Anti-CACNA1B (CaV2.2) Antibody | Alomone Labs ACC-002 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CACNA1B (CaV2.2) Antibody

catalog :

ACC-002

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 15

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; loading ...; fig 5c	Brocard J, Dufour F, Gory Fauré S, Arnoult C, Bosc C, Denarier E, et al. MAP6 interacts with Tctex1 and Cav 2.2/N-type calcium channels to regulate calcium signalling in neurons. Eur J Neurosci. 2017;: pubmed publisher
	Mart xed nez Hern xe1 ndez E, Zeglin A, Almazan E, Perissinotti P, He Y, KOOB M, et al. KLHL1 Controls CaV3.2 Expression in DRG Neurons and Mechanical Sensitivity to Pain. Front Mol Neurosci. 2019;12:315 pubmed publisher
	Li Q, Zhu Z, Lu J, Chao Y, Zhou X, Huang Y, et al. Sleep deprivation of rats increases postsurgical expression and activity of L-type calcium channel in the dorsal root ganglion and slows recovery from postsurgical pain. Acta Neuropathol Commun. 2019;7:217 pubmed publisher
	Gandini M, Souza I, Raval D, Xu J, Pan Y, Zamponi G. Differential regulation of Cav2.2 channel exon 37 variants by alternatively spliced μ-opioid receptors. Mol Brain. 2019;12:98 pubmed publisher
	Mishima K, Nakasatomi M, Takahashi S, Ikeuchi H, Sakairi T, Kaneko Y, et al. Attenuation of renal fibrosis after unilateral ureteral obstruction in mice lacking the N-type calcium channel. PLoS ONE. 2019;14:e0223496 pubmed publisher
	Gandini M, Souza I, Fan J, Li K, Wang D, Zamponi G. Interactions of Rabconnectin-3 with Cav2 calcium channels. Mol Brain. 2019;12:62 pubmed publisher
	Chen X, Liu D, Zhou D, Si Y, Xu D, Stamatkin C, et al. Small-molecule CaVα1⋅CaVβ antagonist suppresses neuronal voltage-gated calcium-channel trafficking. Proc Natl Acad Sci U S A. 2018;115:E10566-E10575 pubmed publisher
	Zhang D, Tu H, Cao L, Zheng H, Muelleman R, Wadman M, et al. Reduced N-Type Ca2+ Channels in Atrioventricular Ganglion Neurons Are Involved in Ventricular Arrhythmogenesis. J Am Heart Assoc. 2018;7: pubmed publisher
	Leo M, Schmitt L, Jastrow H, Thomale J, Kleinschnitz C, Hagenacker T. Cisplatin alters the function and expression of N-type voltage-gated calcium channels in the absence of morphological damage of sensory neurons. Mol Pain. 2017;13:1744806917746565 pubmed publisher
	Grimaldo L, Sandoval A, Garza López E, Felix R. Involvement of Parkin in the ubiquitin proteasome system-mediated degradation of N-type voltage-gated Ca2+ channels. PLoS ONE. 2017;12:e0185289 pubmed publisher
	Vivas O, Moreno C, Santana L, Hille B. Proximal clustering between BK and CaV1.3 channels promotes functional coupling and BK channel activation at low voltage. elife. 2017;6: pubmed publisher
	Larsen H, Bardsley E, Lefkimmiatis K, Paterson D. Dysregulation of Neuronal Ca2+ Channel Linked to Heightened Sympathetic Phenotype in Prohypertensive States. J Neurosci. 2016;36:8562-73 pubmed publisher
	Tang W, Thevathasan J, Lin Q, Lim K, Kuroda K, Kaibuchi K, et al. Stimulation of Synaptic Vesicle Exocytosis by the Mental Disease Gene DISC1 is Mediated by N-Type Voltage-Gated Calcium Channels. Front Synaptic Neurosci. 2016;8:15 pubmed publisher
	Ohno S, Yokoi H, Mori K, Kasahara M, Kuwahara K, Fujikura J, et al. Ablation of the N-type calcium channel ameliorates diabetic nephropathy with improved glycemic control and reduced blood pressure. Sci Rep. 2016;6:27192 pubmed publisher
	Forostyak O, Butenko O, Anderova M, Forostyak S, Sykova E, Verkhratsky A, et al. Specific profiles of ion channels and ionotropic receptors define adipose- and bone marrow derived stromal cells. Stem Cell Res. 2016;16:622-34 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-CACNA1B (CaV2.2) Antibody (#ACC-002), (1:200).2. Anti-CACNA1B (CaV2.2) Antibody, preincubated with the control antigen.

image 2 :

Expression of CaV2.2 in mouse cerebellum - Immunohistochemical staining of mouse cerebellum with Anti-CACNA1B (CaV2.2) Antibody (#ACC-002), (1:100). A. CaV2.2 (red) appears in Purkinje cells (arrows) and is distributed diffusely in the molecular layer (Mol). B. Staining of Purkinje cells with mouse anti-Calbindin 28K (green) demonstrates the restriction of CaV2.2 to cell bodies but not to dendrites in the molecular layer. C. Merged image of panels A and B.

image 3 :

Peptide (C)RHHRHRDRDKTSASTPA corresponding to amino acid residues 851-867 of rat CACNA1B(AccessionQ02294).Intracellular loop between domains II and III.

product information

CAT :

ACC-002

SKU :

ACC-002-CF_0.2 ml

Product Name :

Anti-CACNA1B (CaV2.2) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q02294

Applications :

IC IF IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC002

Homology :

Mouse - 15/17 amino acid residues identical; human 11/17 amino acid residues identical; rabbit - 11/17 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular loop between domains II and III

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RHHRHRDRDKTSASTPA, corresponding to amino acid residues 851-867 of rat CACNA1B

Product Page - Scientific background :

Voltage-dependent Ca2+ channels (CaV channels) are pivotal players in many physiological roles such as secretion, contraction, migration and excitation.1The voltage-dependent Ca2+ channels are composed of several subunits; α1, β, α2δ and γ. CaV channels were originally divided into six physiological types: L-, N-, P-, Q-, R-, and T-type.The CaV2.2 (formally named α1B) composes the α1 poreforming subunit for the N-type Ca2+ channel family. They are involved in neurotransmitter release from central neurons, including glutamate, γ-aminobutyric acid, acetylcholine, dopamine and noradrenaline.2The CaV2.2 is expressed preferentially in the central nervous system, where along with CaV2.1, it is responsible for pre-synaptic Ca2+ influx and neurotransmitter release.1,3The CaV2.2 channel is negatively regulated by many different GPCRs. There are two ways that this is done: either by directly binding Gβγ to the channel or by an indirect mechanism involving second messenger and channel phosphorylation.4 ω-Conotoxin GVIA (#C-300) is a specific blocker of CaV2.2 Ca2+ channels. It specifically blocks N-type CaV channels by binding to the CaV2.2 α1 subunit (α1B) and its action is only partially reversible.5,6

Applications may also work in :

IC IF IHC IP WB

Supplier :

Alomone Labs

Target :

Voltage-dependent N-type calcium channel subunit α1B, Brain calcium channel III, BIII

Short Description :

A Rabbit Polyclonal Antibody to CACNA1B (CaV2.2) Channel

Long Description :

Anti-CACNA1B (CaV2.2) Antibody (#ACC-002) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize CaV2.2 from mouse, rat, and human samples.

Negative Control :

BLP-CC002

Positive Control :

Synonyms :

Voltage-dependent N-type calcium channel subunit α1B, Brain calcium channel III, BIII

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CACNA1B

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage