Anti-Adenylate Cyclase 3 (AC3) (extracellular)-ATTO Fluor-488 Antibody | Alomone Labs AAR-043-AG product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Adenylate Cyclase 3 (AC3) (extracellular)-ATTO Fluor-488 Antibody

catalog :

AAR-043-AG

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 488

clone name :

reactivity :

human, mouse, rat

application :

immunocytochemistry, flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Expression of Adenylate cyclase type IIIin rat U-87 MG cells - Cell surface detection ofAdenylate cyclase type III in intact living rat U-87 MG cells. A. Extracellular staining of cells usingAnti-Adenylate Cyclase 3 (AC3) (extracellular)-ATTO-488 Antibody(#AAR-043-AG) (1:25) (green). B. Merge of A with live view of the cells.

image 2 :

Western blot analysis of mouse BV-2 microglia cell line lysate (lanes 1 and 3) and human THP-1 monocytic leukemia cell line lysate (lanes 2 and 4): - 12. Anti-GPR34 (extracellular) Antibody (#AGR-055) (1:200). 34. Anti-GPR34 (extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

AAR-043-AG

SKU :

AAR-043-AG_50 mcl

Product Name :

Anti-Adenylate Cyclase 3 (AC3) (extracellular)-ATTO Fluor-488 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P21932

Applications :

FC IC IF LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-AR043

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

3rd extracellular loop

Label :

ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KHVADEMLKDMKKDE, corresponding to amino acid residues 285-299 of rat ADCY3

Product Page - Scientific background :

Adenylyl cyclases (ACs) are a family of enzymes that synthesize cAMP, upon stimulation. cAMP is an important second messenger, which regulates carbohydrate, lipid, protein, and nucleic acid metabolism as well as synaptic transmission, ion channel function, and transcription in neurons1. There are nine closely related isoforms of ACs (AC1-9) in mammals. All AC isoforms share a primary structure consisting of two hydrophobic regions which comprise six transmembrane helices and three large cytoplasmic domains. The basic catalytic unit of a membrane-bound AC molecule consists of the C1a and C2 domains. The N-terminus domains of ACs are highly variable, and might play a regulatory role2.Adenylyl cyclase type 3 (AC3) is widely expressed in the olfactory tissue, human cell line HEK-293, bovine brain, spinal cord, adrenal medulla, adrenal cortex, heart atrium, aorta, lung and retina3.Several studies have suggested that AC3 may play an important role in the regulation of glucose metabolism and body weight. It was found that overexpression of AC3 mRNA is involved in pathophysiological metabolic processes, such as type 2 diabetes4. In addition, a recent study demonstrated the association of AC3 with gastric cancer as well as its tumorigenic potentials5.Furthermore, AC3-knockout mice showed peripheral and behavioral anosmia6.

Applications may also work in :

FC IC IF LCI

Supplier :

Alomone Labs

Target :

Adenylate cyclase type 3, Adenylate cyclase type III, AC-III, ATP pyrophosphate-lyase 3, Adenylate cyclase olfactive type, Adenylyl cyclase 3, ADCY3

Short Description :

A Rabbit Polyclonal Antibody to Adenylate Cyclase Type III Conjugated to the Fluorescent Dye ATTO-488

Long Description :

Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody (#AAR-043) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and live cell imaging applications. The antibody recognizes an extracellular epitope and thus is ideal for detecting ADCY3 in living cells. It has been designed to recognize ADCY3 from human, rat and mouse samples. Anti-Adenylate Cyclase 3 (AC3) (extracellular)-ATTO Fluor-488 Antibody (#AAR-043-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-Adenylate Cyclase 3 (AC3) (extracellular)-ATTO Fluor-488 Antibody has been tested in live cell imaging applications and is especially suited for experiments requiring simultaneous labeling of different markers.

Negative Control :

RIC-001-AG

Positive Control :

Synonyms :

Adenylate cyclase type 3, Adenylate cyclase type III, AC-III, ATP pyrophosphate-lyase 3, Adenylate cyclase olfactive type, Adenylyl cyclase 3, ADCY3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ADCY3

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #AAR-043), and immunocytochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage