Anti-CaVpan α1 Antibody | Alomone Labs ACC-004 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CaVpan α1 Antibody

catalog :

ACC-004

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-CaVpan α1 Antibody (#ACC-004), (1:200).2. Anti-CaVpan α1 Antibody, preincubated with Pan CaV α 1 Blocking Peptide (#BLP-CC004).

image 2 :

Western blot analysisof rat brain membranes: - 1.Anti-CaVpan ?1Antibody (#ACC-004) (1:200).2. Anti-CaVpan ?1 Antibody preincubated with the negative control antigen.

image 3 :

Peptide (C)DEALKYLSRHHVD corresponding to amino acid residues 981-993 of rat SERCA3 (Accession P18596). Cytoplasmic C-terminus.

product information

CAT :

ACC-004

SKU :

ACC-004-CF_0.2 ml

Product Name :

Anti-CaVpan α1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P22002

Applications :

IF IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC004

Homology :

CaV1 family (α1C, α1D, α1F, α1S) from all known species - identical; CaV2 family (α1A, α1B, α1E) from all known species - 17/19 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminal region.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DNFDYLTRDWSILGPHHLD, corresponding to amino acid residues 1506-1524 of rat CaV1.2

Product Page - Scientific background :

Ca2+ ions regulate many cellular processes such as secretion, proliferation, and apoptosis and they are also cofactors of many proteins.1,2 In neurons, Ca2+ entry through voltage-dependent Ca2+ (CaV) channels triggers neurotransmitter release. Fast synaptic transmission in the mammalian central nervous system (CNS) is mediated by several high voltage-dependent Ca2+ channels.3Five types of Ca2+ channels are expressed in the CNS: the L-type (CaV1), N-type (CaV2.2), P/Q-type (CaV2.1), R-type (CaV2.3), and the T-type (CaV3).4 Each CaV channel is a multimeric protein composed of a pore forming α1 subunit and the auxiliary β (CaVβ), α2δ and γ subunits. There are four known CaVβ subunits, in addition to four α2δ subunits and eight γ subunits.4

Applications may also work in :

IF IHC IP WB

Supplier :

Alomone Labs

Target :

Voltage-dependent L-type calcium channel subunit alpha-1C, Cacna1c

Short Description :

A Rabbit Pan Polyclonal Antibody to CaV1 and CaV2 Subunits

Long Description :

Alomone Labs is pleased to offer an antibody directed against an epitope of rat CaV1.2 channel that is identical in all isoforms of CaV1 and CaV2. Anti-CaVpan α1 Antibody (#ACC-004) can be used in western blot, immunoprecipitation and immunohistochemistry applications. It has been designed to recognize the high voltage-gated calcium channels, CaV1 and CaV2 subfamily members, from human, rat and mouse samples.

Negative Control :

BLP-CC004

Positive Control :

Synonyms :

Voltage-dependent L-type calcium channel subunit alpha-1C, Cacna1c

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CACNA1C

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage