Anti-Adenosine A3 Receptor (extracellular)-FITC Antibody | Alomone Labs AAR-035-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Adenosine A3 Receptor (extracellular)-FITC Antibody

catalog :

AAR-035-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

FITC

clone name :

reactivity :

mouse

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of Adenosine A3 Receptor by direct flow cytometry in live intact mouse WEHI-231 B lymphocyte cell line: Cell surface detection of Adenosine A3 Receptor by direct flow cytometry in live intact mouse WEHI-231 B lymphocyte cell line: Black line: Cells. Red line: Cells + Rabbit IgG isotype control-FITC. Green line: Cells + Anti-Adenosine A3 Receptor (extracellular)-FITC Antibody (#AAR-035-F), (2.5µg).

product information

CAT :

AAR-035-F

SKU :

AAR-035-F_15 mcl

Product Name :

Anti-Adenosine A3 Receptor (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q61618

Applications :

FC LCI

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-AR035

Homology :

Rat - 10 out of 13 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, 2nd loop.

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)GWNRKATLASSQN, corresponding to amino acid residues 149 - 161 of mouse ADORA3

Product Page - Scientific background :

Adenosine is an endogenous nucleoside generated locally in tissues under conditions of hypoxia, ischemia, or inflammation. It modulates a variety of physiological functions in many tissues including the brain and heart.1,2 Adenosine exerts its action via four specific adenosine receptors (also named P1 purinergic receptors): Adenosine A1 Receptor (A1AR), Adenosine A2A Receptor (A2AAR), Adenosine A2B Receptor (A2BAR), and Adenosine A3 Receptor (A3AR). The various adenosine receptors can be distinguished on the basis of their distinct molecular structures, distinct tissue distributions, and differential selectivity for adenosine analogs.1-4 All are integral membrane proteins and are members of the G protein-coupled receptor superfamily. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, a cytoplasmic carboxyl terminus, and a third intracellular loop important in binding G proteins.1-3Expression of A3AR has been reported in the brain, kidney, liver, and heart. It plays a role in modulation of cerebral ischemia, asthma, and cell growth. In addition, recent studies have established a cardioprotective role for A3AR.5 Expression of A3AR was reported to be elevated in cancerous tissues as well as in auto-immune inflammatory diseases.6,7 A patent has been filed for the use of A3AR and A3AR agonist as a diagnostic marker for therapeutic treatments of cancer and other diseases.

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

ADORA3, A3AR, Adenosine Receptor A3

Short Description :

A Rabbit Polyclonal Antibody to Adenosine A3 Receptor (extracellular) conjugated to the fluorescent dye FITC

Long Description :

Anti-Adenosine A3 Receptor (extracellular) Antibody (#AAR-035) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and flow cytometry applications. It has been designed to recognize Adenosine A3 from mouse and rat samples. The antibody will not recognize the receptor from human samples. Anti-Adenosine A3 Receptor (extracellular)-FITC Antibody (#AAR-035-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. The antibody will not recognize the receptor from human samples.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

ADORA3, A3AR, Adenosine Receptor A3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ADORA3

AB Specifictiy :

The antibody will not recognize adenosine A3 receptor from human samples.

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #AAR-035), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage