Anti-Human C3aR (extracellular) Antibody | Alomone Labs AAR-031 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Human C3aR (extracellular) Antibody

catalog :

AAR-031

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of differentiated HL-60 cell lysate: - 1. Anti-Human C3aR (extracellular) Antibody (#AAR-031), (1:200).2. Anti-Human C3aR (extracellular) Antibody, preincubated with Human C3aR (extracellular) Blocking Peptide (#BLP-AR031).

image 2 :

Peptide (C)EDHETSPLDNSD corresponding to amino acid residues 276-287 of human C3a anaphylatoxin receptor (AccessionQ16581). 2ndextracellular loop.

image 3 :

Cell surface detection of C3aR in differentiated HL-60 living cells: - ___Unstained cells.___Cells + Anti-Human C3aR(extracellular) Antibody (#AAR-031) (5-10 g/1x106cells).

product information

CAT :

AAR-031

SKU :

AAR-031-CF_0.2 ml

Product Name :

Anti-Human C3aR (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q16581

Applications :

IFC LCI WB

Reactivity :

Human

Host :

Rabbit

Blocking Peptide :

BLP-AR031

Homology :

Recommended to use with human samples only

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)EDHETSPLDNSD, corresponding to amino acid residues 276-287 of human C3a anaphylatoxin receptor

Product Page - Scientific background :

The innate immune system identifies pathogens by recognizing known elements. This response ultimately leads to the production of proinflammatory substances and the activation of phagocytic neutrophils and macrophages1. Activation of the complement system is an important initial even in the response against pathogens. This system includes some thirty different proteins that act together to form a complex1.Three different pathways are known to activate the complement. The C3 complement plays a key role in all three pathways leading to the activation of the complement. Produced in the liver, C3 is the most abundant complement protein in the serum. It is composed of two polypeptides, an α and β chain. Cleavage of C3 leads to the production of C3b and C3a1.C3a binds to C3a receptor, a G-protein coupled receptor which couples to Gi upon activation. Like all other members of the superfamily, C3a receptor has seven transmembrane domains with an extracellular N-terminus and intracellular C-terminal tail1. The receptor has two glycosylation sites, an unusually large extracellular loop with a sulfated tyrosine residue important for ligand recognition and binding2,3.C3a receptor is expressed in cells of myeloid origin like neutrophils, macrophages and dendritic cells to name a few2. It is also detected in astrocytes from inflamed brain4, endothelial cells5 and activated human T cells6. mRNA of C3a receptor is detected in lung, liver, kidney, brain heart, muscle and testis2,7.

Applications may also work in :

IFC LCI WB

Supplier :

Alomone Labs

Target :

C3a anaphylatoxin chemotactic receptor, C3a anaphylatoxin receptor, Complement component 3a receptor 1, C3a receptor, C3AR1, C3R1, AZ3B, HNFAG09

Short Description :

A Rabbit Polyclonal Antibody to C3a Anaphylatoxin Receptor

Long Description :

Anti-Human C3aR (extracellular) Antibody (#AAR-031) is a highly specific antibody directed against an epitope of human C3a anaphylatoxin receptor. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize C3a receptor from human samples only.

Negative Control :

BLP-AR031

Positive Control :

Synonyms :

C3a anaphylatoxin chemotactic receptor, C3a anaphylatoxin receptor, Complement component 3a receptor 1, C3a receptor, C3AR1, C3R1, AZ3B, HNFAG09

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

C3AR1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage