antibody

Anti-β1-Adrenergic Receptor (extracellular)- mFluor™ Violet 450 Antibody

AAR-023-V

polyclonal

domestic rabbit

nonconjugated

human, mouse, rat

AAR-023-V_15 mcl

Anti-β1-Adrenergic Receptor (extracellular)- mFluor™ Violet 450 Antibody

Antibodies

Antibodies

Polyclonal

P18090

FC LCI

Human Rat Mouse

Rabbit

BLP-AR023

Rat, Mouse, human - 14/15 amino acid residues identical.

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

15 µl or 50 µl double distilled water (DDW), depending on the sample size.

1 mg/ml

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

1% BSA, 0.05% NaN3

NA

mFluor™ Violet 450. Maximum absorption 405 nm and maximum emission 445 nm. These spectral characteristics make it an excellent replacement for Pacific Blue™ or Brilliant Violet™ 421 dyes. For flow cytometry applications, the labeled antibody can be detected using the 405 nm laser lane and a filter set of 450/50.

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Rat

(C)RAESDEARR(S)YNDP, corresponding to amino acid residues 200-214 of rat ADRB1

β-Adrenergic receptors (βAR) belong to the superfamily of G-protein coupled receptors (GPCRs). βAR stimulation by the sympathetic nervous system or circulating catecholamines is involved in peripheral blood circulation, metabolic regulation, muscle contraction, and central neural activities. In the heart, acute βAR stimulation serves as the most powerful means to regulate cardiac output in response to a fight-or-flight situation. There are three βAR subtypes, β1AR, β2AR and β3AR, in cardiac myocytes1. The receptors consist of seven membrane-spanning domains, three intra- and three extracellular loops, an extracellular N-terminal domain, and an intracellular C-terminal tail2. β1AR is the predominant receptor subtype expressed in human cardiomyocytes. β1AR is also expressed in the kidney3. It has been implicated in adipogenesis and memory formation4. β1AR has been widely connected with heart disease. Both the downregulation and overstimulation of β1AR have been connected to myocyte apoptosis. In addition, chronic heart failure is characterized by abnormalities in β1AR signal transduction. It was demonstrated that heart failure is also inducible in animal models by overexpression of components of the β1AR signal transduction cascade. Transgenic mice overexpressing β1AR develop, with aging, heart failure similar to that observed in dilated cardiomyopathy in humans5.

FC LCI

Alomone Labs

Beta-1 adrenoceptor, Beta-1 adrenoreceptor, ADRB1, ADRB1R, B1AR

A Rabbit Polyclonal Antibody to β1-Adrenergic Receptor conjugated to mFluor™ Violet 450 fluorophore.

Anti-β1-Adrenergic Receptor (extracellular) Antibody (#AAR-023) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and indirect live cell flow cytometry applications. It has been designed to recognize β1-Adrenergic Receptor from rat, mouse, and human samples. Anti-β1-Adrenergic Receptor (extracellular)- mFluor™ Violet 450 Antibody (#AAR-023-V) is directly conjugated to mFluor™ Violet 450 fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. mFluor™ Violet 450 dye has a maximum absorption of 405 nm and maximum emission of 445 nm. These spectral characteristics make it an excellent replacement for Pacific Blue™ or Brilliant Violet™ 421 dyes. For flow cytometry applications, the labeled antibody can be detected using the 405 nm laser lane and a filter set of 450/50.

RIC-001-APC

Beta-1 adrenoceptor, Beta-1 adrenoreceptor, ADRB1, ADRB1R, B1AR

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

Affinity purified on immobilized antigen.

Rabbit IgG Isotype Control-mFluor™ Violet 450

41116161

Western blot analysis (unlabeled antibody, #AAR-023), and direct flow cytometry (labeled antibody).