Anti-CACNA1A (CaV2.1) Antibody | Alomone Labs ACC-001 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CACNA1A (CaV2.1) Antibody

catalog :

ACC-001

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 23

Reference
Coughlin G, Borsos M, Appling N, Barcelona B, Mayfield A, Mackey E, et al. Spatial genomics of AAVs reveals mechanism of transcriptional crosstalk that enables targeted delivery of large genetic cargo. bioRxiv. 2023;: pubmed publisher
Casas M, Murray K, Hino K, Vierra N, Sim xf3 S, Trimmer J, et al. NPC1-dependent alterations in KV2.1-CaV1.2 nanodomains drive neuronal death in models of Niemann-Pick Type C disease. Nat Commun. 2023;14:4553 pubmed publisher
Djillani A, Bazinet J, Catterall W. Synaptotagmin-7 Enhances Facilitation of Cav2.1 Calcium Channels. Eneuro. 2022;9: pubmed publisher
Mu xf1 oz Montero A, de Pascual R, S xe1 ez Mas A, Colmena I, Gand xed a L. Alterations of the Sympathoadrenal Axis Related to the Development of Alzheimer's Disease in the 3xTg Mouse Model. Biology (Basel). 2022;11: pubmed publisher
Gandini M, Souza I, Ferron L, Innes A, Zamponi G. The de novo CACNA1A pathogenic variant Y1384C associated with hemiplegic migraine, early onset cerebellar atrophy and developmental delay leads to a loss of Cav2.1 channel function. Mol Brain. 2021;14:27 pubmed publisher
Tepper B, Bartkowska K, Okrasa M, Ngati S, Braszak M, Turlejski K, et al. Downregulation of TrkC Receptors Increases Dendritic Arborization of Purkinje Cells in the Developing Cerebellum of the Opossum, Monodelphis domestica. Front Neuroanat. 2020;14:56 pubmed publisher
Shemesh O, Linghu C, Piatkevich K, Goodwin D, Celiker O, Gritton H, et al. Precision Calcium Imaging of Dense Neural Populations via a Cell-Body-Targeted Calcium Indicator. Neuron. 2020;107:470-486.e11 pubmed publisher
Mart xed nez Hern xe1 ndez E, Zeglin A, Almazan E, Perissinotti P, He Y, KOOB M, et al. KLHL1 Controls CaV3.2 Expression in DRG Neurons and Mechanical Sensitivity to Pain. Front Mol Neurosci. 2019;12:315 pubmed publisher
Li Q, Zhu Z, Lu J, Chao Y, Zhou X, Huang Y, et al. Sleep deprivation of rats increases postsurgical expression and activity of L-type calcium channel in the dorsal root ganglion and slows recovery from postsurgical pain. Acta Neuropathol Commun. 2019;7:217 pubmed publisher
Choi C, Souza I, Sanchez Arias J, Zamponi G, Arbour L, Swayne L. Ankyrin B and Ankyrin B variants differentially modulate intracellular and surface Cav2.1 levels. Mol Brain. 2019;12:75 pubmed publisher
Castagnola S, Delhaye S, Folci A, Paquet A, Brau F, Duprat F, et al. New Insights Into the Role of Cav2 Protein Family in Calcium Flux Deregulation in Fmr1-KO Neurons. Front Mol Neurosci. 2018;11:342 pubmed publisher
Zhang X, Patel D, Xu Q, VEENSTRA R. Differences in Functional Expression of Connexin43 and NaV1.5 by Pan- and Class-Selective Histone Deacetylase Inhibition in Heart. Int J Mol Sci. 2018;19: pubmed publisher
Newton J, Suman S, Akinfiresoye L, Datta K, Lovinger D, N GOUEMO P. Alcohol withdrawal upregulates mRNA encoding for CaV2.1-?1 subunit in the rat inferior colliculus. Alcohol. 2018;66:21-26 pubmed publisher
Varodayan F, Logrip M, Roberto M. P/Q-type voltage-gated calcium channels mediate the ethanol and CRF sensitivity of central amygdala GABAergic synapses. Neuropharmacology. 2017;125:197-206 pubmed publisher
Dorgans K, Salvi J, Bertaso F, Bernard L, Lory P, Doussau F, et al. Characterization of the dominant inheritance mechanism of Episodic Ataxia type 2. Neurobiol Dis. 2017;106:110-123 pubmed publisher
Fu S, Jeng C, Ma C, Peng Y, Lee C, Fang Y, et al. Ubiquitin Ligase RNF138 Promotes Episodic Ataxia Type 2-Associated Aberrant Degradation of Human Cav2.1 (P/Q-Type) Calcium Channels. J Neurosci. 2017;37:2485-2503 pubmed publisher
Valkova C, Liebmann L, Kramer A, Hübner C, Kaether C. The sorting receptor Rer1 controls Purkinje cell function via voltage gated sodium channels. Sci Rep. 2017;7:41248 pubmed publisher
Leo M, Schmitt L, Erkel M, Melnikova M, Thomale J, Hagenacker T. Cisplatin-induced neuropathic pain is mediated by upregulation of N-type voltage-gated calcium channels in dorsal root ganglion neurons. Exp Neurol. 2017;288:62-74 pubmed publisher
Tedeschi A, Dupraz S, Laskowski C, Xue J, Ulas T, Beyer M, et al. The Calcium Channel Subunit Alpha2delta2 Suppresses Axon Regeneration in the Adult CNS. Neuron. 2016;92:419-434 pubmed publisher
Liu C, Chang H, Tseng T, Lan C, Chen L, Youn S, et al. Redistribution of Cav2.1 channels and calcium ions in nerve terminals following end-to-side neurorrhaphy: ionic imaging analysis by TOF-SIMS. Histochem Cell Biol. 2016;146:599-608 pubmed
Tang W, Thevathasan J, Lin Q, Lim K, Kuroda K, Kaibuchi K, et al. Stimulation of Synaptic Vesicle Exocytosis by the Mental Disease Gene DISC1 is Mediated by N-Type Voltage-Gated Calcium Channels. Front Synaptic Neurosci. 2016;8:15 pubmed publisher
Dahimene S, Page K, Nieto Rostro M, Pratt W, D Arco M, Dolphin A. A CaV2.1 N-terminal fragment relieves the dominant-negative inhibition by an Episodic ataxia 2 mutant. Neurobiol Dis. 2016;93:243-56 pubmed publisher
Forostyak O, Butenko O, Anderova M, Forostyak S, Sykova E, Verkhratsky A, et al. Specific profiles of ion channels and ionotropic receptors define adipose- and bone marrow derived stromal cells. Stem Cell Res. 2016;16:622-34 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-CACNA1A (CaV2.1) Antibody (#ACC-001), (1:200).2. Anti-CACNA1A (CaV2.1) Antibody, preincubated with CACNA1A/Cav2.1 Blocking Peptide (#BLP-CC001).

image 2 :

Expression of CACNA1A in mouse cerebellum - Immunohistochemical staining of mouse cerebellum with Anti-CACNA1A (CaV2.1) Antibody (#ACC-001), (1:100). A. CACNA1A channel (red) appears in Purkinje cells (horizontal arrows) and is distributed diffusely in the molecular layer (Mol) including in astrocytic fibers (vertical arrows). B. Staining of astrocytic fibers with glial fibrillary acidic protein in the section demonstrates the location of astrocytic fibers in the molecular layer. C. Merged image of panels A and B.

image 3 :

Expression of CACNA1A in mouse cerebellum - Immunohistochemical staining ofmouse cerebellum withAnti-CACNA1A (CaV2.1)Antibody (#ACC-001) (1:100). A. CACNA1Achannel (red) appears in Purkinje cells (horizontal arrows) and is distributed diffusely in the molecular layer (Mol) including in astrocytic fibers (vertical arrows). B. Staining of astrocytic fibers with glial fibrillary acidic protein in the section demonstrates the location of astrocytic fibers in the molecular layer. C. Merged image of panels A and B.

product information

CAT :

ACC-001

SKU :

ACC-001-CF_0.2 ml

Product Name :

Anti-CACNA1A (CaV2.1) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P54282

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-CC001

Homology :

Mouse - 16/17 amino acid residues identical; human - 8/17 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular loop between domains II and III

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)PSSPERAPGREGPYGRE, corresponding to amino acid residues 865-881 of rat CACNA1A

Product Page - Scientific background :

Voltage-dependent Ca2+ channels (CaV channels) are pivotal players in many physiological roles such as secretion, contraction, migration and excitation.1The voltage-dependent calcium channels are composed of several subunits; α1, β, α2δ and γ. CaV channels were originally divided into six physiological types: L-, N-, P-, Q-, R-, and T-type.The CaV2.1 (formally named α1A) makes up the α1 poreforming subunit in P/Q-type Ca2+ channel family. It is expressed preferentially in the central nervous system where along with CaV2.2 is responsible for pre-synaptic Ca2+ influx and neurotransmitter release.1,2Mutations in CACNA1A (CaV2.1) have been shown to cause several neurological disorders among them are familial hemiplegic migraine, episodic ataxia type 2, and spinocerebellar ataxia type 6 (SCA6).1,3-5The involvement of CaV2.1 in synaptic transmission was assessed by using ω-Agatoxin IVA (#STA-500), a specific blocker of the CaV2.1 channel.6 The blocking sensitivity is dependent on the α subunit isoform and on the splice variant.7,8

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Voltage-dependent P/Q-type calcium channel subunit α1A, Brain calcium channel I, BI

Short Description :

A Rabbit Polyclonal Antibody to CACNA1A (CaV2.1) Channel

Long Description :

Anti-CACNA1A (CaV2.1) Antibody (#ACC-001) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize CaV2.1 from mouse, rat, and human samples.

Negative Control :

BLP-CC001

Positive Control :

Synonyms :

Voltage-dependent P/Q-type calcium channel subunit α1A, Brain calcium channel I, BI

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CACNA1A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP IHC ICC LCI

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage