Guinea pig Anti-TRPC3 Antibody | Alomone Labs ACC-016-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-TRPC3 Antibody

catalog :

ACC-016-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Yotsumoto T, Maemura K, Watanabe K, Amano Y, Matsumoto Y, Zokumasu K, et al. NRXN1 as a novel potential target of antibody-drug conjugates for small cell lung cancer. Oncotarget. 2020;11:3590-3600 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysate: - 1. Guinea pig Anti-TRPC3 Antibody (#ACC-016-GP), (1:400).2. Guinea pig Anti-TRPC3 Antibody preincubated with TRPC3 Blocking Peptide (#BLP-CC016).

image 2 :

Expression of TRPC3 in mouse cerebellum. - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Guinea pig Anti-TRPC3 Antibody (#ACC-016-GP), (1:200), followed by goat anti-guinea pig-AlexaFluor-594. A. TRPC3 immunoreactivity (red) appears in purkinje cells (arrows). B. Pre-incubation of the antibody with TRPC3 Blocking Peptide (#BLP-CC016), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

ACC-016-GP

SKU :

ACC-016-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-TRPC3 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9QZC1

Applications :

IHC WB

Reactivity :

Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-CC016

Homology :

Human, rat - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

HKLSEKLNPSVLRC, corresponding to amino acid residues 822-835 of mouse TRPC3

Product Page - Scientific background :

The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel families and consists of diverse groups of proteins. In mammals, about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin),TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels.1-4The TRPC subfamily consists of seven proteins named TRPC1 to 7 which can be further divided into four subgroups based on their sequence homology and functional similarities: 1. TRPC1 2. TRPC4 and TRPC5 3. TRPC3, TRPC6, TRPC7 4. TRPC2.2,5 They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues.TRPC3, TRPC6 and TRPC7 form non-selective cationic channels that are activated by the stimulation of GPCRs.Different modes of activation, DAG and SOC, were described for the TRPC3 channel by different investigators working with heterologous expression systems.6,7

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Short transient receptor potential channel 3, Transient receptor protein 3, TRP3

Short Description :

A Guinea Pig Polyclonal Antibody to TRPC3 Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the mouse TRPC3 channel. Guinea pig Anti-TRPC3 Antibody (#ACC-016-GP, formerly AGP-018) raised in guinea pig can be used in western blot analysis. It has been designed to recognize TRPC3 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-TRPC3 Antibody (#ACC-016) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-CC016

Positive Control :

Synonyms :

Short transient receptor potential channel 3, Transient receptor protein 3, TRP3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPC3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage