Anti-Bombesin Receptor 3 (extracellular) Antibody | Alomone Labs ABR-003 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Bombesin Receptor 3 (extracellular) Antibody

catalog :

ABR-003

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-Bombesin Receptor 3 (extracellular) Antibody (#ABR-003), (1:200).2. Anti-Bombesin Receptor 3 (extracellular) Antibody, preincubated with Bombesin Receptor 3 (extracellular) Blocking Peptide (#BLP-BR003).

image 2 :

Expression of BRS3 in rat testes - Immunohistochemical staining of paraffin embedded rat testes section using Anti-Bombesin Receptor 3 (extracellular) Antibody (#ABR-003), (1:50). The area of the efferent ductules near the epididymis is shown. Intense stain (brown) is specific for the pseudostratified epithelium of the efferent ductules. DAB is used for the color reaction. H&E is used as the counterstain.

image 3 :

Cell surface detection of BRS3in live intact human promyelocytic leukemia HL-60 cells: - ___Unstained cells + goat-anti-rabbit-FITC.___Cells +Anti-Bombesin Receptor 3 (extracellular) Antibody(#ABR-003) (5 g) + goat-anti-rabbit-FITC.

product information

CAT :

ABR-003

SKU :

ABR-003-CF_0.2 ml

Product Name :

Anti-Bombesin Receptor 3 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P32247

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-BR003

Homology :

Rat, mouse - 13/16 amino acids residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)SNVYTFRDPNKNMTFE, corresponding to amino acid residues 186-201 of human BRS3

Product Page - Scientific background :

Bombesin receptor 3 (BB3) is a member of a family of receptors that binds the 14 amino acid peptide bombesin. Bombesin was first isolated from frog skin and it was later established that mammalians express endogenous bombesin like peptides such as gastrin-releasing peptide (GRP) a 27 amino acid homologue and neuromedin B (NMB) a 10 amino acid homologue.1BB1 is the preferred receptor for NMB while BB2 is the preferred receptor for GRP.BB3 was the third member of the bombesin receptors family to be cloned based in its sequence similarity to BB1 and BB2.2 The affinity of BB3 for bombesin is lower than that of the BB1 and BB2 receptors as is its affinity for NMB and GRP. This suggests that the mammalian endogenous ligand for the BB3 receptor remains to be identified.All three bombesin receptors are members of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. BB3 is coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (InsP3), intracellular Ca2+ mobilization and cell growth.3The physiological function of the BB3 receptor is not clear. However, studies with BB3 receptor deficient mice have shown that the mice develop mild obesity associated with hypertension and reduced metabolic rate.4,5Finally, BB3 receptor expression has been identified in several human tumors most notably lung. BB3 is therefore considered a potential target for the development of both diagnostics and anti-cancer therapies.6

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

BB3R, BRS3

Short Description :

A Rabbit Polyclonal Antibody to Bombesin Receptor 3

Long Description :

Alomone Labs is pleased to present a highly specific antibody directed against a well conserved epitope of the human BB3 receptor. Anti-Bombesin Receptor 3 (extracellular) Antibody (#ABR-003) can be used in western blot analysis, as well as immunohistochemical and flow cytometry applications, and will recognize BRS3 from rat and mouse samples.

Negative Control :

BLP-BR003

Positive Control :

Synonyms :

BB3R, BRS3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

BRS3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:50

Application Dilutions: Western blot wb :

1:200-1:500

more info or order :

Alomone Labs product webpage