Anti-Human C5aR2 (extracellular) Antibody | Alomone Labs AAR-033 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Human C5aR2 (extracellular) Antibody

catalog :

AAR-033

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of human MEG-O1 chronic myelogenous leukemia cell lines lysate (lanes 1 and 3) and human K562 erythroleukemia cell line lysate (lanes 2 and 4): - 1,2. Anti-Human C5aR2 (extracellular) Antibody (#AAR-033), (1:400).3,4. Anti-Human C5aR2 (extracellular) Antibody, preincubated with Human C5aR2 (extracellular) Blocking Peptide (#BLP-AR033).

product information

CAT :

AAR-033

SKU :

AAR-033-CF_0.2 ml

Product Name :

Anti-Human C5aR2 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9P296

Applications :

IFC LCI WB

Reactivity :

Human

Host :

Rabbit

Blocking Peptide :

BLP-AR033

Homology :

Human only

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)DSVSYEYGDYSDLSD, corresponding to amino acid residues 4-18 of human C5a anaphylatoxin chemotactic receptor 2

Product Page - Scientific background :

Complement fragment C5a is a pro-inflammatory polypeptide produced during activation of the complement cascade of serum proteins in response to foreign surfaces such as microorganisms and tissue damaged by physical or chemical injury. C5a binds to at least two seven-transmembrane domain receptors, C5aR (C5R1, CD88) and C5L2 (gpr77).Expression of C5L2 (C5a Anaphylatoxin Receptor 2) can be found in various tissues and cells such as astrocytes, neutrophils/macrophages, mast cells, immature dendritic cells, as well as in the brain, lung, heart, kidney, liver, ovary or testis.The C5L2 receptor is a G-protein coupled receptor clustered with other chemoattractant receptors, such as type-II angiotensin-II receptor, bradykinin receptors, the formyl peptide receptor family, ChemR23 and several orphan GPCRs. A small number of single-nucleotide polymorphisms (SNP) have been found in the receptor gene. C5L2 has two synonymous SNPs at 614G/A and 860C, however there are no known associations between these SNPs and human disease.Although C5L2 has the conventional structure of a GPCR, studies have shown that C5L2 does not couple to G proteins owing to the lack of a highly conserved Asp-Arg-Tyr motif, found in the third TM domain1.

Applications may also work in :

IFC LCI WB

Supplier :

Alomone Labs

Target :

C5a anaphylatoxin chemotactic receptor 2, C5a anaphylatoxin receptor 2, Complement component 5a receptor 2, G-protein coupled receptor 77, C5L2, GPR77

Short Description :

A Rabbit Polyclonal Antibody to C5a Anaphylatoxin Receptor 2

Long Description :

Anti-Human C5aR2 (extracellular) Antibody (#AAR-033) is a highly specific antibody directed against an epitope of human C5a anaphylatoxin receptor 2 (C5aR2, C5L2). The antibody can be used in western blot analysis. It recognizes an extracellular epitope and is likely to detect the receptor in living cells. It has been designed to recognize C5L2 from human samples only.

Negative Control :

BLP-AR033

Positive Control :

Synonyms :

C5a anaphylatoxin chemotactic receptor 2, C5a anaphylatoxin receptor 2, Complement component 5a receptor 2, G-protein coupled receptor 77, C5L2, GPR77

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

C5AR2

AB Specifictiy :

Will not recognize C5a Anaphylatoxin Chemotactic Receptor 2 from rat or mouse samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage