Anti-BAI2 (extracellular) Antibody | Alomone Labs ABR-022 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-BAI2 (extracellular) Antibody

catalog :

ABR-022

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Duman J, Mulherkar S, Tu Y, Erikson K, Tzeng C, Mavratsas V, et al. The adhesion-GPCR BAI1 shapes dendritic arbors via Bcr-mediated RhoA activation causing late growth arrest. elife. 2019;8: pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-BAI2 (extracellular) Antibody (#ABR-022), (1:200).3,4. Anti-BAI2 (extracellular) Antibody, preincubated with BAI2 (extracellular) Blocking Peptide (#BLP-BR022).

product information

CAT :

ABR-022

SKU :

ABR-022-CF_0.2 ml

Product Name :

Anti-BAI2 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8CGM1

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-BR022

Homology :

Rat, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)EPKVKTQWPRSADE, corresponding to amino acid residues 277-290 of mouse BAI2

Product Page - Scientific background :

Brain-specific angiogenesis inhibitor 2 (BAI2) is a member of the adhesion G-protein coupled receptor (GPCR) family. BAI2, and other members of its family, commonly have a long N-terminal extracellular region (ECR) containing a GPCR proteolysis site (GPS) adjacent to the seven-transmembrane (7TM) domain. The long N-terminus contains common structural domains, including thrombospondin type-I repeats (TSRs), epidermal growth factor (EGF)-like, leucine-rich repeats, lectin-like, immunoglobulin (Ig), and cadherins. BAI2 is composed of 521 amino acids and is mainly expressed in neurons.Although BAI2 is a GPCR, there are currently no reports showing evidence of any physiological ligands. Instead, BAI2 is activated by cleavage and dissociation of the ECR from its 7TM fragment, and functions as a GPCR. BAI2 is glycosylated and cleaved at the GPS by furin in the ER. The cleaved N-terminal ECR associates with the C-terminal 7TM fragment and they are translocated to the cell surface. The ECR associated with the 7TM fragment interacts with other membrane proteins or matrix proteins through the TSRs and dissociates from the 7TM fragment. The 7TM fragment which has released the associated ECR changes its conformation into the active state and activates the G protein-coupled signaling1.BAI2 is involved in the early stages of neovascularization of cerebral cortex after ischemia. Decreased levels of BAI2 induce increased VEGF expression mediated by GA-binding protein gamma (GABPγ) in cerebral ischemia and under normal conditions2.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Brain-specific angiogenesis inhibitor 2, Adhesion G protein-coupled receptor B2, ADGRB2

Short Description :

A Rabbit Polyclonal Antibody to BAI2

Long Description :

Anti-BAI2 (extracellular) Antibody (#ABR-022) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope, and can potentially be used for detecting the protein in living cells. It has been designed to recognize BAI2 from rat, mouse, and human samples.

Negative Control :

BLP-BR022

Positive Control :

Synonyms :

Brain-specific angiogenesis inhibitor 2, Adhesion G protein-coupled receptor B2, ADGRB2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ADGRB2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage