Anti-CACNB3 Antibody | Alomone Labs ACC-008 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CACNB3 Antibody

catalog :

ACC-008

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

domestic rabbit

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Al Katat A, Zhao J, Calderone A, Parent L. Sympathetic Stimulation Upregulates the Ca2+ Channel Subunit, CaVα2δ1, via the β1 and ERK 1/2 Pathway in Neonatal Ventricular Cardiomyocytes. Cells. 2022;11: pubmed publisher
Segura E, Mehta A, Marsolais M, Quan X, Zhao J, Sauv xe9 R, et al. An ancestral MAGUK protein supports the modulation of mammalian voltage-gated Ca2+ channels through a conserved CaVβ-like interface. Biochim Biophys Acta Biomembr. 2020;1862:183439 pubmed publisher
Weinman M, Fischer J, Jacobs D, Goodall C, Bracha S, Chappell P. Autocrine production of reproductive axis neuropeptides affects proliferation of canine osteosarcoma in vitro. BMC Cancer. 2019;19:158 pubmed publisher
Kuang H, Tang Z, Zhang C, Wang Z, Li W, Yang C, et al. Taxifolin Activates the Nrf2 Anti-Oxidative Stress Pathway in Mouse Skin Epidermal JB6 P+ Cells through Epigenetic Modifications. Int J Mol Sci. 2017;18: pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-CACNB3 Antibody (#ACC-008), (1:200).2. Anti-CACNB3 Antibody, preincubated with CACNB3 Blocking Peptide (#BLP-CC008).

image 2 :

Expression of CACNB3 (CaVβ3) in rat hippocampus - Immunohistochemical staining of rat hippocampus with Anti-CACNB3 Antibody (#ACC-008). A. CaVβ3 (red) appears in neurons (arrows). B. Staining of nerve cells with mouse anti-parvalbumin demonstrates the restriction of CaVβ3 to cell bodies. C. Merged image of panels A and B.

image 3 :

Peptide (C)RKQEYNGKIPRFRR corresponding to amino acid residues 190-203 of mouse SEMA7A (Accession Q9QUR8). Extracellular.

product information

CAT :

ACC-008

SKU :

ACC-008-CF_0.2 ml

Product Name :

Anti-CACNB3 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P54286

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC008

Homology :

Rat, mouse, human, Xenopus - identical; bovine - 14/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rabbit

Sequence :

(C)DRNWQRNRPWPKDSY, corresponding to amino acid residues 463-477 of rabbit CACNB3

Product Page - Scientific background :

The β3 protein is an auxiliary subunit of the CaV (voltage-dependent calcium) channel multimer complex. Four β isoforms were identified in mammalian genomes and they share some sequence as well as structure homology.1-3 The structure of CaVβ3 was recently determined (as well as other CaVβ subunits; for review see reference 3). CaVβ subunits are cytoplasmic proteins, that bind the cytoplasmic face of the pore forming CaV α1 subunits. These interactions have profound effects on channel trafficking to the plasma membrane as well as on current parameters such as voltage-dependent kinetics and interactions with modulatory proteins.1,2 CaVβ3 might interact with all the pore-forming subunits that have been tested and in vivo is expressed in smooth muscle, trachea, aorta, lung and brain.1

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

CaVβ3, CAB3, CACNLB3, Voltage-dependent L-type calcium channel subunit beta-3

Short Description :

A Rabbit Polyclonal Antibody to CACNB3 (CaV?3) Subunit

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rabbit CaVβ3. Anti-CACNB3 Antibody (#ACC-008) can be used in western blot, immunohistochemistry and immunocytochemistry applications. The antibody has been designed to recognize CaVβ3 from rat, mouse and human samples.

Negative Control :

BLP-CC008

Positive Control :

Synonyms :

CaVβ3, CAB3, CACNLB3, Voltage-dependent L-type calcium channel subunit beta-3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CACNB3

AB Specifictiy :

Identical for all splice forms.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage