negative Control :

BLP-CC016

Positive Control :

Synonyms :

Short transient receptor potential channel 3, Transient receptor protein 3, TRP3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPC3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage