Guinea pig Anti-CaV1.3 (CACNA1D) Antibody | Alomone Labs ACC-005-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-CaV1.3 (CACNA1D) Antibody

catalog :

ACC-005-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Busch C, Annamalai B, Abdusalamova K, Reichhart N, Huber C, Lin Y, et al. Anaphylatoxins Activate Ca2+, Akt/PI3-Kinase, and FOXO1/FoxP3 in the Retinal Pigment Epithelium. Front Immunol. 2017;8:703 pubmed publisher

image

image 1 :

Western blot analysis of mouse brain (lanes 1 and 4), rat brain (lanes 2 and 5) and human brain neuroblastoma cells (SH-SY5Y) (lanes 3 and 6): - 1-3. Guinea pig Anti-CaV1.3 (CACNA1D) Antibody (#ACC-005-GP), (1:200).4-6. Guinea pig Anti-CaV1.3 (CACNA1D) Antibody, preincubated with Cav1.3/CACNA1D Blocking Peptide (#BLP-CC005). Following a broad screen of secondary antibodies, the following were used for this application:Western blot analysis:#106-035-006 (Jackson ImmunoResearch) #A7289 (Sigma)

image 2 :

Expression of CaV1.3 in rat DRGs - Immunohistochemical staining of rat dorsal root ganglion (DRG) using Guinea pig Anti-CaV1.3 (CACNA1D) Antibody (#ACC-005-GP), (1:200). A. CaV1.3 staining (red) is detected in some DRG cells (arrows). B. Nuclei staining using DAPI as the counterstain. C. Merged image of panels A and B.

product information

CAT :

ACC-005-GP

SKU :

ACC-005-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-CaV1.3 (CACNA1D) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P27732

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-CC005

Homology :

Mouse - 16/17 amino acid residues identical; human -15/17 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular loop between domains II and III

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DNKVTIDDYQEEAEDKD, corresponding to amino acid residues 859-875 of rat CaV1.3

Product Page - Scientific background :

The CaV1.3 channel belongs to the L-type family of Ca2+ channels. There are currently four known members in this family. L-type channels provide the main influx pathway for Ca2+, an essential, versatile and universal intracellular messenger.CaV1.3, as well as the other members of its family, is comprised of an α1-subunit that serves as the voltage sensitive pore. This subunit is comprised of transmembrane segments S1-S4 and a pore-forming region comprised of S5 and S6 segments together with their connecting linker, which contains helical regions contributing to the formation of the selectivity filter. The α1-subunit associates with other subunits to form hetero-oligomeric complexes. β-subunits are tightly associated at the cytoplasmic face of α1, whereas α2δ subunits are GPI-anchored to the plasma membrane and interact with the extracellular domains of α1. The long cytoplasmic C-terminal region of the α1 subunits serves as an important modulatory domain and is a target of numerous protein-protein interactions.CaV1.3 is expressed in many tissues such as the sinoatrial node, heart atria, neurons, chromaffin cells and pancreatic islets. Currently available calcium channel blocking therapeutic agents block CaV1.2 and CaV1.3 making it difficult to distinguish between the two. To this day there are no known human diseases resulting from mutations in the CACNA1D gene encoding the CaV1.3 α1 subunit. This could be due to the fact that loss-of-function mutations cause no phenotype in the heterozygous state (in mice) but are lethal in the homozygous state1,2.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Voltage-dependent L-type calcium channel subunit α1D

Short Description :

A Guinea Pig Polyclonal Antibody to CaV1.3 (CACNA1D) Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat CaV1.3 channel. Guinea pig Anti-CaV1.3 (CACNA1D) Antibody (#ACC-005-GP, formerly AGP-061), raised in guinea pigs, can be used in western blot analysis, and has been designed to recognize CaV1.3 from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-CaV1.3 (CACNA1D) Antibody (#ACC-005) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-CC005

Positive Control :

Synonyms :

Voltage-dependent L-type calcium channel subunit α1D

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CACNA1D

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage