product summary
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company name :
Alomone Labs
product type :
antibody
product name :
Anti-Bombesin Receptor 1 (NMBR) Antibody
catalog :
ABR-004
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
NA
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry
more info or order :
image
image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-Bombesin Receptor 1 (NMBR) Antibody (#ABR-004), (1:1000).3,4. Anti-Bombesin Receptor 1 (NMBR) Antibody, preincubated with Bombesin Receptor 1/NMBR Blocking Peptide (#BLP-BR004).
image 2 :

Expression of Bombesin receptor 1 in rat hypothalamus - Immunohistochemical staining of frozen rat hypothalamus sections using Anti-Bombesin Receptor 1 (NMBR) Antibody (#ABR-004), (1:200). A. Staining (red) appears in the neuropil near the ventricle (asterisk) and in neurons (triangles). Calbindin D28k staining (green) appears in neurons. C. Merge of (A) and (B) shows co-expression of BB1R and Calbindin in a few neurons (vertical arrow). DAPI is used as the counterstain.
image 3 :

Western blot analysis of human lung carcinoma NCI-H526 (lanes 1 and 3) and human prostate carcinoma PC-3 (lanes 2 and 4) cell line lysates: - 12.Anti-Bombesin Receptor 1(NMBR)Antibody (#ABR-004) (1:1000).34. Anti-Bombesin Receptor 1 (NMBR) Antibody preincubated with the control peptide antigen.
product information
CAT :
ABR-004
SKU :
ABR-004-CF_0.2 ml
Product Name :
Anti-Bombesin Receptor 1 (NMBR) Antibody
Group Type :
Antibodies
Product Type :
Antibodies
Clonality :
Polyclonal
Accession :
P28336
Applications :
IC IF IHC WB
Reactivity :
Human Rat Mouse
Host :
Rabbit
Blocking Peptide :
BLP-BR004
Homology :
Mouse, dog - identical; rat - 15/16 amino acid residues identical
Formulation :
PBS pH7.4
isotype :
Rabbit IgG
Peptide confirmation :
Confirmed by amino acid analysis and mass spectrometry
Reconstitution :
0.2 ml double distilled water (DDW).
Antibody Concentration After Reconstitut ... :
1 mg/ml
Storage After Reconstitution :
The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preservative :
No Preservative
Immunogen Location :
3rd intracellular loop
Label :
Unconjugated
Storage Before Reconstitution :
The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
Shipping and storage :
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
immunogen source species :
Human
Sequence :
(C)KSAHNLPGEYNEHTKK, corresponding to amino acid residues 241-256 of human BB1R
Product Page - Scientific background :
Bombesin receptor 1 (BB1) is a member of a family of receptors that binds the 14 amino acid peptide bombesin. Bombesin was initially isolated from frog skin and it was later established that mammals express endogenous bombesin-like peptides such as gastrin-releasing peptide (GRP) and neuromedin B (NMB), 27 and 10 amino acid homologues, respectively.1BB1 is the preferred receptor for NMB (therefore it is also known as NMB receptor) while BB2 is the preferred receptor for GRP.2 BB3 was the third member of the bombesin family of receptors to be cloned based upon its sequence similarity to BB1 and BB2.1 The affinity of BB3 for bombesin is lower than that of the BB1 and BB2 receptors, as are its affinities for NMB and GRP. This suggests that the endogenous mammalian ligand for the BB3 receptor remains to be identified.All three bombesin receptors are members of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. BB1 is coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (InsP3), intracellular Ca2+ mobilization, and cell growth.1BB1 is expressed in the brain where it likely mediates the inhibition of food intake and regulates the stress and fear response. BB1 and its ligand NMB are expressed in the pituitary where they participate in the regulation of thyroid-stimulating hormone (TSH) secretion in both autocrine and paracrine fashions.3 In the periphery, BB1 is expressed in the gastrointestinal and urogenital smooth muscle but its physiological function in these tissues is unclear.Finally, BB1 is over-expressed in several human tumors especially colon and non-small lung cancer where it acts as a growth factor receptor inducing tumor growth. BB1 is considered a potential target for the development of both diagnostics and anti-cancer therapies.4
Applications may also work in :
IC IF IHC WB
Supplier :
Alomone Labs
Target :
BB1R, Neuromedin B receptor, Neuromedin-B-preferring bombesin receptor
Short Description :
A Rabbit Polyclonal Antibody to Bombesin Receptor 1/Neuromedin B Receptor
Long Description :
Alomone Labs is pleased to offer a highly specific antibody directed against a well conserved epitope located in the 3rd intracellular loop of the human BB1 receptor (neuromedin B receptor). Anti-Bombesin Receptor 1 (NMBR) Antibody (#ABR-004) can be used in western blot and immunocytochemical applications, and will recognize BB1R from human, rat, mouse, and dog samples.
Negative Control :
BLP-BR004
Positive Control :
NA
Synonyms :
BB1R, Neuromedin B receptor, Neuromedin-B-preferring bombesin receptor
Lead Time :
1-2 Business Days
Country of origin :
Israel/IL
Applications key :
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Specifictiy :
NMBR
Form :
Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Comment :
Contact Alomone Labs for technical support and product customization
Species reactivity key :
H- Human, M- Mouse, R- Rat
Is Toxin :
No
Purity :
Affinity purified on immobilized antigen.
UNSPSC :
41116161
Clone :
NA
Standard quality control of each lot :
Western blot analysis
Antigen preadsorption control :
1 µg peptide per 1 µg antibody
Application Dilutions: Immunohistochemis ... :
1:200
Application Dilutions: Western blot wb :
1:1000
more info or order :
company information

Alomone Labs
Jerusalem BioPark (JBP), Hadassah Ein Kerem
P.O. Box 4287
Jerusalem 9104201
P.O. Box 4287
Jerusalem 9104201
info@alomone.com
http://www.alomone.com972 2 531 8002
headquarters: Israel
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