Anti-B1 Bradykinin Receptor (BDKRB1) Antibody | Alomone Labs ABR-011 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-B1 Bradykinin Receptor (BDKRB1) Antibody

catalog :

ABR-011

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 5

Reference
Basuli D, Parekh R, White A, Thayyil A, Sriramula S. Kinin B1 Receptor Mediates Renal Injury and Remodeling in Hypertension. Front Med (Lausanne). 2021;8:780834 pubmed publisher
Parekh R, White A, Leffler K, Biancardi V, Eells J, Abdel Rahman A, et al. Hypothalamic kinin B1 receptor mediates orexin system hyperactivity in neurogenic hypertension. Sci Rep. 2021;11:21050 pubmed publisher
Parekh R, Sriramula S. Activation of Kinin B1R Upregulates ADAM17 and Results in ACE2 Shedding in Neurons. Int J Mol Sci. 2020;22: pubmed publisher
Parekh R, Robidoux J, Sriramula S. Kinin B1 Receptor Blockade Prevents Angiotensin II-induced Neuroinflammation and Oxidative Stress in Primary Hypothalamic Neurons. Cell Mol Neurobiol. 2020;40:845-857 pubmed publisher
Bitencourt R, Guerra de Souza A, Bicca M, Pamplona F, De Mello N, Passos G, et al. Blockade of hippocampal bradykinin B1 receptors improves spatial learning and memory deficits in middle-aged rats. Behav Brain Res. 2017;316:74-81 pubmed publisher

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 3) and heart (lanes 2 and 4) lysates: - 1-2. Anti-B1 Bradykinin Receptor (BDKRB1) Antibody (#ABR-011), (1:200).3-4. Anti-B1 Bradykinin Receptor (BDKRB1) Antibody, preincubated with B1 Bradykinin Receptor/BDKRB1 Blocking Peptide (#BLP-BR011).

image 2 :

Expression of B1 bradykinin receptor in rat CNS - Immunohistochemical staining of frozen rat brain section (A & B) and cerebellum (C) using Anti-B1 Bradykinin Receptor (BDKRB1) Antibody (#ABR-011), (1:50). BKR1 is expressed in neuronal cells of the amygdala (A) and hippocampus (CA1) (B) as well as in the axons of Purkinje cells in the cerebellum (arrowheads on C). Hoechst 33342 is used as the counterstain.

image 3 :

Expression of B1bradykinin receptorin rat CNS - Immunohistochemical staining of frozen rat brainsection (A B) and cerebellum (C) usingAnti-B1Bradykinin Receptor (BDKRB1) Antibody (#ABR-011) (1:50). BKR1is expressed in neuronal cells of the amygdala (A) and hippocampus (CA1) (B) as well as in the axons of Purkinje cells in the cerebellum (arrowheads on C). Hoechst 33342 is used as the counterstain.

product information

CAT :

ABR-011

SKU :

ABR-011-CF_0.2 ml

Product Name :

Anti-B1 Bradykinin Receptor (BDKRB1) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P97583

Applications :

IC IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-BR011

Homology :

Mouse - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KEAS RTR*SG GPKGS K, corresponding to amino acid residues 243-257 of rat B1R with replacement of cysteine 250 (C250) with serine (*S)

Product Page - Scientific background :

Kinins are small peptides rapidly produced following tissue injury that serve as important modulators of inflammation and pain. In the periphery, the actions of kinins include vasodilatation, increased vascular permeability, stimulation of immune cells, and induction of pain. Kinins in the central nervous system (CNS) appear to initiate a similar cascade of events leading to neural tissue damage as well as long lasting disturbances affecting blood-brain barrier function.1Kinins such as Bradykinin (BK), Lys-BK, desArg9-BK, and Lys-desArg9-BK exert their action via two distinct receptors: the B1 Bradykinin receptor (BKRB1) and the B2 Bradykinin receptor (BKRB2). The desArg9-BK and Lys-desArg9-BK peptides activate BKRB1 while BK and Lys-BK operate by activating BKRB2.2 Both BKRB1 and BKRB2 are members of the seven-transmembrane domain, G protein-coupled receptor (GPCR) superfamily and share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.Expression of BKRB1is inducible upon various types of tissue injury and by inflammatory mediators such as bacterial lipopolysaccharide (LPS) and cytokines. BKRB1 was long considered not to be expressed in healthy tissues. However, recent work has demonstrated a low level of expression of BKRB1 in the CNS of rodent and primates. In contrast, BKRB2 is constitutively expressed on various cell types.3 BKRB1 represents a potential therapeutic target for treatment of inflammatory disorders and cardiovascular diseases.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

B1R, BKR1, B1BKR, BKRB1, Kinin B1, BRADYB1

Short Description :

A Rabbit Polyclonal Antibody to B1 Bradykinin Receptor

Long Description :

Anti-B1 Bradykinin Receptor (BDKRB1) Antibody (#ABR-011) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize B1R from rat and mouse samples. The antibody may not recognize the receptor from human samples.

Negative Control :

BLP-BR011

Positive Control :

Synonyms :

B1R, BKR1, B1BKR, BKRB1, Kinin B1, BRADYB1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

BDKRB1

AB Specifictiy :

Might not recognize the human epitope.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:50

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage