Anti-CaV3.3 (CACNA1I) Antibody | Alomone Labs ACC-009 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CaV3.3 (CACNA1I) Antibody

catalog :

ACC-009

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section

more info or order :

Alomone Labs product webpage

citations: 15

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 1m	El Khoueiry C, Cabungcal J, Rov xf3 Z, Fournier M, Do K, Steullet P. Developmental oxidative stress leads to T-type Ca2+ channel hypofunction in thalamic reticular nucleus of mouse models pertinent to schizophrenia. Mol Psychiatry. 2022;27:2042-2051 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 7i	Zhang Y, Qian Z, Jiang D, Sun Y, Gao S, Jiang X, et al. Neuromedin B receptor stimulation of Cav3.2 T-type Ca2+ channels in primary sensory neurons mediates peripheral pain hypersensitivity. Theranostics. 2021;11:9342-9357 pubmed publisher
	Contreras E, Bhoi J, Sonoda T, Birnbaumer L, Schmidt T. Melanopsin activates divergent phototransduction pathways in intrinsically photosensitive retinal ganglion cell subtypes. elife. 2023;12: pubmed publisher
	Eickhoff A, Tjaden J, Stahlke S, Vorgerd M, Theis V, Matschke V, et al. Effects of progesterone on T-type-Ca2+-channel expression in Purkinje cells. Neural Regen Res. 2022;17:2465-2471 pubmed publisher
	Steinecke A, Bolton M, Taniguchi H. Neuromodulatory control of inhibitory network arborization in the developing postnatal neocortex. Sci Adv. 2022;8:eabe7192 pubmed publisher
	Tjaden J, Eickhoff A, Stahlke S, Gehmeyr J, Vorgerd M, Theis V, et al. Expression Pattern of T-Type Ca2+ Channels in Cerebellar Purkinje Cells after VEGF Treatment. Cells. 2021;10: pubmed publisher
	Mayadali Ü, Fleuriet J, MUSTARI M, Straka H, Horn A. Transmitter and ion channel profiles of neurons in the primate abducens and trochlear nuclei. Brain Struct Funct. 2021;226:2125-2151 pubmed publisher
	Montera M, Goins A, Cmarko L, Weiss N, Westlund K, Alles S. Trigeminal neuropathic pain is alleviated by inhibition of Cav3.3 T-type calcium channels in mice. Channels (Austin). 2021;15:31-37 pubmed publisher
	Sun H, Zhang H, Ross A, Wang T, Al Chami A, Wu S. Developmentally Regulated Rebound Depolarization Enhances Spike Timing Precision in Auditory Midbrain Neurons. Front Cell Neurosci. 2020;14:236 pubmed publisher
	Mart xed nez Hern xe1 ndez E, Zeglin A, Almazan E, Perissinotti P, He Y, KOOB M, et al. KLHL1 Controls CaV3.2 Expression in DRG Neurons and Mechanical Sensitivity to Pain. Front Mol Neurosci. 2019;12:315 pubmed publisher
	Yang T, He M, Zhang H, Barrett P, Hu C. L- and T-type calcium channels control aldosterone production from human adrenals. J Endocrinol. 2020;244:237-247 pubmed publisher
	Cheng X, Ma L, Feng X, Zhu M, Zhang D, Xu L, et al. Antigen retrieval pre-treatment causes a different expression pattern of Cav3.2 in rat and mouse spinal dorsal horn. Eur J Histochem. 2019;63: pubmed publisher
	Dong L, Cheng X, Zhou L, Hu Y. Calcium channels are involved in EphB/ephrinB reverse signaling?induced apoptosis in a rat chronic ocular hypertension model. Mol Med Rep. 2017;: pubmed publisher
	Fan J, Gandini M, Zhang F, Chen L, Souza I, Zamponi G. Down-regulation of T-type Cav3.2 channels by hyperpolarization-activated cyclic nucleotide-gated channel 1 (HCN1): Evidence of a signaling complex. Channels (Austin). 2017;11:434-443 pubmed publisher
	Petersen A, Jensen C, Crepel V, Falkerslev M, Perrier J. Serotonin Regulates the Firing of Principal Cells of the Subiculum by Inhibiting a T-type Ca2+ Current. Front Cell Neurosci. 2017;11:60 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-CaV3.3 (CACNA1I) Antibody (#ACC-009), (1:200). 2. Anti-CaV3.3 (CACNA1I) Antibody, preincubated with Cav3.3/CACNA1I Blocking Peptide (#BLP-CC009).

image 2 :

Expression of CaV3.3 in mouse hippocampus. - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-CaV3.3 (CACNA1I) Antibody (#ACC-009), (1:400), followed by donkey anti-rabbit-Cy3. A. Staining in the hippocampal CA3 region, showed Cav3.3 immunoreactivity (red) appeared in soma of pyramidal neuron profiles (horizontal arrows) and in dendrites (vertical arrows) projecting into the stratum radiatum (SR). B. Pre-incubation of the antibody with Cav3.3/CACNA1I Blocking Peptide (#BLP-CC009), suppressed staining. Cell nuclei are stained with DAPI (blue). P = pyramidal layer.

image 3 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4)brain membranes: - 12. Anti-Semaphorin 7A (SEMA7A) (extracellular) Antibody(#ASR-077) (1:200).34. Anti-Semaphorin 7A (SEMA7A) (extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

ACC-009

SKU :

ACC-009-CF_0.2 ml

Product Name :

Anti-CaV3.3 (CACNA1I) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9Z0Y8

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC009

Homology :

Human - 14/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, between domains II and III

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CNGRMPNIAKDVFTK, corresponding to amino acid residues 1053-1067 of rat CaV3.3

Product Page - Scientific background :

T-type Ca2+ channels play an important role in many cellular processes such as hormone secretion, neurotransmitter release and cell differentiation. T-type channels are also known to participate in the pacemaker activities of the heart and neurons including thalamic neurons.1 Three genes encoding T-type Ca2+ channels have been cloned and designated as CaV3.1 (CACNA1G), CaV3.2 (CACNA1H) and CaV3.3 (CACNA1I).1-3 While CaV3.1 and CaV3.2 are widely expressed in various tissues, CaV3.3 is primarily expressed in the central nervous system, where high expression has been described in thalamic neurons. The Ca2+ current generated by the CaV3.3 channel displays much slower activation and inactivation compared to the currents produced by CaV3.1 and CaV3.2, suggesting it might play a different role in neuronal excitability.1,4

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Voltage-dependent T-type calcium channel subunit α1I

Short Description :

A Rabbit Polyclonal Antibody to CaV3.3 (CACNA1I) Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the CaV3.3 channel. Anti-CaV3.3 (CACNA1I) Antibody (#ACC-009) can be used in western blot, immunohistochemical and immunocytochemical applications. It has been designed to recognize CaV3.3 from rat, human and mouse samples.

Negative Control :

BLP-CC009

Positive Control :

Synonyms :

Voltage-dependent T-type calcium channel subunit α1I

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CACNA1I

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC ICC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage