antibody

Anti-Bombesin Receptor 1 (NMBR) Antibody

ABR-004

polyclonal

domestic rabbit

nonconjugated

NA

human, mouse, rat

western blot, immunohistochemistry, immunocytochemistry

ABR-004-CF_0.2 ml

Anti-Bombesin Receptor 1 (NMBR) Antibody

Antibodies

Antibodies

Polyclonal

P28336

IC IF IHC WB

Human Rat Mouse

Rabbit

BLP-BR004

Mouse, dog - identical; rat - 15/16 amino acid residues identical

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

3rd intracellular loop

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Human

(C)KSAHNLPGEYNEHTKK, corresponding to amino acid residues 241-256 of human BB1R

Bombesin receptor 1 (BB1) is a member of a family of receptors that binds the 14 amino acid peptide bombesin. Bombesin was initially isolated from frog skin and it was later established that mammals express endogenous bombesin-like peptides such as gastrin-releasing peptide (GRP) and neuromedin B (NMB), 27 and 10 amino acid homologues, respectively.1BB1 is the preferred receptor for NMB (therefore it is also known as NMB receptor) while BB2 is the preferred receptor for GRP.2 BB3 was the third member of the bombesin family of receptors to be cloned based upon its sequence similarity to BB1 and BB2.1 The affinity of BB3 for bombesin is lower than that of the BB1 and BB2 receptors, as are its affinities for NMB and GRP. This suggests that the endogenous mammalian ligand for the BB3 receptor remains to be identified.All three bombesin receptors are members of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. BB1 is coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (InsP3), intracellular Ca2+ mobilization, and cell growth.1BB1 is expressed in the brain where it likely mediates the inhibition of food intake and regulates the stress and fear response. BB1 and its ligand NMB are expressed in the pituitary where they participate in the regulation of thyroid-stimulating hormone (TSH) secretion in both autocrine and paracrine fashions.3 In the periphery, BB1 is expressed in the gastrointestinal and urogenital smooth muscle but its physiological function in these tissues is unclear.Finally, BB1 is over-expressed in several human tumors especially colon and non-small lung cancer where it acts as a growth factor receptor inducing tumor growth. BB1 is considered a potential target for the development of both diagnostics and anti-cancer therapies.4

IC IF IHC WB

Alomone Labs

BB1R, Neuromedin B receptor, Neuromedin-B-preferring bombesin receptor

A Rabbit Polyclonal Antibody to Bombesin Receptor 1/Neuromedin B Receptor

Alomone Labs is pleased to offer a highly specific antibody directed against a well conserved epitope located in the 3rd intracellular loop of the human BB1 receptor (neuromedin B receptor). Anti-Bombesin Receptor 1 (NMBR) Antibody (#ABR-004) can be used in western blot and immunocytochemical applications, and will recognize BB1R from human, rat, mouse, and dog samples.

BLP-BR004

NA

BB1R, Neuromedin B receptor, Neuromedin-B-preferring bombesin receptor

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

NMBR

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis

1 µg peptide per 1 µg antibody

1:200

1:1000