Anti-TRPC1 Antibody | Alomone Labs ACC-010 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TRPC1 Antibody

catalog :

ACC-010

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 35

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:800; fig 5a	Huang M, Lee K, Kim K, Ahn M, Cho C, Kim D, et al. The maintenance ability and Ca2+ availability of skeletal muscle are enhanced by sildenafil. Exp Mol Med. 2016;48:e278 pubmed publisher
	Skobeleva K, Shalygin A, Mikhaylova E, Guzhova I, Ryazantseva M, Kaznacheyeva E. The STIM1/2-Regulated Calcium Homeostasis Is Impaired in Hippocampal Neurons of the 5xFAD Mouse Model of Alzheimer's Disease. Int J Mol Sci. 2022;23: pubmed publisher
	Wang L, Ling D, Huang M, Tao L, Tong Q, Hou Y, et al. Influence of atherosclerosis on the molecular expression of the TRPC1/BK signal complex in the aortic smooth muscles of mice. Exp Ther Med. 2022;23:4 pubmed publisher
	Lee T, Chung C, Lee T, Lin Y, Kao Y, Chen Y. Fibroblast Growth Factor 23 Stimulates Cardiac Fibroblast Activity through Phospholipase C-Mediated Calcium Signaling. Int J Mol Sci. 2021;23: pubmed publisher
	Jeong S, Oh M, Choi J, Woo J, Lee E. Calsequestrin 1 Is an Active Partner of Stromal Interaction Molecule 2 in Skeletal Muscle. Cells. 2021;10: pubmed publisher
	Cabezas Garcia E, Gordon A, Mulligan F, Ferris C. Revisiting the Relationships between Fat-to-Protein Ratio in Milk and Energy Balance in Dairy Cows of Different Parities, and at Different Stages of Lactation. Animals (Basel). 2021;11: pubmed publisher
	Nascimento Da Conceicao V, Sun Y, Ramachandran K, Chauhan A, Raveendran A, Venkatesan M, et al. Resolving macrophage polarization through distinct Ca2+ entry channel that maintains intracellular signaling and mitochondrial bioenergetics. iScience. 2021;24:103339 pubmed publisher
	Carver C, DeWitt H, Stoja A, Shapiro M. Blockade of TRPC Channels Limits Cholinergic-Driven Hyperexcitability and Seizure Susceptibility After Traumatic Brain Injury. Front Neurosci. 2021;15:681144 pubmed publisher
	Zhu J, Zhang X, Xie H, Wang Y, Zhang X, Lin Z. Cardiomyocyte Stim1 Deficiency Exacerbates Doxorubicin Cardiotoxicity by Magnification of Endoplasmic Reticulum Stress. J Inflamm Res. 2021;14:3945-3958 pubmed publisher
	Mei L, Zheng Y, Song T, Yadav V, Joseph L, Truong L, et al. Rieske iron-sulfur protein induces FKBP12.6/RyR2 complex remodeling and subsequent pulmonary hypertension through NF-κB/cyclin D1 pathway. Nat Commun. 2020;11:3527 pubmed publisher
	Bocchero U, Falleroni F, Mortal S, Li Y, Cojoc D, LAMB T, et al. Mechanosensitivity is an essential component of phototransduction in vertebrate rods. PLoS Biol. 2020;18:e3000750 pubmed publisher
	He D, Mao A, Li Y, Tam S, Zheng Y, Yao X, et al. TRPC1 participates in the HSV-1 infection process by facilitating viral entry. Sci Adv. 2020;6:eaaz3367 pubmed publisher
	Kougioumoutzakis A, Pelletier J, Laplante I, Khlaifia A, Lacaille J. TRPC1 mediates slow excitatory synaptic transmission in hippocampal oriens/alveus interneurons. Mol Brain. 2020;13:12 pubmed publisher
	Choi J, Huang M, Hyun C, Oh M, Lee K, Cho C, et al. A muscular hypotonia-associated STIM1 mutant at R429 induces abnormalities in intracellular Ca2+ movement and extracellular Ca2+ entry in skeletal muscle. Sci Rep. 2019;9:19140 pubmed publisher
	Ambite I, Butler D, Stork C, Gr nberg Hern ndez J, K ves B, Zdziarski J, et al. Fimbriae reprogram host gene expression - Divergent effects of P and type 1 fimbriae. PLoS Pathog. 2019;15:e1007671 pubmed publisher
	Debant M, Burgos M, Hemon P, Buscaglia P, Fali T, Melayah S, et al. STIM1 at the plasma membrane as a new target in progressive chronic lymphocytic leukemia. J Immunother Cancer. 2019;7:111 pubmed publisher
	Czeredys M, Vigont V, Boeva V, Mikoshiba K, Kaznacheyeva E, Kuznicki J. Huntingtin-Associated Protein 1A Regulates Store-Operated Calcium Entry in Medium Spiny Neurons From Transgenic YAC128 Mice, a Model of Huntington's Disease. Front Cell Neurosci. 2018;12:381 pubmed publisher
	D Costa S, Ayyadurai S, Gibson A, Mackey E, Rajput M, Sommerville L, et al. Mast cell corticotropin-releasing factor subtype 2 suppresses mast cell degranulation and limits the severity of anaphylaxis and stress-induced intestinal permeability. J Allergy Clin Immunol. 2019;143:1865-1877.e4 pubmed publisher
	Tang L, Yao F, Wang H, Wang X, Shen J, Dai B, et al. Inhibition of TRPC1 prevents cardiac hypertrophy via NF-κB signaling pathway in human pluripotent stem cell-derived cardiomyocytes. J Mol Cell Cardiol. 2019;126:143-154 pubmed publisher
	Dominguez Rodriguez A, Mayoral Gonzalez I, Ávila Médina J, de Rojas de Pedro E, Calderón Sanchez E, Diaz I, et al. Urocortin-2 Prevents Dysregulation of Ca2+ Homeostasis and Improves Early Cardiac Remodeling After Ischemia and Reperfusion. Front Physiol. 2018;9:813 pubmed publisher
	Martinez Galan J, Verdejo A, Caminos E. TRPC1 Channels Are Expressed in Pyramidal Neurons and in a Subset of Somatostatin Interneurons in the Rat Neocortex. Front Neuroanat. 2018;12:15 pubmed publisher
	Liang M, Zhong W, Miao F, Wu H, Liu Y. Effects of losartan on vasomotor function and canonical transient receptor potential channels in the aortas of sinoaortic denervation rats. Clin Exp Hypertens. 2017;:1-10 pubmed publisher
	Scheede Bergdahl C, Bergdahl A. Adaptation of mitochondrial expression and ATP production in dedifferentiating vascular smooth muscle cells. Can J Physiol Pharmacol. 2017;95:1473-1479 pubmed publisher
	Qu Y, Wang L, Zhong H, Liu Y, Tang N, Zhu L, et al. TRPC1 stimulates calcium‑sensing receptor‑induced store‑operated Ca2+ entry and nitric oxide production in endothelial cells. Mol Med Rep. 2017;16:4613-4619 pubmed publisher
	Wajdner H, Farrington J, Barnard C, Peachell P, Schnackenberg C, Marino J, et al. Orai and TRPC channel characterization in FcεRI-mediated calcium signaling and mediator secretion in human mast cells. Physiol Rep. 2017;5: pubmed publisher
	Sun Y, Zhang H, Selvaraj S, Sukumaran P, Lei S, Birnbaumer L, et al. Inhibition of L-Type Ca2+ Channels by TRPC1-STIM1 Complex Is Essential for the Protection of Dopaminergic Neurons. J Neurosci. 2017;37:3364-3377 pubmed publisher
	Du T, Rong Y, Feng R, Verkhratsky A, Peng L. Chronic Treatment with Anti-bipolar Drugs Down-Regulates Gene Expression of TRPC1 in Neurones. Front Cell Neurosci. 2016;10:305 pubmed publisher
	Jiang H, Zeng B, Chen G, Lai B, Lu S, Qu J. Lipopolysaccharide potentiates endothelin-1-induced proliferation of pulmonary arterial smooth muscle cells by upregulating TRPC channels. Biomed Pharmacother. 2016;82:20-7 pubmed publisher
	Wang W, Gu L, Verkhratsky A, Peng L. Ammonium Increases TRPC1 Expression Via Cav-1/PTEN/AKT/GSK3? Pathway. Neurochem Res. 2017;42:762-776 pubmed publisher
	Jiang Q, Lu W, Yang K, Hadadi C, Fu X, Chen Y, et al. Sodium tanshinone IIA sulfonate inhibits hypoxia-induced enhancement of SOCE in pulmonary arterial smooth muscle cells via the PKG-PPAR-? signaling axis. Am J Physiol Cell Physiol. 2016;311:C136-49 pubmed publisher
	Faouzi M, Hague F, Geerts D, Ay A, Potier Cartereau M, Ahidouch A, et al. Functional cooperation between KCa3.1 and TRPC1 channels in human breast cancer: Role in cell proliferation and patient prognosis. Oncotarget. 2016;7:36419-36435 pubmed publisher
	Lin A, Sun H, Paudel O, Lin M, Sham J. Conformation of ryanodine receptor-2 gates store-operated calcium entry in rat pulmonary arterial myocytes. Cardiovasc Res. 2016;111:94-104 pubmed publisher
	Du L, Wang L, Yang X, Wang P, Li X, Chai D, et al. Transient Receptor Potential-canonical 1 is Essential for Environmental Enrichment-Induced Cognitive Enhancement and Neurogenesis. Mol Neurobiol. 2017;54:1992-2002 pubmed publisher
	Badr H, Kozai D, Sakaguchi R, Numata T, Mori Y. Different Contribution of Redox-Sensitive Transient Receptor Potential Channels to Acetaminophen-Induced Death of Human Hepatoma Cell Line. Front Pharmacol. 2016;7:19 pubmed publisher
	Ryazantseva M, Skobeleva K, Glushankova L, Kaznacheyeva E. Attenuated presenilin-1 endoproteolysis enhances store-operated calcium currents in neuronal cells. J Neurochem. 2016;136:1085-95 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-TRPC1 Antibody (#ACC-010), (1:200).2. Anti-TRPC1 Antibody, preincubated with TRPC1 Blocking Peptide (#BLP-CC010).

image 2 :

Expression of TRPC1 in mouse cerebellum - Immunohistochemical staining of mouse cerebellum frozen sections using Anti-TRPC1 Antibody (#ACC-010). A. TRPC1 (red) appears in Purkinje cells (arrows) and in the molecular (Mol) and granule (Gran) layers. B. Staining with mouse anti-parvalbumin (PV) in the same brain section. C. Confocal merge of TRPC1 and PV demonstrates partial co-localization in the Purkinje and the molecular layers.

image 3 :

Western blot analysis of human Jurkat T-cell leukemia cell line lysate: - 1. Anti-Semaphorin 7A (SEMA7A) (extracellular) Antibody(#ASR-077) (1:400).2. Anti-Semaphorin 7A (SEMA7A) (extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

ACC-010

SKU :

ACC-010-CF_0.2 ml

Product Name :

Anti-TRPC1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P48995

Applications :

IC IF IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC010

Homology :

Mouse, rat, human - identical; rabbit, bovine - 14/15 amino acid residues identical; Xenopus Laevis - 11/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

QLYDKGYTSKEQKDC, corresponding to amino acid residues 557-571 of human TRPC1

Product Page - Scientific background :

The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel families and consists of diverse groups of proteins. In mammals, about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels.1-4The TRPC subfamily consists of seven proteins named TRPC1 to 7, which can be further divided into four subgroups based on their sequence homology and functional similarities: 1) TRPC1 2) TRPC4 and TRPC5 3) TRPC3, TRPC6, TRPC7 4) TRPC2.2,5 They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues.TRPC1 was the first mammalian TRP protein that was reported to form an ion channel.2 It can co-assemble with other TRPC subunits (TRPC3, TRPC4, TRPC5) to form heterotetramers whose properties are distinct from that of their homomeric form. The existence of the TRPC1 homomers has not been established as yet.1-3The TRPC1, TRPC4 and TRPC5 can be activated either by Ca2+ store depletion or by GPCR stimulation pathways, while TRPC3, TRPC6 and TRPC7 form non-selective cationic channels that are activated by the stimulation of GPCRs. TRPC1, 4 and TRPC5 are assumed to form components of store operated channels in some cell types such as salivary gland cells, endothelial cells and vascular smooth muscle cells.6

Applications may also work in :

IC IF IHC IP WB

Supplier :

Alomone Labs

Target :

Short transient receptor potential channel 1, Transient receptor protein 1, TRP1

Short Description :

A Rabbit Polyclonal Antibody to TRPC1 Channel

Long Description :

Anti-TRPC1 Antibody (#ACC-010) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize TRPC1 from human, mouse, and rat samples.

Negative Control :

BLP-CC010

Positive Control :

Synonyms :

Short transient receptor potential channel 1, Transient receptor protein 1, TRP1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRPC1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP IHC ICC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage