Anti-C5aR2 (extracellular) Antibody | Alomone Labs AAR-034 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-C5aR2 (extracellular) Antibody

catalog :

AAR-034

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4): - 1-2. Anti-C5aR2 (extracellular) Antibody (#AAR-034), (1:400). 3-4. Anti-C5aR2 (extracellular) Antibody, preincubated with C5aR2 (extracellular) Blocking Peptide (BLP-AR034).

image 2 :

Expression of C5aR2 in rat hippocampus. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-C5aR2 (extracellular) Antibody (#AAR-034), (1: 300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the hippocampal CA1 region, showed C5aR2 immunoreactivity (green) in glial profiles (arrows). B. Pre-incubation of the antibody with C5aR2 (extracellular) Blocking Peptide (BLP-AR034), suppressed staining. Cell nuclei are stained with DAPI (blue). P = pyramidal layer, SO = stratum oriens, SR = stratum radiatum.

product information

CAT :

AAR-034

SKU :

AAR-034-CF_0.2 ml

Product Name :

Anti-C5aR2 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8BW93

Applications :

IFC IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-AR034

Homology :

Mouse - 14 out of 15 amino acid residues identical; Rat - 13 out of 15 amino acid residues identical Will not recognize Human C5aR2

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, 2nd loop.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)RRLLQEHYPPRLV(S)G, corresponding to amino acid residues 179 - 193 of mouse C5aR2

Product Page - Scientific background :

The complement system is a vital component of the innate immune system that play an important role in the immune responses to infectious organisms and tissue damages 1. The activation of the complement system is carried out through a proteolytic enzymes cascade generating various peptides and fragments 2.One of the most important component of the complement system is a protein named complement protein C5 3. Upon proteolytic cleavage of C5 two peptides are generated, C5a and C5b 4. C5a is known to be the most potent chemotactic factor in cells and responsible for the directed migration of cells involved in the immune response to infectious organisms and tissue damages 5. There are two different C5a receptors, C5aR1 (C5aR, CD88) and C5aR2 (C5L2, GPR77). C5aR1 is a member of the G-protein coupled receptors (GPCRs), and known to mediate the vast majority of C5a biological activity 6. In contrast C5aR2 does not exhibit any functional coupling to G proteins and it exact mechanism of action remains controversial 7. Expression of C5aR2 can be found in various tissues such as, bone marrow, spleen, lung and in immune cells such as myeloid cells and T cells 3.

Applications may also work in :

IFC IHC WB

Supplier :

Alomone Labs

Target :

Complement Component 5a Receptor 2, C5L2, C5a Anaphylatoxin Chemotactic Receptor 2, G Protein-Coupled Receptor 77, GPR77

Short Description :

A Rabbit Polyclonal antibody to C5aR2 (extracellular).

Long Description :

Anti-C5aR2 (extracellular) Antibody (#AAR-034) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize C5aR2 from mouse and rat samples. The antibody will not recognize C5aR2 from human samples.

Negative Control :

BLP-AR034

Positive Control :

Synonyms :

Complement Component 5a Receptor 2, C5L2, C5a Anaphylatoxin Chemotactic Receptor 2, G Protein-Coupled Receptor 77, GPR77

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

C5AR2

AB Specifictiy :

Will not recognize Human C5aR2.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage