Anti-Apelin/APJ Receptor (extracellular) Antibody | Alomone Labs AAR-030 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Apelin/APJ Receptor (extracellular) Antibody

catalog :

AAR-030

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain membrane (lanes 1 and 4), rat pituitary cell line (GH3) lysate (lanes 2 and 5) and rat heart membrane (lanes 3 and 6): - 1-3. Anti-Apelin/APJ Receptor (extracellular) Antibody (#AAR-030), (1:200).4-6. Anti-Apelin/APJ Receptor (extracellular) Antibody, preincubated with Apelin/APJ Receptor (extracellular) Blocking Peptide (#BLP-AR030).

image 2 :

Expression of Apelin receptor in mouse brain - Immunohistochemical staining of mouse cerebellum free floating frozen sections using Anti-Apelin/APJ Receptor (extracellular) Antibody (#AAR-030), (1:600). A. Apelin receptor staining (green) appears in neurons of the Purkinje cell (PC) (horizontal arrows in C) layer and in the dendritic tree (vertical arrow in C) in the molecular layer (MOL). B. Nuclei staining using DAPI as the counterstain (blue). C. Merged image of panels A and B.

product information

CAT :

AAR-030

SKU :

AAR-030-CF_0.2 ml

Product Name :

Anti-Apelin/APJ Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9JHG3

Applications :

IF IFC IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-AR030

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

DGYNYYGADNQSEC, corresponding to amino acid residues 4-17 of rat apelin receptor

Product Page - Scientific background :

The apelin receptor (APJ) is a member of the G-protein coupled receptor gene family, encoded by the APLNR gene. The apelin receptor and its endogenous ligand, apelin, have been suggested to act as key mediators of physiological responses to multiple homeostatic actions, including cardiovascular control, water balance, hypothalamic-pituitary-adrenal (HPA) axis regulation and metabolic homeostasis.Apelin receptor structure contains seven hydrophobic transmembrane domains, with several sites for phosphorylation by protein kinase A, and sites for palmitoylation and glycosylation. The N-terminal glycosylation is implicated in the receptor's expression, stability, correct folding of the protein and ligand binding. The C-terminal palmitoylation has been reported to play a role in membrane association. These modifications can influence the internalization, dimerization and ligand binding of the receptor. Human apelin receptor shares 92% homology with mouse receptor and 90% homology with rat receptor.Apelin expression can be detected in several brain regions including the human caudate nucleus, corpus callosum, hippocampus, substantia nigra, subthalamic nucleus, medulla and spinal cord. Apelin receptor is also expressed in the spleen, placenta and cardiovascular tissues and small amounts in the small intestine, lung, stomach, colonic mucosa and ovary.

Applications may also work in :

IF IFC IHC WB

Supplier :

Alomone Labs

Target :

APLNR, Angiotensin receptor-like 1, AGTRL1, G-protein coupled receptor APJ, GPCR34

Short Description :

A Rabbit Polyclonal Antibody to Apelin Receptor

Long Description :

Anti-Apelin/APJ Receptor (extracellular) Antibody (#AAR-030) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, live cell flow cytometry, and immunohistochemistry applications. It has been designed to recognize Apelin receptor from mouse, rat, and human samples.

Negative Control :

BLP-AR030

Positive Control :

Synonyms :

APLNR, Angiotensin receptor-like 1, AGTRL1, G-protein coupled receptor APJ, GPCR34

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

APLNR

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:600

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage