Anti-Bombesin Receptor 2 (GRPR) (extracellular) Antibody | Alomone Labs ABR-002 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Bombesin Receptor 2 (GRPR) (extracellular) Antibody

catalog :

ABR-002

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-Bombesin Receptor 2 (GRPR) (extracellular) Antibody (#ABR-002), (1:500).3,4. Anti-Bombesin Receptor 2 (GRPR) (extracellular) Antibody, preincubated with Bombesin Receptor 2/GRPR (extracellular) Blocking Peptide (#BLP-BR002).

image 2 :

Expression of Bombesin receptor 2 in human colon - Immunohistochemical staining of paraffin-embedded human colon using Anti-Bombesin Receptor 2 (GRPR) (extracellular) Antibody (#ABR-002), (1:50). (A and B) Human colon showing malignant growth. Staining is specific for epithelium-derived malignant cells. (C and D) Normal colon, staining is specific for absorptive epithelial cells in the crypts of Lieberkuhn. Histofine (pink) is used for the color reaction. Hematoxilin is used as the counterstain.

image 3 :

Western blot analysisof human DU-145 (lanes 1 and 3) and PC-3 (lanes 2 and 4) prostate carcinoma cell lines: - 12.Anti-Bombesin Receptor 2 (GRPR) (extracellular)Antibody (#ABR-002) (1:200).34. Anti-Bombesin Receptor 2(GRPR) (extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

ABR-002

SKU :

ABR-002-CF_0.2 ml

Product Name :

Anti-Bombesin Receptor 2 (GRPR) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P30550

Applications :

IC IF IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-BR002

Homology :

Rat, mouse, dog - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)RSYHYSEVDTSMLH, corresponding to amino acid residues 287-300 of human BB2R

Product Page - Scientific background :

Bombesin receptor 2 (BB2) is a member of a family of receptors that binds the 14 amino acid peptide, bombesin. Bombesin was first isolated from frog skin and it was later established that mammals express endogenous bombesin-like peptides such as gastrin-releasing peptide (GRP; a 27 amino acid homologue) and neuromedin B (NMB; a 10 amino acid homologue).BB2 is the preferred receptor for GRP and hence it is also referred to as GRP receptor or GRP-R.1 BB1 is the preferred receptor for NMB and therefore it is also known as NMB-R. A third member (called BB3) of the bombesin receptor family has been recognized but the endogenous mammalian ligand for this receptor remains to be identified.All three bombesin receptors are members of the seven-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. BB2 is coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (InsP3), intracellular Ca2+ mobilization, and cell growth.2GRP (through its binding to BB2) plays an essential role in the release of hormones in the intestinal tract, the regulation of smooth muscle contraction, and the secretion of pancreatic enzymes.2 In the brain, BB2 has been implicated in regulating feeding behavior, thermoregulation, and memory formation.3Finally, BB2 is overexpressed in several human tumors including breast, prostate, and lung, where it acts as a growth factor receptor inducing tumor growth. BB2 is considered a potential target for the development of both diagnostics and anti-cancer therapies.4

Applications may also work in :

IC IF IHC LCI WB

Supplier :

Alomone Labs

Target :

BB2R, Gastrin-releasing peptide receptor, GRP-preferring bombesin receptor

Short Description :

A Rabbit Polyclonal Antibody to Bombesin Receptor 2/Gastrin-releasing Peptide Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located in the 3rd extracellular loop of the human BB2 receptor (gastrin-releasing peptide receptor). Anti-Bombesin Receptor 2 (extracellular) (GRPR) Antibody (#ABR-002) can be used in western blot analysis, as well as immunocytochemical and immunohistochemical applications, and will recognize BB2R from human, rat, mouse, and dog samples.

Negative Control :

BLP-BR002

Positive Control :

Synonyms :

BB2R, Gastrin-releasing peptide receptor, GRP-preferring bombesin receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GRPR

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:50

Application Dilutions: Western blot wb :

1:200-1:500

more info or order :

Alomone Labs product webpage