Anti-CaV1.3 (CACNA1D) Antibody | Alomone Labs ACC-005 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CaV1.3 (CACNA1D) Antibody

catalog :

ACC-005

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 36

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; rat; loading ...; fig 1g	Vierra N, Kirmiz M, van der List D, Santana L, Trimmer J. Kv2.1 mediates spatial and functional coupling of L-type calcium channels and ryanodine receptors in mammalian neurons. elife. 2019;8: pubmed publisher
immunohistochemistry; mouse; 1:75; loading ...; fig 3c	Becker L, Schnee M, Niwa M, Sun W, Maxeiner S, Talaei S, et al. The presynaptic ribbon maintains vesicle populations at the hair cell afferent fiber synapse. elife. 2018;7: pubmed publisher
immunohistochemistry; mouse; 1:100; loading ...; fig 4a	Jean P, Lopez de la Morena D, Michanski S, Jaime Tobón L, Chakrabarti R, Picher M, et al. The synaptic ribbon is critical for sound encoding at high rates and with temporal precision. elife. 2018;7: pubmed publisher
	Casas M, Murray K, Hino K, Vierra N, Sim xf3 S, Trimmer J, et al. NPC1-dependent alterations in KV2.1-CaV1.2 nanodomains drive neuronal death in models of Niemann-Pick Type C disease. Nat Commun. 2023;14:4553 pubmed publisher
	Louradour J, Bortolotti O, Torre E, Bidaud I, Lamb N, Fernandez A, et al. L-Type Cav1.3 Calcium Channels Are Required for Beta-Adrenergic Triggered Automaticity in Dormant Mouse Sinoatrial Pacemaker Cells. Cells. 2022;11: pubmed publisher
	Boero L, Payne S, G xf3 mez Casati M, Rutherford M, Goutman J. Noise Exposure Potentiates Exocytosis From Cochlear Inner Hair Cells. Front Synaptic Neurosci. 2021;13:740368 pubmed publisher
	Hisatsune C, Shimada T, Miyamoto A, Lee A, Yamagata K. Tuberous Sclerosis Complex (TSC) Inactivation Increases Neuronal Network Activity by Enhancing Ca2+ Influx via L-Type Ca2+ Channels. J Neurosci. 2021;41:8134-8149 pubmed publisher
	Liu X, Zhao R, Ding Q, Yao X, Tsang S. TRPC7 regulates the electrophysiological functions of embryonic stem cell-derived cardiomyocytes. Stem Cell Res Ther. 2021;12:262 pubmed publisher
	Wang L, Lei Q, Zhao S, Xu W, Dong W, Ran J, et al. Ginkgolide B Maintains Calcium Homeostasis in Hypoxic Hippocampal Neurons by Inhibiting Calcium Influx and Intracellular Calcium Release. Front Cell Neurosci. 2020;14:627846 pubmed publisher
	Dickerson M, Dadi P, Butterworth R, Nakhe A, Graff S, Zaborska K, et al. Tetraspanin-7 regulation of L-type voltage-dependent calcium channels controls pancreatic β-cell insulin secretion. J Physiol. 2020;598:4887-4905 pubmed publisher
	Li B, He X, Dong Z, Xuan K, Sun W, Gao L, et al. Ionomycin ameliorates hypophosphatasia via rescuing alkaline phosphatase deficiency-mediated L-type Ca2+ channel internalization in mesenchymal stem cells. Bone Res. 2020;8:19 pubmed publisher
	Hofer N, Tuluc P, Ortner N, Nikonishyna Y, Fern xe1 ndes Quintero M, Liedl K, et al. Biophysical classification of a CACNA1D de novo mutation as a high-risk mutation for a severe neurodevelopmental disorder. Mol Autism. 2020;11:4 pubmed publisher
	Ortner N, Pinggera A, Hofer N, Siller A, Brandt N, Raffeiner A, et al. RBP2 stabilizes slow Cav1.3 Ca2+ channel inactivation properties of cochlear inner hair cells. Pflugers Arch. 2020;472:3-25 pubmed publisher
	Sahu G, Wazen R, Colarusso P, Chen S, Zamponi G, Turner R. Junctophilin Proteins Tether a Cav1-RyR2-KCa3.1 Tripartite Complex to Regulate Neuronal Excitability. Cell Rep. 2019;28:2427-2442.e6 pubmed publisher
	Qi F, Zhang R, Chen J, Zhao F, Sun Y, Du Z, et al. Down-regulation of Cav1.3 in auditory pathway promotes age-related hearing loss by enhancing calcium-mediated oxidative stress in male mice. Aging (Albany NY). 2019;11:6490-6502 pubmed publisher
	Eckrich S, Hecker D, Sorg K, Blum K, Fischer K, M xfc nkner S, et al. Cochlea-Specific Deletion of Cav1.3 Calcium Channels Arrests Inner Hair Cell Differentiation and Unravels Pitfalls of Conditional Mouse Models. Front Cell Neurosci. 2019;13:225 pubmed publisher
	Vincent P, Cho S, Tertrais M, Bouleau Y, von Gersdorff H, Dulon D. Clustered Ca2+ Channels Are Blocked by Synaptic Vesicle Proton Release at Mammalian Auditory Ribbon Synapses. Cell Rep. 2018;25:3451-3464.e3 pubmed publisher
	Brandenburg S, Pawlowitz J, Fakuade F, Kownatzki Danger D, Kohl T, Mitronova G, et al. Axial Tubule Junctions Activate Atrial Ca2+ Release Across Species. Front Physiol. 2018;9:1227 pubmed publisher
	Kamijo S, Ishii Y, Horigane S, Suzuki K, Ohkura M, Nakai J, et al. A Critical Neurodevelopmental Role for L-Type Voltage-Gated Calcium Channels in Neurite Extension and Radial Migration. J Neurosci. 2018;38:5551-5566 pubmed publisher
	Neef J, Urban N, Ohn T, Frank T, Jean P, Hell S, et al. Quantitative optical nanophysiology of Ca2+ signaling at inner hair cell active zones. Nat Commun. 2018;9:290 pubmed publisher
	Krinner S, Butola T, Jung S, Wichmann C, Moser T. RIM-Binding Protein 2 Promotes a Large Number of CaV1.3 Ca2+-Channels and Contributes to Fast Synaptic Vesicle Replenishment at Hair Cell Active Zones. Front Cell Neurosci. 2017;11:334 pubmed publisher
	Michalski N, Goutman J, Auclair S, Boutet de Monvel J, Tertrais M, Emptoz A, et al. Otoferlin acts as a Ca2+ sensor for vesicle fusion and vesicle pool replenishment at auditory hair cell ribbon synapses. elife. 2017;6: pubmed publisher
	Sahu G, Asmara H, Zhang F, Zamponi G, Turner R. Activity-Dependent Facilitation of CaV1.3 Calcium Channels Promotes KCa3.1 Activation in Hippocampal Neurons. J Neurosci. 2017;37:11255-11270 pubmed publisher
	Luo F, Liu X, Sudhof T, Acuna C. Efficient stimulus-secretion coupling at ribbon synapses requires RIM-binding protein tethering of L-type Ca2+ channels. Proc Natl Acad Sci U S A. 2017;114:E8081-E8090 pubmed publisher
	Shi L, Ko M, Ko G. Retinoschisin Facilitates the Function of L-Type Voltage-Gated Calcium Channels. Front Cell Neurosci. 2017;11:232 pubmed publisher
	Kim S, Park Y, Lee B, Choi B, Kim H, Kim C. Reduction of Cav1.3 channels in dorsal hippocampus impairs the development of dentate gyrus newborn neurons and hippocampal-dependent memory tasks. PLoS ONE. 2017;12:e0181138 pubmed publisher
	Fan F, Matsunaga K, Wang H, Ishizaki R, Kobayashi E, Kiyonari H, et al. Exophilin-8 assembles secretory granules for exocytosis in the actin cortex via interaction with RIM-BP2 and myosin-VIIa. elife. 2017;6: pubmed publisher
	Busch C, Annamalai B, Abdusalamova K, Reichhart N, Huber C, Lin Y, et al. Anaphylatoxins Activate Ca2+, Akt/PI3-Kinase, and FOXO1/FoxP3 in the Retinal Pigment Epithelium. Front Immunol. 2017;8:703 pubmed publisher
	Picher M, Oprişoreanu A, Jung S, Michel K, Schoch S, Moser T. Rab Interacting Molecules 2 and 3 Directly Interact with the Pore-Forming CaV1.3 Ca2+ Channel Subunit and Promote Its Membrane Expression. Front Cell Neurosci. 2017;11:160 pubmed publisher
	Axelsson A, Mahdi T, Nenonen H, Singh T, Hänzelmann S, Wendt A, et al. Sox5 regulates beta-cell phenotype and is reduced in type 2 diabetes. Nat Commun. 2017;8:15652 pubmed publisher
	Sun Y, Zhang H, Selvaraj S, Sukumaran P, Lei S, Birnbaumer L, et al. Inhibition of L-Type Ca2+ Channels by TRPC1-STIM1 Complex Is Essential for the Protection of Dopaminergic Neurons. J Neurosci. 2017;37:3364-3377 pubmed publisher
	Cao M, Wu Y, Ashrafi G, McCartney A, Wheeler H, Bushong E, et al. Parkinson Sac Domain Mutation in Synaptojanin 1 Impairs Clathrin Uncoating at Synapses and Triggers Dystrophic Changes in Dopaminergic Axons. Neuron. 2017;93:882-896.e5 pubmed publisher
	Vincent P, Bouleau Y, Charpentier G, Emptoz A, Safieddine S, Petit C, et al. Different CaV1.3 Channel Isoforms Control Distinct Components of the Synaptic Vesicle Cycle in Auditory Inner Hair Cells. J Neurosci. 2017;37:2960-2975 pubmed publisher
	Ohn T, Rutherford M, Jing Z, Jung S, Duque Afonso C, Hoch G, et al. Hair cells use active zones with different voltage dependence of Ca2+ influx to decompose sounds into complementary neural codes. Proc Natl Acad Sci U S A. 2016;113:E4716-25 pubmed publisher
	Philippart F, Destreel G, Merino Sepúlveda P, Henny P, Engel D, Seutin V. Differential Somatic Ca2+ Channel Profile in Midbrain Dopaminergic Neurons. J Neurosci. 2016;36:7234-45 pubmed publisher
	Walton R, Jones S, Rostron K, Kayani A, Close G, McArdle A, et al. Interactions of Short-Term and Chronic Treadmill Training With Aging of the Left Ventricle of the Heart. J Gerontol A Biol Sci Med Sci. 2016;71:1005-13 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-CaV1.3 (CACNA1D) Antibody (#ACC-005), (1:200).2. Anti-CaV1.3 (CACNA1D) Antibody, preincubated with Cav1.3/CACNA1D Blocking Peptide (#BLP-CC005).

image 2 :

Expression of CaV1.3 in rat DRG - Immunohistochemical staining of adult rat dorsal root ganglion (DRG) with Anti-CaV1.3 (CACNA1D) Antibody (#ACC-005). Staining appears in clusters of cells (green) but not in axons. Staining of neurofilament 200 demonstrates partial overlap with CaV1.3 in neuronal staining but not in axons (red).

product information

CAT :

ACC-005

SKU :

ACC-005-CF_0.2 ml

Product Name :

Anti-CaV1.3 (CACNA1D) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P27732

Applications :

IC IF IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC005

Homology :

Hamster, mouse - 16/17 amino acid residues identical; human -15/17 amino acid residues identical; chicken - 12/17 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular loop between domains II and III

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DNKVTIDDYQEEAEDKD, corresponding to amino acid residues 859-875 of rat CaV1.3

Product Page - Scientific background :

All L-type calcium channels are encoded by one of the CaV1 channel genes. These channels play a major role as a Ca2+ entry pathway in skeletal cardiac and smooth muscles as well as in neurons, endocrine cells and possibly in non-excitable cells such as hematopoetic and epithelial cells. All CaV1 channels are influenced by dihydropyridines (DHP) and are also referred to as DHP receptors. While the CaV1.1 and CaV1.4 isoforms are expressed in restricted tissues (skeletal muscle and retina, respectively), the expression of CaV1.2 is ubiquitous.1,2The CaV1.3 channels are also expressed, as are other L-type channels, in neurons and neuroendocrine cells. However, accumulated data has shown the expression of CaV1.3 in heart and suggests that it plays a major role in the generation of cardiac pacemaker activity.3,4Several peptidyl toxins have been described that are specific L-type channel blockers. These include the Mamba toxins Calcicludine (#SPC-650), Calciseptine (#C-500) and FS-2 (#F-700). So far no selective blocker for one of the CaV1 isoforms has been described.

Applications may also work in :

IC IF IHC IP WB

Supplier :

Alomone Labs

Target :

Voltage-dependent L-type calcium channel subunit α1D

Short Description :

A Rabbit Polyclonal Antibody to CaV1.3 (CACNA1D) Channel

Long Description :

Anti-CaV1.3 (CACNA1D) Antibody (#ACC-005) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize CaV1.3 from mouse, rat, and human samples.

Negative Control :

BLP-CC005

Positive Control :

Synonyms :

Voltage-dependent L-type calcium channel subunit α1D

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CACNA1D

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP IHC ICC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage