Anti-B2 Bradykinin Receptor (BDKRB2) Antibody | Alomone Labs ABR-012 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-B2 Bradykinin Receptor (BDKRB2) Antibody

catalog :

ABR-012

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Petrella C, Ciotti M, Nistico R, Piccinin S, Calissano P, Capsoni S, et al. Involvement of Bradykinin Receptor 2 in Nerve Growth Factor Neuroprotective Activity. Cells. 2020;9: pubmed publisher
Testa G, Mainardi M, Morelli C, Olimpico F, Pancrazi L, Petrella C, et al. The NGFR100W Mutation Specifically Impairs Nociception without Affecting Cognitive Performance in a Mouse Model of Hereditary Sensory and Autonomic Neuropathy Type V. J Neurosci. 2019;39:9702-9715 pubmed publisher
Severini C, Petrocchi Passeri P, Ciotti M, Florenzano F, Petrella C, Malerba F, et al. Nerve growth factor derivative NGF61/100 promotes outgrowth of primary sensory neurons with reduced signs of nociceptive sensitization. Neuropharmacology. 2017;117:134-148 pubmed publisher
Bitencourt R, Guerra de Souza A, Bicca M, Pamplona F, De Mello N, Passos G, et al. Blockade of hippocampal bradykinin B1 receptors improves spatial learning and memory deficits in middle-aged rats. Behav Brain Res. 2017;316:74-81 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysate: - 1. Anti-B2 Bradykinin Receptor (BDKRB2) Antibody (#ABR-012), (1:1000).2. Anti-B2 Bradykinin Receptor (BDKRB2) Antibody, preincubated with B2 Bradykinin Receptor/BDKRB2 Blocking Peptide (#BLP-BR012).

image 2 :

Expression of B2 bradykinin receptor in mouse spinal cord - Immunohistochemical staining of mouse spinal cord using Anti-B2 Bradykinin Receptor (BDKRB2) Antibody (#ABR-012). A. BKR2 (red) appears in several spinal cord neurons (triangles). B. Parvalbumin (green) appears in neurons. C. Merge of BKR2 and parvalbumin shows co-expression in several neurons (triangles) but not all (arrow in C). DAPI is used as the counterstain.

image 3 :

Peptide CGKRAIILETRQHR corresponding to amino acid residues 58-71 of human CX3CL1 (Accession P78423). Extracellular N-terminus.

product information

CAT :

ABR-012

SKU :

ABR-012-CF_0.2 ml

Product Name :

Anti-B2 Bradykinin Receptor (BDKRB2) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P30411

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-BR012

Homology :

Rat - 12/13 amino acid residues identical; mouse - 11/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

GKRFRKKSWEVYQG(C), corresponding to amino acid residues 336-349 of human B2R

Product Page - Scientific background :

Kinins are small peptides produced rapidly following tissue injury that serve as important modulators of inflammation and pain. In the periphery, the actions of kinins include vasodilatation, increased vascular permeability, stimulation of immune cells, and induction of pain. Kinins in the central nervous system (CNS) seem to initiate a similar cascade of events leading to neural tissue damage, as well as long lasting disturbances affecting blood-brain barrier function.1Kinins, such as Bradykinin (BK), Lys-BK, desArg9-BK, and Lys-desArg9-BK, exert their action via two distinct receptors: the B1 Bradykinin receptor (BKRB1) and the B2 Bradykinin receptor (BKRB2). BKRB2 is activated by BK and Lys-BK while desArg9-BK and Lys-desArg9-BK activate BKRB1.2 Activation of BKRB2 liberates mediators of vascular tone, fibrinolysis, and pain. BKRB2, which mediates most of the physiological effects of kinins, as well as BKRB1, represent potential therapeutic targets for treatment of inflammatory disorders and cardiovascular diseases.Both BKRB1 and BKRB2 are members of the seven-transmembrane domain, G protein-coupled receptor (GPCR) superfamily and share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxy terminus.BKRB2 is constitutively and widely expressed throughout the CNS and peripheral nervous system and on various cell types including endothelial cells, nerve fibers, leukocytes, and mast cells.3-5

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

B2R, BKR2, B2BKR, BKRB2, Kinin B2, BRADYB2

Short Description :

A Rabbit Polyclonal Antibody to B2 Bradykinin Receptor

Long Description :

Anti-B2 Bradykinin Receptor (BDKRB2) Antibody (#ABR-012) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize B2R from human, rat, and mouse samples.

Negative Control :

BLP-BR012

Positive Control :

Synonyms :

B2R, BKR2, B2BKR, BKRB2, Kinin B2, BRADYB2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

BDKRB2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:1000

more info or order :

Alomone Labs product webpage