Guinea pig Anti-CaV1.2 (CACNA1C) Antibody | Alomone Labs ACC-003-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-CaV1.2 (CACNA1C) Antibody

catalog :

ACC-003-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Szabó P, Ebner J, Koenig X, Hamza O, Watzinger S, Trojanek S, et al. Cardiovascular phenotype of the Dmdmdx rat - a suitable animal model for Duchenne muscular dystrophy. Dis Model Mech. 2021;14: pubmed publisher
Cheng P, Lin H, Chen Y, Cheng R, Su H, Huang R. The Na+/H+-Exchanger NHE1 Regulates Extra- and Intracellular pH and Nimodipine-sensitive [Ca2+]i in the Suprachiasmatic Nucleus. Sci Rep. 2019;9:6430 pubmed publisher
Lee M, Park C, Ha S, Park P, Berent R, Jorgensen B, et al. Serum response factor regulates smooth muscle contractility via myotonic dystrophy protein kinases and L-type calcium channels. PLoS ONE. 2017;12:e0171262 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membrane: - 1. Guinea pig Anti-CaV1.2 (CACNA1C) Antibody (#ACC-003-GP), (1:200).2. Guinea pig Anti-CaV1.2 (CACNA1C) Antibody, preincubated with Cav1.2/CACNA1C Blocking Peptide (#BLP-CC003).

image 2 :

Multiplex staining of CaV1.2 and GABA(A) α1 Receptor in rat cerebellum - Immunohistochemical staining of rat cerebellum using Guinea pig Anti-CaV1.2 (CACNA1C) Antibody (#ACC-003-GP) and Anti-GABA(A) α1 Receptor (extracellular)-ATTO Fluor-488 Antibody (#AGA-001-AG). A. CaV1.2 (red) is detected mostly in Purkinje cells (arrow). B. In the same section, GABA(A) α1 Receptor (green) is observed in the granule layer. C. Merge of the two images suggests some colocalization between CaV1.2 and GABA(A) α1 Receptor in the rat granule layer but only CaV1.2 appears in Purkinje cells.

product information

CAT :

ACC-003-GP

SKU :

ACC-003-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-CaV1.2 (CACNA1C) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P22002

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-CC003

Homology :

Mouse - identical; guinea pig - 17/18 amino acid residues identical; human, rabbit - 16/18 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular loop between domains II and III

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)TTKINMDDLQPSENEDKS, corresponding to amino acid residues 848-865 of rat CaV1.2

Product Page - Scientific background :

Voltage-gated Ca2+ channels (CaV), enable the passage of Ca2+ ions in a voltage dependent manner. These heteromeric entities are formed in part by the pore-forming α1 subunit which determines the biophysical and pharmacological properties of the channel1.L-type Ca2+ channels make up one of three voltage-gated Ca2+ channel families. Four different α1 isoforms (CaV1.1 to CaV1.4) belong to the L-type subfamily. Structurally, each α1 subunit has four homologous domains (I-IV) and each domain has a six transmembrane section. Like many other voltage-gated channels, L-type Ca2+ channels have auxiliary subunits which are responsible for modulating the surface expression and properties of the channels2-5.CaV1.1 is mostly expressed in the skeletal muscle, while CaV1.4 is mainly detected in the retina. The expression of both CaV1.2 and CaV1.3 is more extensive and includes neurons, heart, smooth muscle, inner ear, retina and pancreas6. L-type Ca2+ channels are involved in and modulate a variety of physiological functions such as muscle contraction, hormone secretion, neuronal excitability and gene expression5.CaV1.2 undergoes various post-translational modifications. For example, it can undergo proteolytic cleavage at its C-terminal. This cleavage has been shown to take place in neurons following the activation of NMDA receptors5,7 and in the heart5,8,9. The cleaved moiety can still interact with the channel and its general purpose is to modulate channel activity5. Other postranslation modifications of the channel include phosphorylation of CaV1.2 by a number of kinases such as PKA, PKC, Src and CaMKII5. In addition, it is not surprising that phosphatases also regulate channel activity, as they are required to antagonize the activity of the various kinases known to phosphorylate CaV1.2 5.The fact that CaV1.2 plays a prominent role in proper cardiac function has prompted endless studies regarding its regulation. Such studies have concluded that dysregulation of the channel leads to anomalies in heart contraction and thus heart failure5. Likewise, CaV1.2 defects have been detected in autism and bipolar disorder10.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Voltage-dependent L-type calcium channel subunit α1C

Short Description :

A Guinea Pig Polyclonal Antibody to CaV1.2 (CACNA1C) Channel

Long Description :

Guinea pig Anti-CaV1.2 (CACNA1C) Antibody (#ACC-003-GP, formerly AGP-001), raised in guinea pigs, is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize CaV1.2 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-CaV1.2 (CACNA1C) Antibody (#ACC-003) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-CC003

Positive Control :

Synonyms :

Voltage-dependent L-type calcium channel subunit α1C

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CACNA1C

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage