Anti-TRPC3-ATTO Fluor-594 Antibody | Alomone Labs ACC-016-AR product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TRPC3-ATTO Fluor-594 Antibody

catalog :

ACC-016-AR

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 594

clone name :

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Pentz R, Iulita M, Ducatenzeiler A, Videla L, Benejam B, Carmona Iragui M, et al. Nerve growth factor (NGF) pathway biomarkers in Down syndrome prior to and after the onset of clinical Alzheimer's disease: A paired CSF and plasma study. Alzheimers Dement. 2021;17:605-617 pubmed publisher

image

image 1 :

Expression of TRPC3 in rat DRG - Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-TRPC3-ATTO Fluor-594 Antibody (#ACC-016-AR), (1:50). Staining is present in neuronal cell bodies. Hoechst 33342 is used as the counterstain.

image 2 :

Colocalization of TRPC6 and TRPC3 in rat cerebellum - Immunohistochemical staining of rat cerebellum frozen section usingGuinea pig Anti-TRPC6 Antibody(#AGP-002) and rabbit Anti-TRPC3-ATTO-594Antibody (#ACC-016-AR). A. TRPC6 staining (green) appears in molecular layer and in Purkinje cells. B. In the same section as in A staining of TRPC3 (red) appears as well in both molecular layer and Purkinje cells. C. Merge images of A and B indicates co-localization in Purkinje cells and molecular layer.

image 3 :

Expression of TRPC3 in rat DRG - Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-TRPC3-ATTO-594Antibody (#ACC-016-AR) (1:50). Staining is present in neuronal cell bodies. Hoechst 33342 is used as the counterstain.

product information

CAT :

ACC-016-AR

SKU :

ACC-016-AR_50 mcl

Product Name :

Anti-TRPC3-ATTO Fluor-594 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9QZC1

Applications :

IF IHC

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC016

Homology :

Human, rat - 13/14 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Intracellular, C-terminus

Label :

ATTO-594. Maximum absorption 601 nm; Maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

HKLSEKLNPSVLRC, corresponding to amino acid residues 822-835 of mouse TRPC3

Product Page - Scientific background :

The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel family and consists of diverse groups of proteins. In mammals about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels.1-4The TRPC subfamily consists of seven proteins named TRPC1 to 7 which can be further divided into four subgroups based on their sequence homology and functional similarities: TRPC1; TRPC4 and TRPC5; TRPC3, TRPC6, TRPC7 and TRPC2.2,5 They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues.TRPC3, TRPC6 and TRPC7 form non-selective cationic channels that are activated by the stimulation of GPCRs.TRPC3 was shown to be activated by DAG and SOC, in heterologous expression systems.6,7

Applications may also work in :

IF IHC

Supplier :

Alomone Labs

Target :

Short transient receptor potential channel 3, Transient receptor protein 3, TRP3

Short Description :

A Rabbit Polyclonal Antibody to TRPC3 Channel Conjugated to the Fluorescent Dye ATTO-594

Long Description :

Anti-TRPC3 Antibody (#ACC-016) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, immunocytochemistry, and indirect flow cytometry applications. It has been designed to recognize TRPC3 from mouse, rat, and human samples. Anti-TRPC3-ATTO Fluor-594 Antibody (#ACC-016-AR) is directly labeled with ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-TRPC3-ATTO Fluor-594 Antibody can be used in immunohistochemistry applications and is specially suited to experiments requiring simultaneous labeling of different markers.

Negative Control :

BLP-CC016

Positive Control :

Synonyms :

Short transient receptor potential channel 3, Transient receptor protein 3, TRP3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPC3

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ACC-016), and immunohistochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:50-1:60

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage