Anti-B1 Bradykinin Receptor (BDKRB1) (extracellular) Antibody | Alomone Labs ABR-013 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-B1 Bradykinin Receptor (BDKRB1) (extracellular) Antibody

catalog :

ABR-013

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysate (lanes 1 and 4), mouse brain lysate (lanes 2 and 5) and rat heart lysate (lanes 3 and 6):

image 2 :

Western blot analysis of mouse BV-2 microglia cell line lysate (lanes 1 and 5), mouse J774 macrophage cell line lysate (lanes 2 and 6), mouse M1 myeloid leukemia cell line lysate (lanes 3 and 7) and mouse Neuro-2a neuroblastoma cell line lysate (lanes 4 and 8):

image 3 :

Cell surface detection of B1 Bradykinin Receptor by indirect flow cytometry in live intact mouse BV-2 microglia cell line:

product information

CAT :

ABR-013

SKU :

ABR-013-CF_0.2 ml

Product Name :

Anti-B1 Bradykinin Receptor (BDKRB1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q61125

Applications :

IFC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-BR013

Homology :

Rat - 12 out of 13 amino acid residues identical Not recommended for human samples

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, 2nd loop.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)HEAWHFVRMVELN, corresponding to amino acid residues 202 - 214 of mouse BDKRB1

Product Page - Scientific background :

Kinins are small peptides rapidly produced following tissue injury that serve as important modulators of inflammation and pain. In the periphery, the actions of kinins include vasodilatation, increased vascular permeability, stimulation of immune cells, and induction of pain. Kinins in the central nervous system (CNS) appear to initiate a similar cascade of events leading to neural tissue damage as well as long lasting disturbances affecting blood-brain barrier function.1Kinins such as Bradykinin (BK), Lys-BK, desArg9-BK, and Lys-desArg9-BK exert their action via two distinct receptors: the B1 Bradykinin receptor (BKRB1) and the B2 Bradykinin receptor (BKRB2). The desArg9-BK and Lys-desArg9-BK peptides activate BKRB1 while BK and Lys-BK operate by activating BKRB2.2 Both BKRB1 and BKRB2 are members of the seven-transmembrane domain, G protein-coupled receptor (GPCR) superfamily and share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.Expression of BKRB1is inducible upon various types of tissue injury and by inflammatory mediators such as bacterial lipopolysaccharide (LPS) and cytokines. BKRB1 was long considered not to be expressed in healthy tissues. However, recent work has demonstrated a low level of expression of BKRB1 in the CNS of rodent and primates. In contrast, BKRB2 is constitutively expressed on various cell types.3 BKRB1 represents a potential therapeutic target for treatment of inflammatory disorders and cardiovascular diseases.

Applications may also work in :

IFC LCI WB

Supplier :

Alomone Labs

Target :

BK-1 receptor, BKRB1, B1R, BKR1

Short Description :

A Rabbit Polyclonal Antibody to B1 Bradykinin Receptor

Long Description :

Anti-B1 Bradykinin Receptor (BDKRB1) (extracellular) Antibody (#ABR-013) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and indirect flow cytometry applications. It has been designed to recognize B1 Bradykinin Receptor from mouse and rat samples. The antibody won’t recognize human samples.

Negative Control :

BLP-BR013

Positive Control :

Synonyms :

BK-1 receptor, BKRB1, B1R, BKR1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

BDKRB1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400-1:500

more info or order :

Alomone Labs product webpage