Anti-CaV2.3 (CACNA1E) Antibody | Alomone Labs ACC-006 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CaV2.3 (CACNA1E) Antibody

catalog :

ACC-006

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 7

Reference
Marseglia M, Amaro A, Solari N, Gangemi R, Croce E, Tanda E, et al. How to Make Immunotherapy an Effective Therapeutic Choice for Uveal Melanoma. Cancers (Basel). 2021;13: pubmed publisher
Bhandari P, Vandael D, Fernández Fernández D, Fritzius T, Kleindienst D, Önal C, et al. GABAB receptor auxiliary subunits modulate Cav2.3-mediated release from medial habenula terminals. elife. 2021;10: pubmed publisher
Segura E, Mehta A, Marsolais M, Quan X, Zhao J, Sauv xe9 R, et al. An ancestral MAGUK protein supports the modulation of mammalian voltage-gated Ca2+ channels through a conserved CaVβ-like interface. Biochim Biophys Acta Biomembr. 2020;1862:183439 pubmed publisher
Li Q, Zhu Z, Lu J, Chao Y, Zhou X, Huang Y, et al. Sleep deprivation of rats increases postsurgical expression and activity of L-type calcium channel in the dorsal root ganglion and slows recovery from postsurgical pain. Acta Neuropathol Commun. 2019;7:217 pubmed publisher
Smith M, Katsouri L, Virtue S, Choudhury A, Vidal Puig A, Ashford M, et al. Calcium Channel CaV2.3 Subunits Regulate Hepatic Glucose Production by Modulating Leptin-Induced Excitation of Arcuate Pro-opiomelanocortin Neurons. Cell Rep. 2018;25:278-287.e4 pubmed publisher
Gandla J, Lomada S, Lu J, Kuner R, Bali K. miR-34c-5p functions as pronociceptive microRNA in cancer pain by targeting Cav2.3 containing calcium channels. Pain. 2017;158:1765-1779 pubmed publisher
Gorin M, Tsitoura C, Kahan A, Watznauer K, Drose D, Arts M, et al. Interdependent Conductances Drive Infraslow Intrinsic Rhythmogenesis in a Subset of Accessory Olfactory Bulb Projection Neurons. J Neurosci. 2016;36:3127-44 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-CaV2.3 (CACNA1E) Antibody (#ACC-006), (1:200).2. Anti-CaV2.3 (CACNA1E) Antibody, preincubated with Cav2.3/CACNA1E Blocking Peptide (#BLP-CC006).

image 2 :

Expression of CaV2.3 in mouse cerebellum - Immunohistochemical staining of mouse cerebellum with Anti-CaV2.3 (CACNA1E) Antibody (#ACC-006). CaV2.3 (red) appears in both soma and dendritic trees of several Purkinje cells. Staining of neurofilament 200 (green) in the same brain section demonstrates the relationship between axons passing through the granule layer and the Purkinje cells.

image 3 :

Expression of SERCA3 in rat cerebellum - Immunohistochemical staining of perfusion-fixed frozen rat brain sections withAnti-SERCA3 Antibody(#ACP-014) (1:200) followed by goat anti-rabbit-AlexaFluor-488. SERCA3 immunoreactivity (green) appears in the molecular layer (MOL) and Purkinje cells (arrows) but not in the granule layer (GL) or cerebellum sections. Cell nuclei are stained with DAPI (blue).

product information

CAT :

ACC-006

SKU :

ACC-006-CF_0.2 ml

Product Name :

Anti-CaV2.3 (CACNA1E) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q07652

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC006

Homology :

Mouse - 14/16 amino acid residues identical; rabbit - 12/16 amino acid residues identical; human - 11/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular loop between domains II and III

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)SASQERSLDEGVSIDG, corresponding to amino acid residues 892-907 of rat CaV2.3

Product Page - Scientific background :

Voltage-dependent Ca2+ channels (CaV channels) are pivotal players in many physiological roles such as secretion, contraction, migration and excitation.1The voltage-dependent Ca2+ channels are composed of several subunits; α1, β, α2δ and γ. CaV channels were originally divided into six physiological types: L-, N-, P-, Q-, R-, and T-type.1The CaV2.3 (formerly named α1E) protein, forms the R-type channels. R-type channels are highly sensitive to both SNX-482 (#RTS-500) and NiCl2. CaV2.3 channels were shown to mediate fast excitatory transmission in several synapses including the hippocampus.2CaV2.3 channels can be modulated by the muscarinic G-protein coupled receptors. In HEK-293 cells, expressing CaV2.3 channels, the R-type channel was shown to be both facilitated and inhibited by m1 and m2 muscarinic receptors. However, inhibition was much weaker than facilitation for the m1 receptor, while for m2 receptors, inhibition was much more pronounced than activation.3Facilitation and inhibition by the same receptors was probably exerted via different pathways as was indicated by their response to various inhibitors.3

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

CACH6, CACNL1A6, Voltage-dependent R-type calcium channel subunit α1E, BII, Brain calcium channel II, RBE2

Short Description :

A Rabbit Polyclonal Antibody to CaV2.3 (CACNA1E) Channel

Long Description :

Alomone Labs is pleased to offer an antibody raised against an epitope of the rat CaV2.3 channel. Anti-CaV2.3 (CACNA1E) Antibody (#ACC-006) can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize CaV2.3 from human, mouse and rat samples.

Negative Control :

BLP-CC006

Positive Control :

Synonyms :

CACH6, CACNL1A6, Voltage-dependent R-type calcium channel subunit α1E, BII, Brain calcium channel II, RBE2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CACNA1E

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP Electrophysiology

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage