Anti-β1-Adrenergic Receptor (extracellular) Antibody | Alomone Labs AAR-023 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-β1-Adrenergic Receptor (extracellular) Antibody

catalog :

AAR-023

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Saunders S, Hutchinson D, Britton F, Liu L, Markus I, Sandow S, et al. Effect of β1 /β2 -adrenoceptor blockade on β3 -adrenoceptor activity in the rat cremaster muscle artery. Br J Pharmacol. 2021;178:1789-1804 pubmed publisher
Evans A, Ardestani P, Yi B, Park H, Lam R, Shamloo M. Beta-adrenergic receptor antagonism is proinflammatory and exacerbates neuroinflammation in a mouse model of Alzheimer's Disease. Neurobiol Dis. 2020;146:105089 pubmed publisher

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat lung (lanes 3 and 6) membranes: - 1-3. Anti-β1-Adrenergic Receptor (extracellular) Antibody (#AAR-023), (1:200).4-6. Anti-β1-Adrenergic Receptor (extracellular) Antibody, preincubated with β1-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR023).

image 2 :

Expression of β1-Adrenoceptor in rat and mouse brain - Immunohistochemical staining of rat dorsal raphe nucleus and mouse cerebellum using Anti-β1-Adrenergic Receptor (extracellular) Antibody (#AAR-023), (1:400). A. β1-Adrenoceptor staining (red) in rat dorsal raphe nucleus appears in neurons (horizontal arrows). C. β1-Adrenoceptor in mouse cerebellum staining (red) is detected in granule cells (horizontal arrows) and in the Purkinje cell layer (vertical arrow). B, D. Nuclear staining using DAPI as the counterstain (blue).

image 3 :

Expression of ?1-Adrenoceptor in rat and mouse brain - Immunohistochemical staining of rat dorsal raphe nucleus and mouse cerebellum usingAnti-?1-Adrenergic Receptor (extracellular)Antibody (#AAR-023) (1:400).A.?1-Adrenoceptor staining (red)in rat dorsal raphe nucleus appears in neurons (horizontal arrows).C.?1-Adrenoceptorin mouse cerebellum staining (red) is detected in granule cells (horizontal arrows) and in the Purkinje cell layer (vertical arrow).B D. Nuclear staining using DAPI as the counterstain (blue).

product information

CAT :

AAR-023

SKU :

AAR-023-CF_0.2 ml

Product Name :

Anti-β1-Adrenergic Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P18090

Applications :

IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-AR023

Homology :

Rat, Mouse, human - 14/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RAESDEARR(S)YNDPK, corresponding to amino acid residues 200-214 of rat ADRB1

Product Page - Scientific background :

β-Adrenergic receptors (βAR) belong to the superfamily of G-protein coupled receptors (GPCRs). βAR stimulation by the sympathetic nervous system or circulating catecholamines is involved in peripheral blood circulation, metabolic regulation, muscle contraction, and central neural activities. In the heart, acute βAR stimulation serves as the most powerful means to regulate cardiac output in response to a fight-or-flight situation.There are three βAR subtypes, β1AR, β2AR and β3AR, in cardiac myocytes1. The receptors consist of seven membrane-spanning domains, three intra- and three extracellular loops, an extracellular N-terminal domain, and an intracellular C-terminal tail2.β1AR is the predominant receptor subtype expressed in human cardiomyocytes. β1AR is also expressed in the kidney3. It has been implicated in adipogenesis and memory formation4.β1AR has been widely connected with heart disease. Both the downregulation and overstimulation of β1AR have been connected to myocyte apoptosis. In addition, chronic heart failure is characterized by abnormalities in β1AR signal transduction. It was demonstrated that heart failure is also inducible in animal models by overexpression of components of the β1AR signal transduction cascade. Transgenic mice overexpressing β1AR develop, with aging, heart failure similar to that observed in dilated cardiomyopathy in humans5.

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Beta-1 adrenoceptor, Beta-1 adrenoreceptor, ADRB1, ADRB1R, B1AR

Short Description :

A Rabbit Polyclonal Antibody to β1-Adrenergic Receptor

Long Description :

Anti-β1-Adrenergic Receptor (extracellular) Antibody (#AAR-023) is a highly specific antibody directed against an extracellular epitope the rat β1-adrenoceptor. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize β1AR from mouse, rat and human samples.

Negative Control :

BLP-AR023

Positive Control :

Synonyms :

Beta-1 adrenoceptor, Beta-1 adrenoreceptor, ADRB1, ADRB1R, B1AR

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ADRB1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage