Anti-CACNA2D1 (CaVα2δ1) (extracellular) Antibody | Alomone Labs ACC-015 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CACNA2D1 (CaVα2δ1) (extracellular) Antibody

catalog :

ACC-015

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

domestic rabbit

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 15

Reference
Al Katat A, Zhao J, Calderone A, Parent L. Sympathetic Stimulation Upregulates the Ca2+ Channel Subunit, CaVα2δ1, via the β1 and ERK 1/2 Pathway in Neonatal Ventricular Cardiomyocytes. Cells. 2022;11: pubmed publisher
Li L, Chen S, Zhou M, Wang L, Li D, Chen H, et al. α2δ-1 switches the phenotype of synaptic AMPA receptors by physically disrupting heteromeric subunit assembly. Cell Rep. 2021;36:109396 pubmed publisher
Ednie A, Bennett E. Intracellular O-linked glycosylation directly regulates cardiomyocyte L-type Ca2+ channel activity and excitation-contraction coupling. Basic Res Cardiol. 2020;115:59 pubmed publisher
Li B, He X, Dong Z, Xuan K, Sun W, Gao L, et al. Ionomycin ameliorates hypophosphatasia via rescuing alkaline phosphatase deficiency-mediated L-type Ca2+ channel internalization in mesenchymal stem cells. Bone Res. 2020;8:19 pubmed publisher
Korrapati S, Taukulis I, Olszewski R, Pyle M, Gu S, Singh R, et al. Single Cell and Single Nucleus RNA-Seq Reveal Cellular Heterogeneity and Homeostatic Regulatory Networks in Adult Mouse Stria Vascularis. Front Mol Neurosci. 2019;12:316 pubmed publisher
Deng M, Chen S, Chen H, Pan H. α2δ-1-Bound N-Methyl-D-aspartate Receptors Mediate Morphine-induced Hyperalgesia and Analgesic Tolerance by Potentiating Glutamatergic Input in Rodents. Anesthesiology. 2019;130:804-819 pubmed publisher
Chen Y, Chen S, Chen H, Zhang J, Pan H. Increased α2δ-1-NMDA receptor coupling potentiates glutamatergic input to spinal dorsal horn neurons in chemotherapy-induced neuropathic pain. J Neurochem. 2019;148:252-274 pubmed publisher
Ednie A, Deng W, Yip K, Bennett E. Reduced myocyte complex N-glycosylation causes dilated cardiomyopathy. FASEB J. 2019;33:1248-1261 pubmed publisher
Brockhaus J, Schreitmüller M, Repetto D, Klatt O, Reissner C, ELMSLIE K, et al. ?-Neurexins Together with ?2?-1 Auxiliary Subunits Regulate Ca2+ Influx through Cav2.1 Channels. J Neurosci. 2018;38:8277-8294 pubmed publisher
Ma H, Chen S, Chen H, Li L, Li D, Zhou J, et al. α2δ-1 Is Essential for Sympathetic Output and NMDA Receptor Activity Potentiated by Angiotensin II in the Hypothalamus. J Neurosci. 2018;38:6388-6398 pubmed publisher
Koh S, Chen W, Dejneka N, Harris I, Lu B, Girman S, et al. Subretinal Human Umbilical Tissue-Derived Cell Transplantation Preserves Retinal Synaptic Connectivity and Attenuates Müller Glial Reactivity. J Neurosci. 2018;38:2923-2943 pubmed publisher
Bourdin B, Briot J, Tétreault M, Sauve R, Parent L. Negatively charged residues in the first extracellular loop of the L-type CaV1.2 channel anchor the interaction with the CaV?2?1 auxiliary subunit. J Biol Chem. 2017;292:17236-17249 pubmed publisher
Scott M, Kammermeier P. CaV2 channel subtype expression in rat sympathetic neurons is selectively regulated by ?2? subunits. Channels (Austin). 2017;11:555-573 pubmed publisher
Segura E, Bourdin B, Tétreault M, Briot J, Allen B, Mayer G, et al. Proteolytic cleavage of the hydrophobic domain in the CaV?2?1 subunit improves assembly and activity of cardiac CaV1.2 channels. J Biol Chem. 2017;292:11109-11124 pubmed publisher
Tedeschi A, Dupraz S, Laskowski C, Xue J, Ulas T, Beyer M, et al. The Calcium Channel Subunit Alpha2delta2 Suppresses Axon Regeneration in the Adult CNS. Neuron. 2016;92:419-434 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-CACNA2D1 (CaVα2δ1) (extracellular) Antibody (#ACC-015), (1:200).2. Anti-CACNA2D1 (CaVα2δ1) (extracellular) Antibody, preincubated with CACNA2D1/Cavα2δ1 (extracellular) Blocking Peptide (#BLP-CC015).

image 2 :

Expression of CaV?2?1 in rat PC12 cells - Cell surface detection ofCaV?2?1 in intact living rat Pheochromocytoma (PC12) cells. A. Extracellular staining of cells usingAnti-CACNA2D1 (CaV?2?1) (extracellular)Antibody (#ACC-015) (1:50) (red). B. Merge of A with the live view of the cell.

image 3 :

Western blot analysisof rat brain membranes: - 1.Anti-CACNA2D1 (CaV?2?1) (extracellular)Antibody (#ACC-015)(1:200).2. Anti-CACNA2D1 (CaV?2?1) (extracellular) Antibody preincubated with the control peptide antigen.

product information

CAT :

ACC-015

SKU :

ACC-015-CF_0.2 ml

Product Name :

Anti-CACNA2D1 (CaVα2δ1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P13806

Applications :

IC IF LCI WB

Reactivity :

Human Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC015

Homology :

Pig, human - identical; mouse, rat - 14/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rabbit

Sequence :

EPFPSAVTIKSWVDK(C), corresponding to amino acid residues 27-41 of rabbit CaVα2δ1 precursor

Product Page - Scientific background :

The α2δ1 protein, encoded by CACNA2D1, is an auxiliary subunit of the CaV (voltage-dependent calcium) channel multimer complex. Four α2δ isoforms were identified in mammalian genomes. They share some sequence, as well as structure, homology.1The protein product of the gene encoding α2δ is complex; it includes two separated proteins - the extracellular α2 peptide linked by disulfide bridges to the membrane spanning (single transmembrane helix) δ subunit.The α2δ1 subunit is highly expressed in skeletal muscle and brain. Its expression along with a pore forming CaV α1 subunit, result in larger CaV currents, probably due to improved trafficking of the α1 subunit to the plasma membrane.1-2 α2δ1 subunit was shown to be the target of the antiepileptic and analgesic drug Gabapentin.3

Applications may also work in :

IC IF LCI WB

Supplier :

Alomone Labs

Target :

Voltage-dependent Ca2+ channel subunit alpha-2/delta-1, L-type calcium channel alpha 2 polypeptide, Dihydropyridine-sensitive L-type calcium channel alpha-2/delta subunit

Short Description :

A Rabbit Polyclonal Antibody to CACNA2D1 (CaVα2δ1 Subunit)

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rabbit CaVα2δ1. Anti-CACNA2D1 (CaVα2δ1) (extracellular) Antibody (#ACC-015) can be used in western blot and live cell imaging applications. It is especially suited to detect CaVα2δ1 in live cells. It has been designed to recognize CaVα2δ1 from rat, mouse and human samples.

Negative Control :

BLP-CC015

Positive Control :

Synonyms :

Voltage-dependent Ca2+ channel subunit alpha-2/delta-1, L-type calcium channel alpha 2 polypeptide, Dihydropyridine-sensitive L-type calcium channel alpha-2/delta subunit

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CACNA2D1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage