Anti-TGR5 (GPBAR1) Antibody | Alomone Labs ABR-031 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TGR5 (GPBAR1) Antibody

catalog :

ABR-031

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 4), mouse liver (lanes 2 and 5) and rat spleen (lanes 3 and 6) lysates: - 1-3. Anti-TGR5 (GPBAR1) Antibody (#ABR-031), (1:200).4-6. Anti-TGR5 (GPBAR1) Antibody, preincubated with TGR5/GPBAR1 Blocking Peptide (#BLP-BR031).

image 2 :

Peptide (C)GDQRYTAPWRTAAQR corresponding to amino acid residues 282-296 of rat GPBAR1 (AccessionQ80T02). Intracellular C-terminus.

product information

CAT :

ABR-031

SKU :

ABR-031-CF_0.2 ml

Product Name :

Anti-TGR5 (GPBAR1) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q80T02

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-BR031

Homology :

Mouse - identical; human - 14/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)GDQRYTAPWRTAAQR, corresponding to amino acid residues 282-296 of rat GPBAR1

Product Page - Scientific background :

Bile acids are endogenous ligands for TGR5, a G-protein coupled receptor. Bile acids are important amphipathic biomolecules which can help absorption of dietary lipids and fat-soluble vitamins in the intestine. The TGR5 receptor is the first known G-protein coupled receptor specific for bile acids1.The coding sequence of the TGR5 gene contains 993 base pairs, encoding a protein of 330 amino acids. The receptor comprises seven transmembrane helices, three extracellular loops contributing to ligand binding, and three intracellular loops involved in mediating the signal to downstream signaling molecules2.TGR5 gene expression is widely distributed, including endocrine glands, adipocytes, muscles, immune organs, spinal cord, and the enteric nervous system3.Animal studies suggest that TGR5 activation influences energy production and thereby may be involved in obesity and diabetes. TGR5 activation also influences intestinal motility. TGR5 is now recognized as a potential target for the treatment of metabolic disorders, such as type 2 diabetes1. In addition, the ability of TGR5 to lower the levels of proinflammatory cytokines in monocytes has opened new insights into the modulatory role of bile acids in pathology where inflammatory processes play a central role, including colitis and atheroma development4,5.

Applications may also work in :

Supplier :

Alomone Labs

Target :

G-protein coupled bile acid receptor 1, M-BAR, GPR131, BG37

Short Description :

A Rabbit Polyclonal Antibody to G-Protein Coupled Bile Acid Receptor 1 (TGR5)

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope the rat G-protein coupled bile acid receptor 1. Anti-TGR5 (GPBAR1) Antibody (#ABR-031) can be used in western blot analysis. It has been designed to recognize TGR5 from mouse, rat and human samples.

Negative Control :

BLP-BR031

Positive Control :

Synonyms :

G-protein coupled bile acid receptor 1, M-BAR, GPR131, BG37

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GPBAR1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage