Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody | Alomone Labs AAR-043 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody

catalog :

AAR-043

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
van Oostrum M, Campbell B, Seng C, Müller M, Tom Dieck S, Hammer J, et al. Surfaceome dynamics reveal proteostasis-independent reorganization of neuronal surface proteins during development and synaptic plasticity. Nat Commun. 2020;11:4990 pubmed publisher
Saeed S, Bonnefond A, Tamanini F, Mirza M, Manzoor J, Janjua Q, et al. Loss-of-function mutations in ADCY3 cause monogenic severe obesity. Nat Genet. 2018;50:175-179 pubmed publisher

image

image 1 :

Western blot analysis of rat lung (lanes 1 and 4), rat brain (lanes 2 and 5) and hippocampus (lanes 3 and 6) lysates: - 1-3. Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody (#AAR-043), (1:200).4-6. Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody, preincubated with Adenylate Cyclase 3/AC3 (extracellular) Blocking Peptide (#BLP-AR043).

image 2 :

Expression of Adenylate cyclase type III in mouse hippocampal neurons - Immunohistochemical staining of mouse hippocampal sections using Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody (#AAR-043), (1:400), (red). A. AC3 staining of neurons. B. Staining with mouse anti-NeuN (green), as a neuronal marker on the same section. C. Merge of A and B demonstrates “primary cilia” - thin rod-like extensions from neurons in the pyramidal layer of the hippocampus.

image 3 :

Expression of Adenylate cyclase type IIIin rat U-87 MG cells - Cell surface detection ofAdenylate cyclase type III in intact living rat U-87 MG cells. A. Extracellular staining of cells usingAnti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody(#AAR-043) (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Merge of A with live view of the cells.

product information

CAT :

AAR-043

SKU :

AAR-043-CF_0.2 ml

Product Name :

Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P21932

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-AR043

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KHVADEMLKDMKKDE, corresponding to amino acid residues 285-299 of rat ADCY3

Product Page - Scientific background :

Adenylyl cyclases (ACs) are a family of enzymes that synthesize cAMP, upon stimulation. cAMP is an important second messenger, which regulates carbohydrate, lipid, protein, and nucleic acid metabolism as well as synaptic transmission, ion channel function, and transcription in neurons1. There are nine closely related isoforms of ACs (AC1-9) in mammals. All AC isoforms share a primary structure consisting of two hydrophobic regions which comprise six transmembrane helices and three large cytoplasmic domains. The basic catalytic unit of a membrane-bound AC molecule consists of the C1a and C2 domains. The N-terminus domains of ACs are highly variable, and might play a regulatory role2.Adenylyl cyclase type 3 (AC3) is widely expressed in the olfactory tissue, human cell line HEK-293, bovine brain, spinal cord, adrenal medulla, adrenal cortex, heart atrium, aorta, lung and retina3.Several studies have suggested that AC3 may play an important role in the regulation of glucose metabolism and body weight. It was found that overexpression of AC3 mRNA is involved in pathophysiological metabolic processes, such as type 2 diabetes4. In addition, a recent study demonstrated the association of AC3 with gastric cancer as well as its tumorigenic potentials5.Furthermore, AC3-knockout mice showed peripheral and behavioral anosmia6.

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Adenylate cyclase type 3, Adenylate cyclase type III, AC-III, ATP pyrophosphate-lyase 3, Adenylate cyclase olfactive type, Adenylyl cyclase 3, ADCY3

Short Description :

A Rabbit Polyclonal Antibody to Adenylate Cyclase Type III

Long Description :

Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody (#AAR-043) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and live cell imaging applications. The antibody recognizes an extracellular epitope and thus is ideal for detecting ADCY3 in living cells. It has been designed to recognize ADCY3 from human, rat and mouse samples.

Negative Control :

BLP-AR043

Positive Control :

Synonyms :

Adenylate cyclase type 3, Adenylate cyclase type III, AC-III, ATP pyrophosphate-lyase 3, Adenylate cyclase olfactive type, Adenylyl cyclase 3, ADCY3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ADCY3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

LCI

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage