Anti-Human C5aR1 (extracellular) Antibody | Alomone Labs AAR-032 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Human C5aR1 (extracellular) Antibody

catalog :

AAR-032

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of Human K562 myelogenous leukemia (lanes 1 and 4), MEG-01 chronic myelogenous leukemia cell (lanes 2 and 5), and THP-1 acute monocytic leukemia (lanes 3 and 6) cell lysates: - 1-3. Anti-Human C5aR1 (extracellular) Antibody (#AAR-032), (1:200).4-6. Anti-Human C5aR1 (extracellular) Antibody, preincubated with Human C5aR1 (extracellular) Blocking Peptide (#BLP-AR032).

image 2 :

Peptide (C)DKDTLDLNTPVDK corresponding to amino acid residues 16-28 of human C5aanaphylatoxin receptor (AccessionP21730). Extracellular N-terminus.

image 3 :

Cell surface detection of C5aR1 in HL-60 intact living cells: - ___Unstained cells.___Cells + Anti-Human C5aR1 (extracellular) Antibody(#AAR-032) (10 g/1x106cells).

product information

CAT :

AAR-032

SKU :

AAR-032-CF_0.2 ml

Product Name :

Anti-Human C5aR1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P21730

Applications :

IFC LCI WB

Reactivity :

Human

Host :

Rabbit

Blocking Peptide :

BLP-AR032

Homology :

Human only Not recommended for use with rat and mouse samples

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)DKDTLDLNTPVDK, corresponding to amino acid residues 16-28 of human C5a anaphylatoxin receptor

Product Page - Scientific background :

The innate immune system identifies pathogens by recognizing known elements. This response ultimately leads to the production of proinflammatory substances and the activation of phagocytic neutrophils and macrophages1. Activation of the complement system is an important initial even in the response against pathogens. This system includes some thirty different proteins that act together to form a complex1.C5a is one of the most potent inflammatory peptides generated by complement activation. This peptide has many functions such as acting as a chemoattractant for neutrophils2,3. C5a acts as a vasodilator3,4 and activates the coagulation pathway3,5 just to name a few.C5a exerts its numerous actions by binding to C5aR and C5L2. C5aR belongs to the superfamily of G-protein coupled receptors (GPCRs). Like all members, C5aR has seven transmembrane domains, an extracellular N-terminus and an intracellular C-terminal tail. C5aR can form homodimers6-8 or heteromer with CCR5, chemokine receptor, for example8,9. Activation of C5aR by C5a binding causes Ca2+ mobilization10.C5aR expression is detected in neutrophils, eisinophils, basophils and monocytes3,11,12. The receptor is also expressed in lung and liver13,14.Blocking C5aR by peptides or organic small molecules is in the pipeline for treating chronic inflammatory disorders/diseases like arthritis, inflammatory bowel disease and lung/liver injuries8.

Applications may also work in :

IFC LCI WB

Supplier :

Alomone Labs

Target :

C5a1 receptor, C5a anaphylatoxin chemotactic receptor 1, C5a anaphylatoxin receptor, C5R1, Complement component 5a receptor 1, CD88

Short Description :

A Rabbit Polyclonal Antibody to C5a Anaphylatoxin Receptor

Long Description :

Anti-Human C5aR1 (extracellular) Antibody (#AAR-032) is a highly specific antibody directed against an epitope of human C5a anaphylatoxin receptor. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize C5aR from human samples only.

Negative Control :

BLP-AR032

Positive Control :

Synonyms :

C5a1 receptor, C5a anaphylatoxin chemotactic receptor 1, C5a anaphylatoxin receptor, C5R1, Complement component 5a receptor 1, CD88

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

C5AR1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage