antibody

Anti-β1-Adrenergic Receptor (extracellular)-ATTO Fluor-488 Antibody

AAR-023-AG

polyclonal

domestic rabbit

ATTO 488

NA

mouse, rat

immunohistochemistry, immunocytochemistry

AAR-023-AG_50 mcl

Anti-β1-Adrenergic Receptor (extracellular)-ATTO Fluor-488 Antibody

Antibodies

Antibodies

Polyclonal

P18090

IF IHC

Rat Mouse

Rabbit

BLP-AR023

Mouse, human - 14/15 amino acid residues identical

PBS pH7.4, 1% BSA with 0.05% sodium azide

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

50 µl double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1% BSA, 0.05% NaN3

2nd extracellular loop

ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Rat

(C)RAESDEARR(S)YNDP, corresponding to amino acid residues 200-214 of rat ADRB1

β-Adrenergic receptors control key physiological functions through signals encoded by catecholamine hormones and neurotransmitters to activate intracellular signaling pathways. These receptors belong to the amine receptor cluster of rhodopsin-like family of G-protein coupled receptors (GPCRs).The β1 adrenergic receptor (β1AR) is comprised of an extracellular N-terminus, seven membrane spanning loops (TM1-7) and an intracellular C-terminus1.β1AR couples to the stimulatory G protein Gs and thereby activates adenylyl cyclase. β1AR also couples to the inhibitory G protein Gi and can induce G protein independent signaling. Differential activation of these pathways can be modulated by different ligands2.β1AR is widely expressed in cardiac myocytes and is regulated by the microRNA (miRNA) let-7e. Following acute myocardial infraction (AMI) in rats, expression of β1AR is significantly up-regulated while the expression of miRNA let-7a (as well as miRNAs c, d, e and i) is notably down-regulated 6 and 24 hours post myocardial infarction. Blocking the up-regulation of β1AR can serve as a novel therapeutic approach for the treatment of AMI-induced arrhythmia3.β1AR is also implicated in the pathophysiology of Alzheimer's disease (AD). Administration of a partial β1AR agonist, behavioral deficits in a mouse model of AD are restored and amyloid beta and tau levels decrease as measured by regional immunohistochemistry4.

IF IHC

Alomone Labs

Beta-1 adrenoceptor, Beta-1 adrenoreceptor, ADRB1, ADRB1R, B1AR

A Rabbit Polyclonal Antibody to β1-Adrenergic Receptor Conjugated to the Fluorescent Dye ATTO-488

Anti-β1-Adrenergic Receptor (extracellular) Antibody (#AAR-023) is a highly specific antibody directed against an extracellular epitope the rat β1-adrenoceptor. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize β1AR from mouse, rat and human samples. Anti-β1-Adrenergic Receptor (extracellular)-ATTO Fluor-488 Antibody (#AAR-023-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-β1-Adrenergic Receptor (extracellular)-ATTO Fluor-488 Antibody has been tested in immunohistochemistry applications and is especially suited for experiments requiring simultaneous labeling of different markers.

RIC-001-AG

NA

Beta-1 adrenoceptor, Beta-1 adrenoreceptor, ADRB1, ADRB1R, B1AR

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

ADRB1

Lyophilized powder

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis (unlabeled antibody, #AAR-023), and immunohistochemistry (labeled antibody).

1 µg peptide per 1 µg antibody

1:60

Contact Alomone for information