Anti-CaV1.2 (CACNA1C)-ATTO Fluor-488 Antibody | Alomone Labs ACC-003-AG product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CaV1.2 (CACNA1C)-ATTO Fluor-488 Antibody

catalog :

ACC-003-AG

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 488

clone name :

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Cserne Szappanos H, Muralidharan P, Ingley E, Petereit J, Millar A, Hool L. Identification of a novel cAMP dependent protein kinase A phosphorylation site on the human cardiac calcium channel. Sci Rep. 2017;7:15118 pubmed publisher

image

image 1 :

Expression of CaV1.2 in rat cerebellum - Immunohistochemical staining of rat cerebellum frozen sections with Anti-CaV1.2 (CACNA1C)-ATTO Fluor-488 Antibody (#ACC-003-AG), (1:100). Both dendrites of Purkinje cells (horizontal arrows) and fibers of Bergmann glia (vertical arrows) were stained. DAPI (blue Nissl counterstain) helps define the layers: granule (G), Purkinje (P) and molecular (M).

image 2 :

Expression of CaV1.2 in rat pancreas - Immunohistochemical staining of paraffin embedded sections of rat pancreas usingAnti-CaV1.2 (CACNA1C)-ATTO-488Antibody (#ACC-003-AG) (1:50). Staining is highly specific for endocrine cells of theIsle of Langerhans(IL).

image 3 :

Expression of CaV1.2 in rat cerebellum - Immunohistochemical staining of rat cerebellum frozen sections withAnti-CaV1.2 (CACNA1C)-ATTO-488Antibody (#ACC-003-AG) (1:100). Both dendrites of Purkinje cells (horizontal arrows) and fibers of Bergmann glia (vertical arrows) were stained. DAPI (blue Nissl counterstain) helps define the layers: granule (G) Purkinje (P) and molecular (M).

product information

CAT :

ACC-003-AG

SKU :

ACC-003-AG_50 mcl

Product Name :

Anti-CaV1.2 (CACNA1C)-ATTO Fluor-488 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P22002

Applications :

IC IF IHC

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC003

Homology :

Mouse - identical; guinea pig - 17/18 amino acid residues identical; human, rabbit - 16/18 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Intracellular loop between domains II and III

Label :

ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)TTKINMDDLQPSENEDKS, corresponding to amino acid residues 848-865 of rat CaV1.2

Product Page - Scientific background :

All L-type calcium channels are encoded by one of the CaV1 channel genes. These channels play a major role as a Ca2+ entry pathway in skeletal, cardiac and smooth muscles as well as in neurons, endocrine cells and possibly in non-excitable cells such as hematopoetic and epithelial cells. All CaV1 channels are influenced by dihydropyridines (DHP) and are also referred to as DHP receptors.While the CaV1.1 and CaV1.4 isoforms are expressed in restricted tissues (skeletal muscle and retina, respectively), the expression of CaV1.2 is ubiquitous and CaV1.3 channels are found in the heart, brain and pancreas. Several peptidyl toxins are described that are specific L-type channel blockers, but so far no selective blocker for one of the CaV1 isoforms have been described. These include the Mamba toxins Calcicludine (#SPC-650), Calciseptine (#C-500) and FS-2 (#F-700).

Applications may also work in :

IC IF IHC

Supplier :

Alomone Labs

Target :

Voltage-dependent L-type calcium channel subunit α1C

Short Description :

A Rabbit Polyclonal Antibody to CaV1.2 (CACNA1C) Channel Conjugated to the Fluorescent Dye ATTO-488

Long Description :

Anti-CaV1.2 (CACNA1C) Antibody (#ACC-003) is a highly specific antibody directed against an epitope of rat CaV1.2 channel. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, immunocytochemistry, and indirect flow cytometry applications. It has been designed to recognize CaV1.2 from mouse, rat, and human samples. Anti-CaV1.2 (CACNA1C)-ATTO Fluor-488 Antibody (#ACC-003-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-CaV1.2 (CACNA1C)-ATTO Fluor-488 Antibody has been tested in immunohistochemical and immunocytochemical applications and is specially suited to experiments requiring simultaneous labeling of different markers.

Negative Control :

RIC-001-AG

Positive Control :

Synonyms :

Voltage-dependent L-type calcium channel subunit α1C

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CACNA1C

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

ICC

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ACC-003), and immunohistochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:50-1:100

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage