antibody

Anti-Bestrophin-1 (extracellular) Antibody

ABC-001

polyclonal

domestic rabbit

nonconjugated

NA

human, mouse, rat

western blot, immunohistochemistry

ABC-001-CF_0.2 ml

Anti-Bestrophin-1 (extracellular) Antibody

Antibodies

Antibodies

Polyclonal

O88870

IFC IHC LCI WB

Human Rat Mouse

Rabbit

BLP-BC001

Rat - 10/12 amino acid residues identical; human - 9/12 amino acid residues identical

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

3rd extracellular loop

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Mouse

(C)NPNKDYPGHEMD, corresponding to amino acid residues 259-270 of mouse Bestrophin-1

Mammalian Cl- channels can be broadly classified into four different families: voltage-dependent Cl- channels (CLCs), the cystic fibrosis transmembrane conductance regulator (CFTR), ligand-gated Cl- channels (γ-aminobutyric acid (GABA)) and glycine channels) and Ca2+-activated Cl- channels (Bestrophin and Anoctamin channels).Bestrophins were first found by genetic linkage of human-Bestrophin-1 (hBest1) to a juvenile form of macular degeneration called Best vitelliform macular dystrophy (BVMD)1,2. BVMD is mainly electrophysiologically characterized by a decrease in the light peak and physiologically by the thinning of the retina layer which eventually leads to the loss of central vision3. To date Bestrophin 1-4 have been identified, although Bestrophin-3 and Bestrophin-4 have been observed only at the RNA level3. In addition, splice variants of some of these Ca2+-activated Cl- channels (CaCCs) have also been detected2,4,5. CaCCs are known to be involved in the regulation of olfaction, taste, phototransduction, and excitability in the nervous system. Recently, Bestrophin-1 was shown to be functionally expressed in astrocytes in both primary cell culture and in situ6. Bestrophin-1 is also detected in retina, brain, spinal cord and testes7.Two different topologies for Bestrophin-1 have been proposed. The first, the preferred structure, proposes that six hydrophobic domains span the membrane8, while the second suggests that there are only four membrane-spanning domains9. Bestrophin-1, along with its counterparts, is activated by intracellular Ca2+. A recent study demonstrated that Bestrophin-1 indeed binds Ca2+ and by mutating specific residues, showed which amino acid residues are essential for binding Ca2+ 10, providing additional evidence that Bestrophin-1 is activated by direct binding of Ca2+ to the channel11,12.Bestrophin-1 has been found to release glutamate from astrocytes and is located at microdomains near synapses13.

IFC IHC LCI WB

Alomone Labs

BEST1, Vitelliform macular dystrophy protein 2, VMD2, BMD1

A Rabbit Polyclonal Antibody to Bestrophin-1 Channel

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of mouse Bestrophin-1. Anti-Bestrophin-1 (extracellular) Antibody (#ABC-001) can be used in western blot, immunohistochemistry and indirect flow cytometry applications. It has been designed to recognize bestrophin-1 channel from mouse, rat and human samples.

BLP-BC001

NA

BEST1, Vitelliform macular dystrophy protein 2, VMD2, BMD1

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

BEST1

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

ICC

NA

Western blot analysis

1 µg peptide per 1 µg antibody

1:100

1:200